Method of screening drug

ABSTRACT

This invention provides a method of screening for pharmaceutical agents that regulate at least one of metabolism or growth of, or infection by microorganisms, which comprises selecting ligands of an Lrp-like protein based on 3D structural information of the protein.

TECHNICAL FIELD

The present invention relates to a method of screening forpharmaceutical agents that regulate at least one of metabolism or growthof, or infection by microorganisms.

BACKGROUND ART

Analyses of genomic DNA sequences of microorganisms have revealed that agroup of proteins that are very similar to each other are commonly foundin many microorganisms. Among this group of proteins, function of E.coli Lrp (leucine responsive regulatory protein) has been elucidated tothe greatest extent. Another E. coli protein, AsnC, is classified intothe same group. The amino acid sequences of the two proteins aresimilar, and their functions too are similar. Thus, AsnC is regarded asan Lrp-like protein. Hereinafter, these homologous proteins arecollectively referred to as the “group of Lrp proteins” or “Lrp-likeproteins.”

In response to extracellular nutritional conditions indicated by theextracellular concentration of leucine, E. coli Lrp regulates itsmetabolism. This mechanism is referred to as the “feast/famine”regulation. If extracellular nutritional conditions improve, E. colishifts from the “famine” state to the “feast” state. Specifically, E.coli suspends movement to other sites, actively absorbs nutrientsthrough the cell membrane, terminates autotrophic metabolic pathways foramino acid synthesis and the like, and initiates cell division andgrowth. Finally, its infectivity too is changed. Experimentally, severaldozen metabolism-associated operons have been identified as beingregulated by Lrp, the number of such genes may well reach 10% of thewhole genes coded in the E. coli genome. Similarly, the AsnC protein, anE. coli Lrp-like protein, regulates amino acid synthesis and DNAreplication in response to the concentration of asparagine.

In order to achieve such regulation, Lrp-like proteins recognizepromoter DNA sites positioned upstream of the genes regulated, therebyactivating or inactivating transcription of these genes. Associationwith or dissociation from promoter DNA sites by Lrp-like proteins isregulated, in many cases, by extracellular nutritional conditions. Typesof small molecules that mediate extracellular nutritional conditions(hereafter referred to as “ligands”) will introduce structural changesinto Lrp-like proteins, thereby affecting their ability to associatewith promoter DNA sites.

However, 3D (i.e., three dimensional) structural information of Lrp-likeproteins adequate for elucidating the aforementioned mechanisms, or thatadequate for modeling or screening of novel ligands was not elucidated.In general, X-ray diffraction analysis, NMR spectroscopy, electronmicroscopy, and the like are available for studying the 3D structures ofproteins. However, the sizes of the target molecules are restricted inNMR spectroscopy and the accuracy of electron microscopy isinsufficient. Thus, X-ray diffraction analysis is the method mostsuitable for analyzing the 3D structures of assemblies of Lrp-likeproteins. Crystallization of proteins, that is required in X-raydiffraction analysis, is generally difficult.

Accordingly, crystallization of E. coli Lrp has been attempted in Europeand the United States, but there has been no report of success. AnLrp-like protein derived from another type of microorganism has beencrystallized in Europe, and the 3D combination of secondary structuralelements has been reported for the protein. However, the resolution isas low as approximately 3 Å, and the R factor is higher than 30%. Nodetailed experimental information is available for the geometry of aminoacid side chains, which is important for analyzing interaction withpotential ligands.

DISCLOSURE OF THE INVENTION

The aim of the present invention is by analyzing the 3D structure of anLrp-like protein using the 3D coordinates of atoms in side chainsthereof, to identify part of the 3D structure that can be targeted uponmodeling or screening ligands. Furthermore, the present inventionprovides a method of screening for pharmaceutical agents that canregulate one of metabolism or growth of, or infection by microorganismsbased on the aforementioned 3D structural information.

By intensive studies, the present inventors have succeeded incrystallizing one of the fourteen Lrp-like proteins present inPyrococcus sp. OT3 (alias: Pyrococcus horikoshii or Pyrococcus shinkaii,Japan Collection of Microorganisms; registration number: JCM 9974), andthe 3D structure thereof has been determined at a high resolution usingX-ray diffraction analysis. Based on this 3D structural information, thepresent inventors have established a method of screening forpharmaceutical agents that will function as antibiotics by targetingtranscriptional regulation by Lrp-like proteins of the genes that areinvolved in metabolism or growth of, or infection by the microorganisms.

More specifically, the present invention relates to the following (1) to(12).

(1) A method of screening for pharmaceutical agents that regulate atleast one of metabolism or growth of, or infection by microorganisms,which comprises selecting ligands of an Lrp-like protein based on the 3Dstructural information of the protein.

(2) The method according to (1) above, wherein the 3D structuralinformation is information concerning gaps or pockets generated byassembly of Lrp-like protein monomers.

(3) The method according to (1) or (2) above, wherein the 3D structuralinformation is information obtained from 3D atomic coordinatesdetermined by X-ray diffraction analysis.

(4) The method according to (3) above, which comprises the followingsteps:

-   -   1) determination of the 3D atomic coordinates of the Lrp-like        protein by applying X-ray diffraction analysis to the protein        crystallized;    -   2) processing of the 3D atomic coordinates using a program        designed for analyzing protein 3D structures, thereby obtaining        graphical and/or numerical information concerning the 3D        structure of the Lrp-like protein;    -   3) deduction of the 3D structural information on gaps or pockets        generated by assembling of monomers of the Lrp-like protein, by        using the graphical and/or numerical information; and    -   4) identification of ligands which will interact with the        Lrp-like protein and thus will be usable as pharmaceutical        agents, by using the 3D structural information.

(5) The method according to any one of (1) to (4) above, wherein thepharmaceutical agents that regulate at least one of metabolism or growthof, or infection by microorganisms, are antimicrobial agents.

(6) The method according to any one of (1) to (5) above, wherein theLrp-like protein is derived from a microorganism belonging to the genusPyrococcus.

(7) The method according to (6) above, wherein the microorganism isPyrococcus sp. OT3 (JCM 9974).

(8) The method according to (7) above, wherein the 3D atomic coordinatescomprise the data shown in Table 1.

(9) A recording medium in which the following a) and/or b) are recorded:

-   -   a) 3D atomic coordinates of an Lrp-like protein; and    -   b) graphical and/or numerical information concerning the 3D        structure of an Lrp-like protein obtained by analyzing the 3D        atomic coordinates using a program designed for analyzing        protein 3D structures.

(10) The recording medium according to (9) above, wherein the Lrp-likeprotein is derived from a microorganism belonging to the genusPyrococcus.

(11) The recording medium according to (10) above, wherein themicroorganism is Pyrococcus sp. OT3 (JCM 9974).

(12) The recording medium according to (11) above, wherein the 3D atomiccoordinates comprise the data shown in Table 1.

The present invention relates to a method of screening forpharmaceutical agents that regulate at least one of metabolism or growthof, or infection by microorganisms, wherein a ligand interacting with anLrp-like protein is selectively identified using 3D structuralinformation concerning the aforementioned protein.

Hereafter, the present invention is described in detail.

1. An Lrp-Like Protein

In the present invention, the term “Lrp-like protein” refers to theleucine responsive regulatory protein (Lrp) of E. coli or a homologuethereof derived from any microorganism. Lrp is an E. coli protein, whichregulates metabolism of this organism in response to the extracellularconcentration of leucine. The term “Lrp homologues” refers to a group ofproteins whose amino acid sequences and functions (i.e., response toextracellular nutritions and regulation of metabolisms) are similar tothose of E. coli Lrp. Examples of Lrp homologues include E. coli AsnCprotein, LrpA of the genus Pyrococcus, and Sa-lrp of the genusSulfolobus.

Lrp-like proteins function by binding to promoter DNA sites positionedupstream of the genes regulated by Lrp-like proteins, thereby activatingor inactivating transcription of these genes. It is known thatassociation with or dissociation from promoter DNA sites by Lrp-likeproteins is regulated by types of small molecules that reflect theextracellular nutritional condition. These small molecules causestructural changes of the Lrp-like proteins, thereby affecting theirabilities to associate with the promoter DNAs. In this specification,“small molecules that bind to Lrp-like proteins and cause structuralchanges of the proteins” are referred to as “ligands”.

Application of the present invention is not restricted to any particularLrp-like protein, but this invention can be applied to any organismwhich has an Lrp-like protein. Such microorganisms include, E. coli,Bacillus subtilis, Thermoplasma, and Sulfolobus. Microorganisms in thegenus Pyrococcus, particularly Pyrococcus sp. OT3 (JCM 9974), can betargeted.

2. Three Dimensional Structural Information

In the present invention, the term “3D structural information of anLrp-like protein” refers to information concerning the 3D structure ofan Lrp-like protein in a crystal. This 3D structural informationincludes information concerning both the monomeric form of the Lrp-likeprotein and its assembly form. The information also includes informationconcerning the Lrp-like protein both in a form associating with a ligandand the form dissociating.

In the present invention, information concerning gaps or pockets createdupon assembling of monomers of an Lrp-like protein is particularlyuseful. The term “gap” refers to an empty space created between monomersof an Lrp-like protein, between atoms or between amino acid residues.The term “pocket” refers to another empty space created around thecenter of the assembly of an Lrp-like protein. These spaces areimportant for interaction with “ligands”, and thus are used forscreening of pharmaceutical agents.

The 3D structural information can be obtained using, for example, X-raydiffraction analysis, NMR spectroscopy, or electron microscopy. Sincethe size of molecules that can be analyzed by NMR spectroscopy islimited, and since the resolution obtainable by electron microscopy islimited, use of X-ray diffraction analysis is appropriate. In X-raydiffraction analysis, phase of diffraction spots can be determined, ifnot only the original crystals of protein but also their isomorphousderivatives with heavy-atoms are made. For obtaining such derivatives,the original crystals are soaked into at least two solutions eachcontaining a heavy atom (for example, platinum or gold). Diffractionfrom the original and derivatives are analyzed as a set.

Using the obtained diffraction, an electron density map is computed,using commercially available programs such as programs in the CCP4package, and the 3D atomic coordinates can be determined, so that theybest fits the electron density map. An example of such “3D atomiccoordinates” is described using the Protein Data Bank (PDB) format inTable 1. The PDB format is an international standard for describing the3D coordinates of atoms in proteins.

Subsequently, the 3D atomic coordinates can be processed using a programdesigned for analyzing protein 3D structures, thereby obtaininggraphical information and/or numerical information concerning the 3Dstructure of an Lrp-like protein. Examples of such a “program designedfor analyzing protein 3D structures” include CCP4, O, X-PLOR, MolScript,Insight II, and Grasp Programs. The term “graphical information” refersto any information represented as images, e.g., in the form of ribbondiagrams (e.g., FIGS. 3 and 4) or in the form of wire models (e.g., FIG.2). Similarly, “numerical information” refers to any informationexpressed by combining numbers, e.g., values such as those defining thediameters of crystals, the widths or depths of pockets or gaps.

3. Screening for Ligands

The atomic coordinates and the graphical and/or numerical informationconcerning the 3D structure of the Lrp-like protein will determine alarge continuous empty space expanding from a pocket to gaps, therebydefining the coordinates of hydrophobic or hydrophilic chemical groupsfacing the empty space. Thus, they can be useful for screening forligands that will act on the Lrp-like protein.

As is described in the “Examples”, association with a natural ligand canintroduce 3D structural changes to an Lrp-like protein, affectingassociation with or dissociation from a promoter DNA site by theLrp-like protein. Accordingly, such a ligand can be used as apharmaceutical agent that artificially regulates metabolism or growthof, or infection by the microorganism. Preferably, the pharmaceuticalagent will terminate metabolism or growth of, or infection by themicroorganism, thereby preventing pathogenic function of themicroorganism.

No Lrp-like protein has been found in an eukaryote, including of humans.Thus, it is likely that a ligand interacting with an Lrp-like proteinwill not cause any serious side effect to humans. If such a ligandfunction as an antibiotic, its target protein and mechanism of actionwill become different from those of known antibiotics. This mightprevent infection by microorganisms which are resistant to knownantibiotics; such infection is becoming more and more serious.

4. A Recording Medium

The present invention also provides a medium in which the 3D structuralinformation of the Lrp-like protein is recorded. Specifically, thismedium records the following a) and/or b) thereon:

-   -   a) the 3D atomic coordinates of the Lrp-like protein; and    -   b) graphical and/or numerical information concerning the 3D        structure of the Lrp-like protein obtained by analyzing the 3D        atomic coordinates using a program designed for analyzing        protein 3D structures.

In this specification, the 3D structural information of a particularLrp-like protein derived from Pyrococcus sp. OT3 is shown as apreferable example of such data recorded on a medium. The term “medium”indicates any one of the media that can record the aforementionedinformation, such as a CD-ROM, an optical disk, an optical file, a MO, aminidisk, a flexible disk, and a computer hard disk.

The aforementioned medium provides information necessary for screeningfor ligands interacting with Lrp-like proteins, and thus can be used asa tool for screening for pharmaceutical agents that regulate at leastone of metabolism or growth of, or infection by microorganisms.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows crystals of the Lrp-like protein.

FIG. 2 shows part of the electron density map calculated for theLrp-like protein (i.e., the 2 Fo-Fc map; counter level: 1.0) and thefinal model of the protein fitted into the map.

FIG. 3 shows two views of a ribbon diagram showing the 3D structure ofan octameric assembly of the Lrp-like protein, determined by X-raycrystallography (see FIG. 2).

FIG. 4 shows two views of another ribbon diagram showing the 3Dstructure of a dimer of the Lrp-like protein in the crystal.

FIG. 5 shows profiles of gel filtration of the Lrp-like protein:black-purified by a mild method (1), red-purified by a rigorouscondition (2): see Examples for understanding these purificationmethods.

FIG. 6 indicates the electron densities (red) identified ascorresponding to two molecules of the natural ligand most likely, thathad been absorbed into octamers of the Lrp-like protein: subfigure b isa side view, while the top views shown in (a), and subfigure c is anenlargement of (b).

FIG. 7 shows crystals of the Lrp-like protein before or immediatelyafter (upper), or several hours after (lower) soaked into solutioncontaining 10 mM Leu (a) or 10 mM Asp (b). FIG. 8 shows profiles of gelfiltration of the Lrp-like protein in in the presence of various aminoacids (a and b) or some other compounds (c), i.e., malic acid (red),2-oxoglutaric acid (green), and oxaloacetic acid (blue), or in theirabsence (black).

This description includes the contents disclosed in the description ofJapanese Patent Application No. 2001-384683, which is a prioritydocument of the present application.

BEST MODE FOR CARRYING OUT THE INVENTION

The present invention is hereafter described in more detail, referringto particular examples, although the present invention is not limited tothis particular form.

EXAMPLE 1 Crystallization of the Lrp-Like Protein

(1) Construction of a vector for expressing the Lrp-like protein

The following primers were synthesized in order to amplify a DNAfragment coding the Lrp-like protein (llp) gene by PCR using the genomicDNA of Pyrococcus sp. OT3 (JCM 9974) as the template. PCR was carriedout using the reaction solution, 20 μl, LA Tag (Takara Shuzo Co., Ltd.)and GeneAmp PCR system 9600 (Perkin Elmer): each cycle consisting ofdenaturation at 94° C., annealing at 55° C., and elongation at 72° C.,and repeating this cycle 30 times.

The amplified DNA fragment was cleaved using restriction enzymes NdeI(Takara Shuzo Co., Ltd.) and BamHI (Takara Shuzo Co., Ltd.), and thefragment coding the gene was separated from the rest by electrophoresisusing an agarose gel. The DNA fragment coding the llp gene was ligatedwith an expression vector, pET3 (Novagen), cleaved using restrictionenzymes, NdeI and BamHI, thereby constructing a expression vector(pET3-LRPS01): 10 μl of the reaction solution, 660 mM Tris-HCl buffer(pH 7.6) containing 66 mM MgCl₂, 100 mM DTT, and 1 mM ATP, was kept at16° C. overnight, in the presence of T4 DNA ligase (Takara Shuzo Co.,Ltd.). Primers: Forward Strand: (SEQ ID NO: 1)5′-TGGTGATGACATATGGTGACGGCCTTTATCCTG-3′ Reverse Strand: (SEQ ID NO: 2)5!-GAACGGATCCATCAAATTGCTATCATAGTCGAGGTC-3′(2) Expression of the Lrp-Like Protein Using E. Coli Cells

The vector designed for expressing the llp gene, pET3-LRPS01, wasintroduced into E. coli cells strain BL21 (DE3) (Novagen). The E. colicells were cultured in 8 ml of the 2×YT medium containing 50 mg/mlampicillin at 37° C. overnight, inoculated into 8 l of the same medium,and allowed to grow until the absorbance of the medium reachedapproximately 0.75 at 600 nm. Subsequently, 2 mMisopropylthiogalactoside (IPTG) was added to induce expression of theLrp-like protein. After additional culture for 4 hours, cells werecollected by centrifugation at 500 rpm for 5 minutes using a centrifuge(Beckman Avanti J-25, 7,).

(3) Purification of the Lrp-Like Protein

The cells expressing the Lrp-like protein were suspended into 250 ml of100 mM Tris-HCl buffer (pH 7.0) containing 1 mM EDTA, and treated usinga French press (SLM). After centrifugation for 30 minutes at 4° C. at25,000 rpm, using a centrifuge (Beckman Avanti J-25) and a rotor(JA-25.5), the supernatant was subjected to a thermal treatment at 75°C. for 1 hour. After another centrifugation at 4° C. at 25,000 rpm for30 minutes, the supernatant was dialyzed against 30 mM Tris-HCl buffer(pH 7.0), and subjected to further purification: by twice repeating thefollowing process, i.e., anion exchange column chromatography followedby gel filtration.

The supernatant was applied to an anion exchanger (1.6×18 cm, ResourceQ, Pharmacia), in a column, equilibrated with 30 mM Tris-HCl buffer (pH7.0), and the column was washed with the same buffer. Subsequently, theprotein was eluted with an linear gradient, 0-2 M of NaCl in 30 mMTris-HCl buffer (pH 7.0), with a flow rate of 2 ml/min, using FPLCcolumn (Pharmacia). Each solution, The solution eluted from the columnwas fractionated into fractions of 4 ml, was collected, each, and thoseidentified as containing the Lrp-like protein were identified using SDSelectrophoresis and were stored.

The stored protein once stored was dialyzed against 100 mM Tris-HCl (pH7.0) and subject to gel filtration. The protein solution was applied toa gel column (2.6×60 cm, Superdex 75, Pharmacia) in a column (2.6×60cm), equilibrated with 100 mM Tris-HCl buffer (pH 7.0), and eluted withthe same buffer with the flow rate 1 ml/min using the (FPLC system,(Pharmacia). The fractions, obtained (each of, 2 ml each,) weresubjected to SDS electrophoresis, and those containing the Lrp-likeprotein were identified and stored. The above processes of anionexchange chromatography and gel filtration were alternately carried outtwo times alternatively, and the protein was purified to a quality withwhich so that a single band was observed when subjected to SDSelectrophoresis.

(4) Crystallization of the Lrp-Like Protein

The purified Lrp-like protein, 20 mg/ml in 10 mM Tris-HCl buffer (pH7.0), was used for crystallization. Crystallization was carried outusing 24-well crystallization plates (Hampton Research) by avapor-diffusion method, using a sitting-drop technique. Specifically, 4(1 of the protein solution was mixed with 4 (1 of the same solution asplaced in the reservoir (100 mM citrate buffer (pH 4.0) containing 10%by weight of PEG 6000 and 1.0 M lithium chloride). This crystallizationsolution was allowed to equilibrated with 0.8 ml of the 100 mM citratebuffer (pH 4.0) containing 10% by weight of PEG 6000 and 1.0 M lithiumchloride placed in the reservoir solution, by vapor diffusion at aconstant temperature of 5° C. In approximately a week, crystals inpyramid-like hexagonal forms (of the sizes of approximately 0.2 mm) wereobtained (FIG. 1).

EXAMPLE 2 Analysis of the 3D Structure of the Lrp-Like Protein

(1) X-Ray Diffraction Analysis

In order to determine the 3D structure of the Lrp-like protein by aheavy atom isomorphous replacement method using heavy atom derivatives,the original crystals obtained (see Example 1) were soaked intosolutions containing 10 mM K₂[Pt(CN)₆] or 0.1 mM K[AuCl₄] at 5° C. forapproximately 2 days.

Four sets of diffraction were measured from a total of four sets, i.e.,two sets of the original crystals and one set of each two sets, eachfrom of the two heavy atom derivatives (platinum and or gold), weremeasured by using 1) an ordinary (i.e., conventional a rotating anodetype) X-ray source and 2) two sets from the original crystals using theordinary X-ray source or a synchlotron radiation X-ray source.

1) Analysis Using an Ordinary (i.e., a Conventional Rotating Anode Type)X-Ray Source

Diffraction from a set of the original crystals, and the two sets ofheavy atom derivatives (platinum and gold), were measured, using anordinary (i.e., a conventional rotating anode type) X-ray source at roomtemperature. Each crystal was placed inside a quartz capillary (1.5 mmin the diameter, TOHO), and an excess solvent was removed. After sealingthe both ends of the capillary with wax, the capillary was placed insidean X-ray diffractometer (R-AXIS IV, Rigaku Denki). An X-ray source wasproduced by rotating thean anode at 50 kV and 100 mA (UltraX 18, RigakuDenki), and the diffraction was recorded using a diffractometer.

2) Analysis Using a Synchlotron Radiation X-Ray Source

In order to obtain more diffraction containing information at higherresolutions, the original crystals were frozen and kept in this statewhile measuring their diffraction using a synchrotron radiation X-raysource, i.e., the Hyogo beamline BL24XU at using a synchrotron radiationX-ray source, i.e., the Hyogo beamline BL24XU at SPring8 (Hyogo, Japan).The crystals were soaked into a solution containing a cryo-protectant,100 mM citrate buffer (pH 4.0) containing 20%, by weight, of glycerol,14%, by weight, of PEG 6000, and 1.0 M lithium chloride for severalminutes, placed inside a 0.2 mm-mount loop (Cryoloop, Hampton Research),and frozen using liquid nitrogen at −196° C. During the measurements,the crystals were kept at another temperature of −173° C. using a device(Rigaku Denki) designed to spray cold air to crystals. Diffraction wasrecorded by a diffractometer (R-AXIS IV, Rigaku Denki) using synchrotronX-ray of a wave length of 0.834 Å.

(2) Data Analysis

The recorded diffraction was processed using a program designed for thediffractometer and the programs in the CCP4 package (CollaborativeComputational Project, Number 4, Acta Crystallographica D50, 760-763,1994). Consequently, the space group of the crystals was identified toP3₂21 or P3₁21, and the unit cell lengths were identified: a=b=96.3 Å,and c=97.1 Å. Based on these lengths, it was concluded that eachasymmetric unit of these crystals contained three to six molecules ofthe Lrp-like protein.

(3) Building an Initial Model

The diffraction from the original and the two heavy atom derivatives(platinum and gold) were integrated using programs in the CCP4 package,and used for the subsequent computational analysis. Positions of theheavy atoms in the derivatives were determined using differencePatterson maps. The gold and platinum derivatives, respectively, had twoand three heavy metal atoms per asymmetric units. Numbers, positions,and occupancies of the heavy atoms were refined using the MLPHAREprogram in the CCP4 package. The overall figure of merit at this stagewas calculated as 0.43 at 3.0 Å.

By using the phase obtained as above, and by using the isomorphousreplacement method, an electron density map was calculated. By examiningthe map, the phase was improved by the methods of solvent flattening andhistogram matching, using the DM program in the CCP4 package. In therevised electron density map, calculated using the improved phase,electron densities corresponding to right-handed α-helices wereidentified appropriately only when four molecules of the protein wereassumed in each asymmetric unit and when these units were related by thespace group P3₂21. A model was made by assembling four identicalmonomers (each consisting of 72 alanine residues), so that the modelwould best fit to the electron density map using program 0 (Jones T. A.,Zou J. Y., Cowan S. W., and Kjeldgaard M., Improved methods for bindingprotein models in electron density maps and the location of errors inthese models, Acta Crystallographica A47, 110-119, 1991).

(4) Refinement of the Model

The initial model was refined using the X-PLORE program (Brünger, A.X-PLORE v3.1 Manual (Yale University, New Haven, 1992)), so that themodel would best satisfy both the diffraction data experimentallyobtained and the standard geometric parameters (Engh and Huber) thatwere expected for protein 3D structures in general. The model wasfurther refined using program 0 to correct the part of the model wherelarge deviations from the electron density map were observed. Until thisstage, the four monomers in the asymmetric unit were kept identical toeach other. The R factor indicating the difference between theexperimental data and the model was 39.1% at this stage.

In order to further refine the model, the high-resolution data obtainedat SPring8 BL24XU were used. The aforementioned alanine model wasrefined using the X-PLORE program using data up to 3.0 Å, and other dataup to 2.0 Å were incorporated while improving the phase using the wARPprogram (Perrakis, A., Sixma, T. K., Wilson, K. S., and Lamzin, V. S.,wARP: Improvement and extension of crystallographic phases by weightedaveraging multiple refined dummy atomic models, Acta CrystallographicaD53, 448-455, 1997). Using the improved phase, a new electron densitymap was calculated, where electron densities of most of the atoms in theprotein, including the atoms of side chains of the amino acid residues,were clearly identified. Based on this electron density map, another 3Dstructural model was made by including the amino acid side chains.

Further refinements were carried out at a resolution of 1.8 Å, bymodeling solvent molecules so that there would match with electrondensities that were clearly identified but not interpretable as anyatoms in the protein, which will be discussed further in the followingparagraph. At the final stage of refinement, the four monomers in theasymmetric unit were allowed to adopt non-identical 3D structures.

(5) Determination of the Final Model

The final model (FIGS. 2 and 3) was assembled by four independentmonomers of the Lrp-like protein. Each monomer comprises all theresidues except for the first amino acid residue, i.e., methionine, atthe N-terminal end. The R factor, evaluating the consistency between theexperimental data (20-1.8 Å) and the final model, was calculated as21.2%, indicating a high accuracy of the 3D model. The PROCHECK programin the CCP4 package was used to calculate a Ramachandran plot. In theplot, 98.5% of all the residues were found in energetically-most stableregions. The average temperature factor (B-factor) was as small as 17.3,indicating that all the atoms were determined unambiguously, except forsome side chains, which were positioned on the surface, and thusexpected as possessing real flexibilities for movements.

EXAMPLE 3 Analysis of the Assembling of the Lrp-Like Protein by GelFiltration

It has been reported that many Lrp-like proteins derived frommicroorganisms, including E. coli form dimers or tetramers in solution.The Lrp-like protein expressed in E. coli was analyzed by gel filtrationin order to estimate sizes of its assemblies in solution.

Specifically, the Lrp-like protein purified for the crystallization wasdiluted by 50 mM Tris-HCl buffer (pH 7.0) containing 300 mM sodiumchloride, yielding a concentration of approximately 100 μM. Gelfiltration was carried out at a flow rate 1 ml/min (LC Module I plusSystem, Waters) using a matrix (Protein Pak 125, 7.8×300 mm, Waters) andthe same buffer, i.e., 50 mM Tris-HCl buffer (pH 7.0) containing 300 mMsodium chloride. Retention of the protein was recorded by measuring theUV absorption at 220 nm.

A multiple number of peaks were observed (FIG. 5, purification 1). Ingeneral upon gel filtration, assemblies of larger sizes will be retainedfor a shorter time. Accordingly, the first peak (peak 1 in FIG. 5) mightcorrespond to an octamer, and other peaks retained longer shouldcorrespond to smaller assemblies. This protein forms a variety ofassemblies up to an octamer depending on the condition. The function ofthe octamer may be different from those of smaller assemblies.

EXAMPLE 4 Analysis of the 3D Coordinates of Atoms in the Lrp-LikeProtein

(1) The 3D Coordinates of Atoms in the Lrp-Like Protein

The atomic coordinates of the 3D structure of the Lrp-like proteindetermined are shown using the protein data bank (PDB) format (Table 1),describing the four independent monomers of the protein (A to D) in theasymmetric unit and 196 water molecules. Lines 1 to 7 of Table 1 specifythe crystal symmetry to repeat the asymmetric unit, as is generally thecase of any crystals. From line 8, eleven parameters (i)-(xi) describedfrom left to right are: (i) serial numbers of the atoms (1 to 2,556);(ii) types of atoms, e.g., C the carbon, further differentiated as, forexample, CA, CB, indicating the positions inside the amino acidresidues, also including information concerning chemical bondings; (iii)assignments of the atoms to 20 types of amino acid residues, using the 3letter code, e.g., VAL for valine) or, (HOH) for the atoms belonging notto amino acid residues but to water molecules, and, in addition, theirassignments to the four monomers (A to D) or to water molecule (W); (iv)amino acid residue numbers counted from the N terminus in each monomer,or numbers 1 to 196 for the water molecules; (v) the X coordinates inangstrom; (vi) the Y coordinates in angstrom; (vii) the Z coordinates inangstrom; (viii) occupancies (i.e., probabilities of the atoms occupyingthe given coordinates); (ix) isotropic temperature factors (i.e.,measures of the flexibilities of atoms for thermal movements); (x) theatomic numbers defined by the periodic table (e.g., 6 for C, 7 for N,and 8 for 0); and (xi) types of the atoms, the same as in (ii) (e.g., C,O, and N).

The atom types (e.g., C, O) described above are in general thosedescribed by a nomenclature of the IUPAC-IUB Nomenclature, but in (ii),A, B, C, D, E, Z, and H were used (e.g., CA or CB) instead of α, β, γ,δ, ε, ζ, and η. The additional oxygen atoms present at the C terminiwere labeled as OXT (OT).

(2) Analysis of the 3D Coordinates

1) Assembling by the Lrp-Like Protein

By analyzing the data shown in Table 1, it was found that each monomerof the Lrp-like protein is composed of a four-stranded β sheet and twoα-helices (FIG. 4). Each pair of monomers assemble into a dimer, byforming a single β-sheet composed of eight strands, i.e., four strandsfrom each monomer (FIG. 4). In the crystal, each set of 4 dimers furtherassemble, thereby forming a disk-like octamer (FIG. 3). The diameter ofthe octamer disk is approximately 60 Å and the thickness thereof isapproximately 40 Å.

2) Characteristics of the 3D Structure

At the center of the disk-like octamer of the Lrp-like protein, thereexists a pocket, the overall shape resembling a Japanese 50-yen coinhaving a hole in the center. An empty space extends from this centralpocket to the four gaps, each formed between a pair of dimers, as awhole, forming a cross-like shape (see, FIG. 3 upper). The boundary ofthis empty space, the types of amino acid side chains surrounding theempty space, and the coordinates of these side chains conform the 3Dstructural information critical for screening for ligands interactingwith the Lrp-like protein.

The boundary of the central pocket resembles a cylinder of the diameterapproximately 12 Å and the height approximately 30 Å, which is largeenough for containing two amino acid molecules. Eight sets of amino acidside chains, each from each of the eight monomers assembling, valine 9,glutamic acid 37, tyrosine 38, methionine 68, and serine 70, are facingthis pocket: (here numbers, such as 9, 37, or 38, are those of aminoacid residues, counted from the N terminus in each monomer (see Table1). Also, residues 37, 38, and 68 are positioned at the borderconnecting the pocket to the gaps.

Each of the four gaps formed between the pairs of the dimers is largeenough to accommodate two amino acids, or able to do so by beingexpanded by the two amino acids. Each gap was surrounded by two sets ofside chains of the two dimers, glutamic acid 15, valine 33, tyrosine 35,aspartic acid 39, leucine 49, aspartic acid 53, isoleucine 56, threonine57, arginine 61, threonines 69 and 71, and isoleucines 73 and 75.

3) Application of the 3D Information to Screening for Ligands

If a ligand binds to the empty space extending from the pocket to thegaps, and if the ligand is completely complementary to part of the spaceand thus able to fit into, the ligand will stabilize the octamer therebyactivating the functions of Lrp-like protein as an octamer. However, ifthe ligand is larger than the space and binds there with a highconstant, it will enlarge the empty space, thereby dissociating theoctamer terminating the functions of the Lrp-like protein as an octamer,and activating functions as smaller assemblies. Most likely, he emptyspace will be the site for interacting with natural ligands.

As has been described, graphical and/or numerical information of theLrp-like protein obtained from the 3D coordinates shown in Table 1determine the boundary of the empty space extending from the pocket tothe gaps and specify the positions of atomic groups, hydrophobic orhydrophilic, facing the space. Thus, this information provides a usefulplatform for screening for ligands interacting with the Lrp-likeprotein.

EXAMPLE 5 The Position of a Natural Ligand Present in the Crystal ofLrp-Like Protein

(1) The Presence of a Natural Ligand in the Crystal of the Lrp-LikeProtein

The presence of a natural ligand in the crystal of the Lrp-like protein(Example 1) was found and confirmed as described below.

1) Purification of the Lrp-Like Protein Using a Method Different fromWhat was Described in Example 1

The protein was purified by a more rigorous method with additional usesof ammonium sulfate fractionation and hydrophobic column chromatography.Ammonium sulfate fractionation was carried out after the heat treatmentand centrifugation. Namely, ammonium sulfate was added to thesupernatant, yielding 40% saturation. The solution was centrifuged at 4°C. at 18,000 rpm for 15 minutes. Ammonium sulfate was further added tothe supernatant, yielding 80% saturation. After the centrifugation at 4°C. at 18,000 rpm for 15 minutes, the sediment was dissolved in 30 ml of30 mM Tris-HCl buffer (pH 7.0) and dialyzed against 30 mM Tris-HClbuffer (pH 7.0). Hydrophobic column chromatography was carried out afterthe anion exchange column chromatography and the gel filtrationdescribed in Example 1. Namely, after the gel filtration, ammoniumsulfate was added to the protein solution, yielding a concentration of1.6 M, and applied to a column (1.6×18 cm), containing Butyl-Toyopearl650 M (Tosoh) pre-equilibrated with 50 mM Tris-HCl buffer (pH 7.0)containing 1.5 M ammonium sulfate. The column was washed with the samebuffer. Subsequently, the protein was eluted using a line, 1.5-0 M,gradient of ammonium sulfate in 50 mM Tris-HCl buffer (pH 7.0) with theflow rate 2 ml/min: the FPLC column (Pharmacia) was used. Fractions, 4ml each, were subjected to SDS electrophoresis and those identified ascontaining the Lrp-like protein were stored.

2) Changes Between Assembly Forms

By the rigours purification the apparent molecular weight in solution ofthe Lrp-like protein was changed, suggesting changes in the assemblyform, using gel filtration (FIG. 5, purification 2). Compared with theoriginal method employed in Example 1, in the modified method theprotein is exposed to the environments accerelating, in general,denaturation of proteins. Thus, the change in the assembly form can beexplained, if a ligand present in E. coli cells was binding to theLrp-like protein, thereby stabilizing its assembling, after thepurification by the original method, while, if the ligand was removedfrom the protein by the modified method. If the above idea that anatural ligand which can accelerate assembling of the Lrp-like proteinis present in E. coli cells and that this ligand was incorporated intothe assembly of the Lrp-like protein upon expression of this protein inE. coli cells, it is expected that the crystals obtained using theLrp-like protein purified by the original method should have been thoseof the complex of the Lrp-like protein and the E. coli ligand ratherthan those of the Lrp-like protein alone.

(2) The Position of the Natural Ligand Present in the Crystal of theLrp-Like Protein

The electron density map was re-analyzed, seeking for a density whichmight correspond to the E. coli ligand. Other than the densities clearlycorresponding to the protein, there existed extra densities, which thenwere interpreted as those of the 196 water molecules. By re-examiningthese extra densities, electron densities which likely corresponded totwo molecules of the ligand were identified, inside the gaps formedbetween the dimers (FIG. 6).

As has been described, what can be determined experimentally by X-raydiffraction analysis is an electron density map, and the 3D coordinatesobtained best fitting the map are still a model, (FIG. 2). A number ofwater molecules are contained in crystals, in general. It is extremelydifficult to identify molecules as small as water in an electron densitymap. Of the atoms constituting a water molecule, the two hydrogensdiffract X-rays very poorly, and thus, in short, they are invisible byX-ray analysis. Consequently, in order to identity a water molecule, apoint-like density of the oxygen atom isolated from any other densityneeds to be identified in the electron density map. The extra densitiespresent in the electron density map of the Lrp-like protein was firstinterpreted as 196 water molecules, or more precisely 196 oxygen atoms.If a natural ligand originated in E. coli. cells are present in thecrystal it should be larger than a single water molecule, correspondingto one or more of such electron densities.

In order to identify the electron density of the ligand, water moleculesmodeled to relatively low electron densities were removed. Of these 66were found inside the pocket at the center of the protein octamer. Thiswas necessary to remove distortion of the map caused by possiblemisidentification of these atoms. Starting with this model, the processof 3D structure refinement was repeated several times, and thedifference between the model and the electron density map (hereafterreferred to as the difference Fourier map) was computed, expecting thata density should emerge, corresponding to the ligand. At each cycle,high densities newly found in the difference Fourier map (at counterlevels of three or higher) were examined thoroughly. To these densities,which were isolated or which were close enough to hydrophilic groups inthe protein so that ionic/hydrogen bonding was possible, new watermolecules were modeled. Finally, an electron density larger than thatexpected for a water molecule, was identified in each asymmetric unit.Since an octamer is composed of two such asymmetric units two suchdensities were found in the octamer (FIG. 6). These two densities wereinterpreted as those of two molecules of the ligand, each positionedinside the gap formed between a pair of dimers. Each electron densityhas a size equivalent with four or more water molecules, or equivalentwith a single molecule of a medium sized amino acid such as valine orisoleucine.

EXAMPLE 6 Interaction Between the Lrp-Like Protein and Natural Ligandsin Solution

It has been confirmed that various amino acids and, in addition, someother biomolecules (e.g., metabolic intermediates) can act as naturalligands on assemblies of the Lrp-like protein.

(1) Depolarization of the Crystals by Amino Acids

It has been known that the regularity inside a crystal might beaffected, if the structure of the protein changes upon interaction witha ligand which is incorporated from outside the crystal, therebydepolarizing the crystal. Thus, crystals of the Lrp-like protein weresoaked into solutions, each containing one of twenty amino acids at thesame concentration 10 mM. With isoleucine, valine, methionine, andleucine, in several hours the crystals depolarized (FIG. 7). With theother amino acids, no change was observed. It is important to note thatthe changes caused by the four amino acids to the 3D structure of theprotein are not necessarily the same, and that the other amino acids,although they showed no effect, might not have diffused into thecrystal, and thus these 1 might function as ligands in solution.

(2) Analysis Using Gel Filtration

Possible changes in assemblies of the Lrp-like protein induced byinteraction with amino acids and metabolic intermediates were analyzedin solution using gel filtration.

1) Experimental Procedure

Gel filtration was carried out with the flow rate 1 m/min, using amatrix (Protein Pak 125, 7.8×300 mm, Waters) and 50 mM Tris-HCl buffer(pH 7.0) containing 300 mM sodium chloride. Flow of the proteins wasmonitored by measuring the UV absorbance at 220 nm (LC Module I plussystem, Waters). The Lrp-like protein purified by method 2, i.e., therigorous method, were dissolved in 50 mM Tris-HCl buffer (pH 7.0)containing 200 mM sodium chloride, and kept at −80° C. until it wasdiluted by 10-fold with 50 mM Tris-HCl buffer (pH 7.0) containing 300 mMsodium chloride, and mixed with each potential ligand for at least 30minutes at room temperature (about 25° C.). In the mixture, the monomerconcentration of the Lrp-like protein was approximately 100 μM. While,the concentration of each-amino acid was 1 mM and that of 5 metabolicintermediates, i.e., malic acid, 2-oxoglutaric acid, oxaloacetic acid,pyruvic acid, and 3-phosphoglyceric acid, was 10 mM.

2) Results

When isoleucine was mixed with the protein, the profile of gelfiltration changed, increasing the second peak. While, hen valine wasmixed, the third peak increased (FIG. 8 a). Six other amino acids, i.e.,methionine, arginine, leucine, phenylalanine, alanine, and threonine,showed effects of increasing the fourth peak (FIG. 8 b). None of theother twelve amino acids showed any significant effect. When one of thethree metabolic intermediates, each containing four or more carbon atoms(i.e., malic acid, 2-oxoglutaric acid, and oxaloacetic acid) was mixed,the third peak increased (FIG. 8 c). In contrast, no significant effectwas observed with two other metabolic intermediates, each containingthree carbon atoms (i.e., pyruvic acid and 3-phosphoglyceric acid).

Peaks associated with smaller retention time should correspond toassemblies of larger molecular weights. Thus it is concluded thatisoleucine accelerates assembling of the protein, six other amino acids,i.e., methionine, arginine, leucine, phenylalanine, alanine, andthreonine, accelerate disassembling of the protein, and valine and threemetabolic intermediates (malic acid, 2-oxoglutaric acid, and oxaloaceticacid) have intermediate effects. TABLE 1 The atomic coordinates of the3D structure of the Lrp-like protein ORIGX1  1.000000  0.000000 0.000000   0.00000 ORIGX2  0.000000  1.000000  0.000000   0.00000ORIGX3  0.000000  0.000000  1.000000   0.00000 CRYST1 96.345  96.345 97.056  90.00  90.00 120.00 SCALE1  0.010379  0.005993  0.000000  0.00000 SCALE2  0.000000  0.011985  0.000000   0.00000 SCALE3 0.000000  0.000000  0.010303   0.00000 ATOM 1 CB VAL A 2 −2.073 48.3597.218 1.00 7.25 6 C ATOM 2 CG1 VAL A 2 −0.814 47.539 6.836 1.00 8.49 6 CATOM 3 CG2 VAL A 2 −3.080 48.341 6.088 1.00 7.16 6 C ATOM 4 C VAL A 2−1.718 47.700 9.595 1.00 7.20 6 C ATOM 5 O VAL A 2 −1.191 48.725 10.0101.00 8.49 8 O ATOM 6 N VAL A 2 −4.005 48.669 8.832 1.00 7.12 7 N ATOM 7CA VAL A 2 −2.752 47.840 8.490 1.00 7.39 6 C ATOM 8 N THR A 3 −1.37646.456 9.992 1.00 7.51 7 N ATOM 9 CA THR A 3 −0.236 46.219 10.863 1.008.84 6 C ATOM 10 CB THR A 3 −0.541 45.367 12.111 1.00 9.16 6 C ATOM 11OG1 THR A 3 −1.620 46.010 12.820 1.00 9.25 8 O ATOM 12 CG2 THR A 3 0.61645.208 13.110 1.00 8.76 6 C ATOM 13 C THR A 3 0.836 45.449 10.073 1.006.50 6 C ATOM 14 O THR A 3 0.454 44.528 9.380 1.00 4.22 8 O ATOM 15 NALA A 4 2.098 45.907 10.132 1.00 6.94 7 N ATOM 16 CA ALA A 4 3.10145.068 9.420 1.00 6.58 6 C ATOM 17 CB ALA A 4 3.286 45.669 8.021 1.006.97 6 C ATOM 18 C ALA A 4 4.402 45.022 10.211 1.00 7.80 6 C ATOM 19 OALA A 4 4.635 45.828 11.124 1.00 7.68 8 O ATOM 20 N PHE A 5 5.272 44.0589.877 1.00 5.61 7 N ATOM 21 CA PHE A 5 6.561 44.001 10.554 1.00 7.24 6 CATOM 22 CB PHE A 5 6.806 42.707 11.355 1.00 6.59 6 C ATOM 23 CG PHE A 55.969 42.618 12.605 1.00 7.48 6 C ATOM 24 CD1 PHE A 5 4.628 42.27112.533 1.00 7.25 6 C ATOM 25 CD2 PHE A 5 6.511 42.995 13.830 1.00 8.05 6C ATOM 26 CE1 PHE A 5 3.833 42.218 13.670 1.00 8.84 6 C ATOM 27 CE2 PHEA 5 5.734 42.907 14.983 1.00 6.40 6 C ATOM 28 CZ PHE A 5 4.430 42.49314.877 1.00 7.39 6 C ATOM 29 C PHE A 5 7.648 44.035 9.432 1.00 7.62 6 CATOM 30 O PHE A 5 7.556 43.301 8.456 1.00 4.61 8 O ATOM 31 N ILE A 68.535 45.005 9.654 1.00 7.29 7 N ATOM 32 CA ILE A 6 9.625 45.211 8.7291.00 7.19 6 C ATOM 33 CB ILE A 6 9.692 46.692 8.314 1.00 5.96 6 C ATOM34 CG2 ILE A 6 10.728 46.809 7.191 1.00 5.34 6 C ATOM 35 CG1 ILE A 68.337 47.188 7.774 1.00 7.13 6 C ATOM 36 CD1 ILE A 6 8.404 48.686 7.4321.00 9.39 6 C ATOM 37 C ILE A 6 10.957 44.758 9.348 1.00 6.37 6 C ATOM38 O ILE A 6 11.373 45.243 10.412 1.00 6.22 8 O ATOM 39 N LEU A 7 11.56843.783 8.725 1.00 7.34 7 N ATOM 40 CA LEU A 7 12.832 43.204 9.137 1.009.69 6 C ATOM 41 CB LEU A 7 12.897 41.701 8.927 1.00 12.76 6 C ATOM 42CG LEU A 7 11.781 40.816 9.451 1.00 17.25 6 C ATOM 43 CD1 LEU A 7 12.03839.325 9.258 1.00 18.40 6 C ATOM 44 CD2 LEU A 7 11.550 41.078 10.9221.00 17.00 6 C ATOM 45 C LEU A 7 13.973 43.797 8.292 1.00 9.33 6 C ATOM46 O LEU A 7 13.711 44.012 7.118 1.00 7.50 8 O ATOM 47 N MET A 8 15.01244.351 8.930 1.00 9.25 7 N ATOM 48 CA MET A 8 16.013 45.098 8.227 1.0011.96 6 C ATOM 49 CB MET A 8 15.954 46.584 8.663 1.00 13.86 6 C ATOM 50CG MET A 8 14.610 47.251 8.492 1.00 18.88 6 C ATOM 51 SD MET A 8 14.49048.890 9.189 1.00 16.93 16 S ATOM 52 CE MET A 8 13.643 48.403 10.6981.00 23.90 6 C ATOM 53 C MET A 8 17.458 44.653 8.580 1.00 14.57 6 C ATOM54 O MET A 8 17.730 44.249 9.707 1.00 12.69 8 O ATOM 55 N VAL A 9 18.35744.743 7.615 1.00 13.97 7 N ATOM 56 CA VAL A 9 19.787 44.503 7.800 1.0014.26 6 C ATOM 57 CB VAL A 9 20.417 43.674 6.668 1.00 16.26 6 C ATOM 58CG1 VAL A 9 21.972 43.738 6.697 1.00 16.52 6 C ATOM 59 CG2 VAL A 919.994 42.242 6.760 1.00 14.67 6 C ATOM 60 C VAL A 9 20.346 45.902 7.8071.00 15.95 6 C ATOM 61 O VAL A 9 19.933 46.661 6.900 1.00 20.59 8 O ATOM62 N THR A 10 21.152 46.424 8.712 1.00 15.01 7 N ATOM 63 CA THR A 1021.660 47.783 8.609 1.00 13.75 6 C ATOM 64 CB THR A 10 21.540 48.6759.859 1.00 13.36 6 C ATOM 65 OG1 THR A 10 22.194 48.066 11.001 1.0011.93 8 O ATOM 66 CG2 THR A 10 20.070 48.913 10.248 1.00 13.19 6 C ATOM67 C THR A 10 23.190 47.710 8.376 1.00 15.09 6 C ATOM 68 O THR A 1023.728 46.650 8.648 1.00 15.54 8 O ATOM 69 N ALA A 11 23.809 48.8278.039 1.00 16.40 7 N ATOM 70 CA ALA A 11 25.306 48.728 7.997 1.00 17.236 C ATOM 71 CB ALA A 11 25.835 50.065 7.564 1.00 16.26 6 C ATOM 72 C ALAA 11 25.722 48.352 9.413 1.00 17.14 6 C ATOM 73 O ALA A 11 25.090 48.78210.388 1.00 16.25 8 O ATOM 74 N ALA A 12 26.758 47.550 9.599 1.00 17.627 N ATOM 75 CA ALA A 12 27.170 47.140 10.952 1.00 17.18 6 C ATOM 76 CBALA A 12 28.389 46.228 10.790 1.00 18.07 6 C ATOM 77 C ALA A 12 27.43148.312 11.870 1.00 16.00 6 C ATOM 78 O ALA A 12 28.132 49.260 11.5281.00 14.87 8 O ATOM 79 N GLY A 13 26.855 48.330 13.093 1.00 14.71 7 NATOM 80 CA GLY A 13 27.171 49.433 14.022 1.00 14.32 6 C ATOM 81 C GLY A13 26.236 50.639 13.929 1.00 15.21 6 C ATOM 82 O GLY A 13 26.360 51.57514.690 1.00 15.60 8 O ATOM 83 N LYS A 14 25.288 50.635 13.004 1.00 15.717 N ATOM 84 CA LYS A 14 24.320 5.724 12.873 1.00 16.25 6 C ATOM 85 CBLYS A 14 24.207 52.109 11.375 1.00 17.80 6 C ATOM 86 CG LYS A 14 25.56652.622 10.845 1.00 21.86 6 C ATOM 87 CD LYS A 14 25.885 53.964 11.4841.00 21.55 6 C ATOM 88 CE LYS A 14 27.300 54.385 11.090 1.00 24.44 6 CATOM 89 NZ LYS A 14 27.432 55.819 11.485 1.00 25.93 7 N ATOM 90 C LYS A14 22.920 51.288 13.302 1.00 14.39 6 C ATOM 91 O LYS A 14 22.013 52.11113.269 1.00 15.68 8 O ATOM 92 N GLU A 15 22.738 50.034 13.653 1.00 13.017 N ATOM 93 CA GLU A 15 21.393 49.571 14.001 1.00 15.99 6 C ATOM 94 CBGLU A 15 21.452 48.127 14.521 1.00 17.36 6 C ATOM 95 CG GLU A 15 22.24848.147 15.825 1.00 25.39 6 C ATOM 96 CD GLU A 15 22.807 46.808 16.2461.00 31.23 6 C ATOM 97 OE1 GLU A 15 22.135 45.923 15.626 1.00 35.29 8 OATOM 98 OE2 GLU A 15 23.723 46.692 17.119 1.00 30.70 8 O ATOM 99 C GLU A15 20.705 50.444 15.061 1.00 15.48 6 C ATOM 100 O GLU A 15 19.530 50.76114.944 1.00 12.54 8 O ATOM 101 N ARG A 16 21.415 50.780 16.143 1.0016.35 7 N ATOM 102 CA ARG A 16 20.787 51.555 17.210 1.00 19.56 6 C ATOM103 CB ARG A 16 21.667 51.542 18.462 1.00 23.74 6 C ATOM 104 CG ARG A 1621.326 52.436 19.616 1.00 29.54 6 C ATOM 105 CD ARG A 16 21.727 53.91019.477 1.00 35.88 6 C ATOM 106 NE ARG A 16 21.060 54.702 20.540 1.0041.43 7 N ATOM 107 CZ ARG A 16 21.427 54.741 21.819 1.00 43.05 6 C ATOM108 NH1 ARG A 16 22.488 54.052 22.253 1.00 45.29 7 N ATOM 109 NH2 ARG A16 20.720 55.492 22.656 1.00 44.30 7 N ATOM 110 C ARG A 16 20.424 52.94716.752 1.00 18.28 6 C ATOM 111 O ARG A 16 19.346 53.412 17.143 1.0016.68 8 O ATOM 112 N GLU A 17 21.313 53.637 16.018 1.00 16.79 7 N ATOM113 CA GLU A 17 20.956 54.980 15.563 1.00 15.83 6 C ATOM 114 CB GLU A 1722.123 55.767 15.012 1.00 18.26 6 C ATOM 115 CG GLU A 17 22.870 55.07913.891 1.00 21.97 6 C ATOM 116 CD GLU A 17 22.345 55.501 12.538 1.0025.19 6 C ATOM 117 OE1 GLU A 17 21.453 56.393 12.521 1.00 26.12 8 O ATOM118 OE2 GLU A 17 22.822 54.970 11.512 1.00 27.57 8 O ATOM 119 C GLU A 1719.819 54.887 14.538 1.00 14.37 6 C ATOM 120 O GLU A 17 18.952 55.77314.540 1.00 12.63 8 O ATOM 121 N VAL A 18 19.744 53.871 13.711 1.0011.94 7 N ATOM 122 CA VAL A 18 18.618 53.738 12.799 1.00 12.82 6 C ATOM123 CB VAL A 18 18.806 52.622 11.773 1.00 13.64 6 C ATOM 124 CG1 VAL A18 17.503 52.290 11.014 1.00 10.60 6 C ATOM 125 CG2 VAL A 18 19.88253.077 10.757 1.00 13.42 6 C ATOM 126 C VAL A 18 17.359 53.493 13.6311.00 14.02 6 C ATOM 127 O VAL A 18 16.319 54.112 13.357 1.00 14.65 8 OATOM 128 N MET A 19 17.442 52.601 14.594 1.00 13.17 7 N ATOM 129 CA META 19 16.271 52.267 15.425 1.00 13.50 6 C ATOM 130 CB MET A 19 16.68151.279 16.512 1.00 14.15 6 C ATOM 131 CG MET A 19 15.459 50.893 17.3281.00 15.47 6 C ATOM 132 SD MET A 19 15.745 49.773 18.610 1.00 16.28 16 SATOM 133 CE MET A 19 16.553 50.600 19.921 1.00 18.98 6 C ATOM 134 C META 19 15.711 53.484 16.132 1.00 14.53 6 C ATOM 135 O MET A 19 14.48653.703 16.175 1.00 13.43 8 O ATOM 136 N GLU A 20 16.611 54.320 16.6491.00 14.59 7 N ATOM 137 CA GLU A 20 16.163 55.531 17.322 1.00 18.00 6 CATOM 138 CB GLU A 20 17.306 56.289 18.025 1.00 20.38 6 C ATOM 139 CG GLUA 20 17.844 55.506 19.220 1.00 23.46 6 C ATOM 140 CD GLU A 20 16.82155.001 20.202 1.00 27.70 6 C ATOM 141 OE1 GLU A 20 15.771 55.673 20.4051.00 31.74 8 O ATOM 142 OE2 GLU A 20 17.017 53.914 20.797 1.00 29.38 8 OATOM 143 C GLU A 20 15.420 56.452 16.373 1.00 17.62 6 C ATOM 144 O GLU A20 14.387 56.983 16.753 1.00 17.09 8 O ATOM 145 N LYS A 21 15.914 56.70815.172 1.00 16.59 7 N ATOM 146 CA LYS A 21 15.260 57.495 14.164 1.0015.48 6 C ATOM 147 CB LYS A 21 16.106 57.424 12.866 1.00 15.60 6 C ATOM148 CG LYS A 21 17.132 58.542 12.743 1.00 19.35 6 C ATOM 149 CD LYS A 2118.166 58.319 11.668 1.00 20.39 6 C ATOM 150 CE LYS A 21 19.434 59.16211.914 1.00 23.18 6 C ATOM 151 NZ LYS A 21 20.429 58.883 10.813 1.0021.66 7 N ATOM 152 C LYS A 21 13.879 56.889 13.850 1.00 14.96 6 C ATOM153 O LYS A 21 12.905 57.633 13.658 1.00 15.15 8 O ATOM 154 N LEU A 2213.759 55.566 13.682 1.00 10.45 7 N ATOM 155 CA LEU A 22 12.469 54.96713.372 1.00 11.33 6 C ATOM 156 CB LEU A 22 12.651 53.463 13.095 1.0011.29 6 C ATOM 157 CG LEU A 22 13.490 53.153 11.855 1.00 12.08 6 C ATOM158 CD1 LEU A 22 13.725 51.647 11.748 1.00 14.54 6 C ATOM 159 CD2 LEU A22 12.857 53.652 10.579 1.00 11.38 6 C ATOM 160 C LEU A 22 11.460 55.10914.514 1.00 11.56 6 C ATOM 161 O LEU A 22 10.270 55.364 14.275 1.0010.86 8 O ATOM 162 N LEU A 23 11.918 54.921 15.746 1.00 13.00 7 N ATOM163 CA LEU A 23 11.054 55.014 16.917 1.00 16.61 6 C ATOM 164 CB LEU A 2311.801 54.718 18.209 1.00 16.55 6 C ATOM 165 CG LEU A 23 11.858 53.26418.610 1.00 18.39 6 C ATOM 166 CD1 LEU A 23 12.928 53.050 19.675 1.0016.32 6 C ATOM 167 CD2 LEU A 23 10.501 52.804 19.142 1.00 17.70 6 C ATOM168 C LEU A 23 10.404 56.393 17.064 1.00 15.89 6 C ATOM 169 O LEU A 239.282 56.440 17.556 1.00 17.60 8 O ATOM 170 N ALA A 24 10.998 57.44016.551 1.00 16.01 7 N ATOM 171 CA ALA A 24 10.422 58.773 16.532 1.0018.43 6 C ATOM 172 CB ALA A 24 11.533 59.775 16.231 1.00 16.56 6 C ATOM173 C ALA A 24 9.330 58.951 15.481 1.00 18.75 6 C ATOM 174 O ALA A 248.778 60.058 15.427 1.00 19.41 8 O ATOM 175 N MET A 25 9.188 58.03914.512 1.00 15.42 7 N ATOM 176 CA MET A 25 8.189 58.202 13.466 1.0015.12 6 C ATOM 177 CB MET A 25 8.570 57.409 12.208 1.00 14.61 6 C ATOM178 CG MET A 25 9.787 57.989 11.504 1.00 16.53 6 C ATOM 179 SD MET A 2510.471 56.816 10.317 1.00 15.91 16 S ATOM 180 CE MET A 25 12.118 57.57610.131 1.00 18.16 6 C ATOM 181 C MET A 25 6.845 57.715 13.992 1.00 15.096 C ATOM 182 O MET A 25 6.801 56.666 14.654 1.00 15.91 8 O ATOM 183 NPRO A 26 5.757 58.441 13.743 1.00 14.86 7 N ATOM 184 CD PRO A 26 5.75559.703 12.955 1.00 14.19 6 C ATOM 185 CA PRO A 26 4.455 58.083 14.2421.00 14.29 6 C ATOM 186 CB PRO A 26 3.504 59.246 13.796 1.00 15.81 6 CATOM 187 CG PRO A 26 4.265 59.970 12.737 1.00 16.25 6 C ATOM 188 C PRO A26 3.900 56.773 13.810 1.00 13.72 6 C ATOM 189 O PRO A 26 3.192 56.13014.625 1.00 14.68 8 O ATOM 190 N GLU A 27 4.291 56.227 12.645 1.00 13.347 N ATOM 191 CA GLU A 27 3.806 54.923 12.235 1.00 13.47 6 C ATOM 192 CBGLU A 27 4.091 54.625 10.760 1.00 17.16 6 C ATOM 193 CG GLU A 27 3.99555.541 9.606 1.00 21.17 6 C ATOM 194 CD GLU A 27 4.560 56.955 9.586 1.0023.93 6 C ATOM 195 OE1 GLU A 27 5.418 57.453 10.335 1.00 20.18 8 O ATOM196 OE2 GLU A 27 4.072 57.616 8.610 1.00 25.99 8 O ATOM 197 C GLU A 274.444 53.764 13.004 1.00 10.60 6 C ATOM 198 O GLU A 27 3.954 52.64413.011 1.00 7.90 8 O ATOM 199 N VAL A 28 5.617 53.989 13.561 1.00 9.98 7N ATOM 200 CA VAL A 28 6.394 52.946 14.202 1.00 8.91 6 C ATOM 201 CB VALA 28 7.896 53.306 14.215 1.00 8.00 6 C ATOM 202 CG1 VAL A 28 8.68352.277 15.029 1.00 6.92 6 C ATOM 203 CG2 VAL A 28 8.442 53.420 12.7911.00 6.99 6 C ATOM 204 C VAL A 28 5.878 52.729 15.620 1.00 10.15 6 CATOM 205 O VAL A 28 5.925 53.618 16.428 1.00 10.34 8 O ATOM 206 N LYS A29 5.411 51.543 15.933 1.00 10.04 7 N ATOM 207 CA LYS A 29 4.865 51.23817.225 1.00 12.44 6 C ATOM 208 CB LYS A 29 3.643 50.303 17.106 1.0013.57 6 C ATOM 209 CG LYS A 29 2.432 50.906 16.421 1.00 14.50 6 C ATOM210 CD LYS A 29 2.302 52.410 16.610 1.00 15.93 6 C ATOM 211 CE LYS A 291.142 52.994 15.832 1.00 20.88 6 C ATOM 212 NZ LYS A 29 1.224 54.49715.684 1.00 22.58 7 N ATOM 213 C LYS A 29 5.891 50.550 18.129 1.00 12.916 C ATOM 214 O LYS A 29 5.769 50.754 19.332 1.00 11.55 8 O ATOM 215 NGLU A 30 6.810 49.746 17.592 1.00 10.53 7 N ATOM 216 CA GLU A 30 7.78549.053 18.424 1.00 11.26 6 C ATOM 217 CB GLU A 30 7.350 47.633 18.8311.00 13.54 6 C ATOM 218 CG GLU A 30 6.072 47.456 19.598 1.00 16.71 6 CATOM 219 CD GLU A 30 5.654 46.018 19.905 1.00 18.00 6 C ATOM 220 OE1 GLUA 30 4.403 45.814 19.971 1.00 17.65 8 O ATOM 221 OE2 GLU A 30 6.55445.183 20.077 1.00 15.08 8 O ATOM 222 C GLU A 30 9.025 48.844 17.5221.00 8.70 6 C ATOM 223 O GLU A 30 8.821 48.760 16.305 1.00 6.20 8 O ATOM224 N ALA A 31 10.198 48.728 18.108 1.00 8.38 7 N ATOM 225 CA ALA A 3111.417 48.529 17.293 1.00 5.18 6 C ATOM 226 CB ALA A 31 11.894 49.86216.752 1.00 7.29 6 C ATOM 227 C ALA A 31 12.462 47.902 18.163 1.00 9.366 C ATOM 228 O ALA A 31 12.517 48.224 19.344 1.00 9.20 8 O ATOM 229 NTYR A 32 13.162 46.872 17.670 1.00 6.49 7 N ATOM 230 CA TYR A 32 14.16646.191 18.428 1.00 8.44 6 C ATOM 231 CB TYR A 32 13.698 44.827 18.9381.00 6.51 6 C ATOM 232 CG TYR A 32 12.632 44.881 20.030 1.00 7.98 6 CATOM 233 CD1 TYR A 32 12.981 44.818 21.371 1.00 9.50 6 C ATOM 234 CE1TYR A 32 12.017 44.881 22.369 1.00 12.01 6 C ATOM 235 CD2 TYR A 3211.293 45.016 19.698 1.00 8.48 6 C ATOM 236 CE2 TYR A 32 10.324 45.07920.666 1.00 9.99 6 C ATOM 237 CZ TYR A 32 10.685 44.997 21.998 1.0011.97 6 C ATOM 238 OH TYR A 32 9.698 45.024 22.951 1.00 12.74 8 O ATOM239 C TYR A 32 15.396 45.801 17.567 1.00 8.57 6 C ATOM 240 O TYR A 3215.211 45.300 16.462 1.00 7.72 8 O ATOM 241 N VAL A 33 16.568 45.98518.158 1.00 7.80 7 N ATOM 242 CA VAL A 33 17.785 45.424 17.605 1.00 6.396 C ATOM 243 CB VAL A 33 19.048 46.137 18.150 1.00 8.75 6 C ATOM 244 CG1VAL A 33 20.273 45.355 17.658 1.00 8.40 6 C ATOM 245 CG2 VAL A 33 19.02147.589 17.720 1.00 7.33 6 C ATOM 246 C VAL A 33 17.812 43.939 17.8961.00 5.76 6 C ATOM 247 O VAL A 33 17.615 43.509 19.054 1.00 7.13 8 OATOM 248 N VAL A 34 18.079 43.073 16.894 1.00 4.78 7 N ATOM 249 CA VAL A34 18.004 41.632 17.161 1.00 6.41 6 C ATOM 250 CB VAL A 34 16.731 41.01416.512 1.00 6.60 6 C ATOM 251 CG1 VAL A 34 15.430 41.454 17.214 1.005.42 6 C ATOM 252 CG2 VAL A 34 16.583 41.384 15.032 1.00 6.47 6 C ATOM253 C VAL A 34 19.219 40.873 16.622 1.00 8.92 6 C ATOM 254 O VAL A 3419.953 41.359 15.772 1.00 10.44 8 O ATOM 255 N TYR A 35 19.405 39.62116.980 1.00 10.34 7 N ATOM 256 CA TYR A 35 20.429 38.735 16.482 1.0010.98 6 C ATOM 257 CB TYR A 35 20.884 37.774 17.543 1.00 11.65 6 C ATOM258 CG TYR A 35 21.645 38.403 18.665 1.00 14.04 6 C ATOM 259 CD1 TYR A35 22.970 38.793 18.465 1.00 17.64 6 C ATOM 260 CE1 TYR A 35 23.71239.294 19.521 1.00 18.93 6 C ATOM 261 CD2 TYR A 35 21.070 38.581 19.9051.00 15.53 6 C ATOM 262 CE2 TYR A 35 21.807 39.100 20.951 1.00 16.48 6 CATOM 263 CZ TYR A 35 23.122 39.436 20.762 1.00 19.05 6 C ATOM 264 OH TYRA 35 23.870 39.944 21.812 1.00 20.64 8 O ATOM 265 C TYR A 35 19.81537.881 15.371 1.00 11.75 6 C ATOM 266 O TYR A 35 18.624 37.596 15.4811.00 14.45 8 O ATOM 267 N GLY A 36 20.585 37.514 14.415 1.00 12.14 7 NATOM 268 CA GLY A 36 20.085 36.727 13.250 1.00 15.97 6 C ATOM 269 C GLYA 36 20.689 37.429 12.036 1.00 16.50 6 C ATOM 270 O GLY A 36 21.41838.441 12.153 1.00 21.32 8 O ATOM 271 N GLY A 37 20.171 37.205 10.8831.00 20.97 7 N ATOM 272 CA GLU A 37 20.478 37.746 9.563 1.00 24.00 6 CATOM 273 CB GLU A 37 19.730 36.869 8.521 1.00 28.29 6 C ATOM 274 CG GLUA 37 19.992 37.381 7.113 1.00 31.96 6 C ATOM 275 CD GLU A 37 19.03436.898 6.059 1.00 35.65 6 C ATOM 276 OE1 GLU A 37 18.268 35.905 6.2131.00 37.44 8 O ATOM 277 OE2 GLU A 37 19.114 37.582 5.011 1.00 38.02 8 OATOM 278 C GLU A 37 19.912 39.147 9.418 1.00 23.50 6 C ATOM 279 O GLU A37 20.409 40.033 8.717 1.00 24.72 8 O ATOM 280 N TYR A 38 18.815 39.38210.151 1.00 21.31 7 N ATOM 281 CA TYR A 38 18.292 40.761 10.215 1.0018.05 6 C ATOM 282 CB TYR A 38 16.758 40.730 10.202 1.00 18.36 6 C ATOM283 CG TYR A 38 16.285 40.221 8.856 1.00 21.89 6 C ATOM 284 CD1 TYR A 3816.438 41.026 7.736 1.00 23.00 6 C ATOM 285 CE1 TYR A 38 15.971 40.5906.514 1.00 25.96 6 C ATOM 286 CD2 TYR A 38 15.624 39.002 8.750 1.0024.51 6 C ATOM 287 CE2 TYR A 38 15.160 38.565 7.520 1.00 26.07 6 C ATOM288 CZ TYR A 38 15.410 39.342 6.414 1.00 25.27 6 C ATOM 289 OH TYR A 3814.955 38.933 5.173 1.00 29.39 8 O ATOM 290 C TYR A 38 18.810 41.33611.545 1.00 15.22 6 C ATOM 291 O TYR A 38 18.883 40.635 12.546 1.0016.00 8 O ATOM 292 N ASP A 39 18.955 42.613 11.599 1.00 14.06 7 N ATOM293 CA ASP A 39 19.565 43.412 12.643 1.00 13.19 6 C ATOM 294 CB ASP A 3920.378 44.510 11.873 1.00 11.78 6 C ATOM 295 CG ASP A 39 21.527 43.89111.130 1.00 13.09 6 C ATOM 296 OD1 ASP A 39 21.795 42.747 11.415 1.0012.32 8 O ATOM 297 OD2 ASP A 39 22.173 44.518 10.302 1.00 16.95 8 O ATOM298 C ASP A 39 18.532 44.178 13.447 1.00 10.56 6 C ATOM 299 O ASP A 3918.763 44.513 14.618 1.00 8.22 8 O ATOM 300 N LEU A 40 17.434 44.49612.735 1.00 9.46 7 N ATOM 301 CA LEU A 40 16.344 45.250 13.355 1.00 9.226 C ATOM 302 CB LEU A 40 16.246 46.683 12.865 1.00 12.49 6 C ATOM 303 CGLEU A 40 17.180 47.814 13.176 1.00 14.92 6 C ATOM 304 CD1 LEU A 4016.680 49.055 12.461 1.00 15.41 6 C ATOM 305 CD2 LEU A 40 17.274 48.04914.672 1.00 17.24 6 C ATOM 306 C LEU A 40 14.966 44.704 12.880 1.00 8.696 C ATOM 307 O LEU A 40 14.829 44.337 11.705 1.00 6.37 8 O ATOM 308 NILE A 41 14.001 44.818 13.778 1.00 7.55 7 N ATOM 309 CA ILE A 41 12.59844.417 13.498 1.00 8.63 6 C ATOM 310 CB ILE A 41 12.087 43.119 14.0761.00 9.76 6 C ATOM 311 CG2 ILE A 41 12.326 42.903 15.566 1.00 11.62 6 CATOM 312 CG1 ILE A 41 10.575 42.937 13.807 1.00 10.77 6 C ATOM 313 CD1ILE A 41 10.193 41.468 13.867 1.00 13.08 6 C ATOM 314 C ILE A 41 11.75045.593 13.967 1.00 10.12 6 C ATOM 315 O ILE A 41 12.005 46.153 15.0531.00 7.54 8 O ATOM 316 N VAL A 42 10.953 46.090 13.043 1.00 8.66 7 NATOM 317 CA VAL A 42 10.139 47.268 13.395 1.00 10.64 6 C ATOM 318 CB VALA 42 10.656 48.463 12.600 1.00 14.70 6 C ATOM 319 CG1 VAL A 42 9.70549.597 12.514 1.00 14.15 6 C ATOM 320 CG2 VAL A 42 11.929 49.038 13.2951.00 17.64 6 C ATOM 321 C VAL A 42 8.676 46.957 13.114 1.00 9.69 6 CATOM 322 O VAL A 42 8.404 46.277 12.123 1.00 9.29 8 O ATOM 323 N LYS A43 7.785 47.249 14.067 1.00 9.12 7 N ATOM 324 CA LYS A 43 6.352 47.04313.919 1.00 7.52 6 C ATOM 325 CB LYS A 43 5.757 46.570 15.274 1.00 8.786 C ATOM 326 CG LYS A 43 4.246 46.290 15.145 1.00 12.03 6 C ATOM 327 CDLYS A 43 3.692 45.912 16.541 1.00 11.71 6 C ATOM 328 CE LYS A 43 2.20045.790 16.524 1.00 13.09 6 C ATOM 329 NZ LYS A 43 1.593 45.210 17.7481.00 11.39 7 N ATOM 330 C LYS A 43 5.697 48.367 13.564 1.00 7.31 6 CATOM 331 O LYS A 43 5.845 49.413 14.216 1.00 6.38 8 O ATOM 332 N VAL A44 4.971 48.402 12.451 1.00 6.74 7 N ATOM 333 CA VAL A 44 4.336 49.58511.930 1.00 7.54 6 C ATOM 334 CB VAL A 44 4.898 50.065 10.573 1.00 11.016 C ATOM 335 CG1 VAL A 44 6.434 50.232 10.650 1.00 12.68 6 C ATOM 336CG2 VAL A 44 4.541 49.098 9.469 1.00 10.39 6 C ATOM 337 C VAL A 44 2.82549.371 11.758 1.00 9.21 6 C ATOM 338 O VAL A 44 2.292 48.264 11.561 1.008.93 8 O ATOM 339 N GLU A 45 2.134 50.493 11.957 1.00 10.36 7 N ATOM 340CA GLU A 45 0.665 50.496 11.858 1.00 12.06 6 C ATOM 341 CB GLU A 450.004 50.496 13.238 1.00 12.25 6 C ATOM 342 CG GLU A 45 0.012 49.08613.798 1.00 15.23 6 C ATOM 343 CD GLU A 45 −0.408 48.927 15.233 1.0018.39 6 C ATOM 344 OE1 GLU A 45 −0.169 47.876 15.848 1.00 21.70 8 O ATOM345 OE2 GLU A 45 −1.021 49.852 15.769 1.00 21.18 8 O ATOM 346 C GLU A 450.236 51.698 11.047 1.00 10.21 6 C ATOM 347 O GLU A 45 0.783 52.78011.208 1.00 10.74 8 O ATOM 348 N THR A 46 −0.422 51.431 9.912 1.00 10.027 N ATOM 349 CA THR A 46 −0.794 52.546 9.056 1.00 10.52 6 C ATOM 350 CBTHR A 46 −0.030 52.589 7.701 1.00 12.10 6 C ATOM 351 OG1 THR A 46 −0.28051.395 6.931 1.00 9.65 8 O ATOM 352 CG2 THR A 46 1.482 52.648 7.918 1.0014.04 6 C ATOM 353 C THR A 46 −2.297 52.431 8.783 1.00 10.40 6 C ATOM354 O THR A 46 −2.889 51.353 8.880 1.00 6.84 8 O ATOM 355 N ASP A 47−2.838 53.524 8.240 1.00 10.42 7 N ATOM 356 CA ASP A 47 −4.298 53.4277.930 1.00 13.28 6 C ATOM 357 CB ASP A 47 −4.956 54.763 7.696 1.00 17.796 C ATOM 358 CG ASP A 47 −4.912 55.703 8.873 1.00 22.85 6 C ATOM 359 OD1ASP A 47 −5.187 55.282 10.024 1.00 24.70 8 O ATOM 360 OD2 ASP A 47−4.546 56.871 8.607 1.00 26.41 8 O ATOM 361 C ASP A 47 −4.556 52.5256.736 1.00 12.26 6 C ATOM 362 O ASP A 47 −5.638 51.891 6.708 1.00 9.75 8O ATOM 363 N THR A 48 −3.726 52.630 5.681 1.00 10.33 7 N ATOM 364 CA THRA 48 −3.998 51.918 4.451 1.00 10.32 6 C ATOM 365 CB THR A 48 −4.55352.795 3.306 1.00 12.96 6 C ATOM 366 OG1 THR A 48 −3.520 53.774 3.0201.00 11.60 8 O ATOM 367 CG2 THR A 48 −5.883 53.503 3.608 1.00 15.12 6 CATOM 368 C THR A 48 −2.701 51.285 3.893 1.00 11.08 6 C ATOM 369 O THR A48 −1.596 51.629 4.290 1.00 10.17 8 O ATOM 370 N LEU A 49 −2.831 50.3263.009 1.00 11.90 7 N ATOM 371 CA LEU A 49 −1.700 49.679 2.357 1.00 13.326 C ATOM 372 CB LEU A 49 −2.134 48.604 1.395 1.00 14.57 6 C ATOM 373 CGLEU A 49 −1.094 47.810 0.623 1.00 17.35 6 C ATOM 374 CD1 LEU A 49 −0.05647.181 1.546 1.00 15.04 6 C ATOM 375 CD2 LEU A 49 −1.808 46.682 −0.1411.00 17.00 6 C ATOM 376 C LEU A 49 −0.852 50.701 1.596 1.00 14.01 6 CATOM 377 O LEU A 49 0.391 50.684 1.581 1.00 13.28 8 O ATOM 378 N LYS A50 −1.512 51.662 0.995 1.00 14.48 7 N ATOM 379 CA LYS A 50 −0.901 52.7710.305 1.00 17.54 6 C ATOM 380 CB LYS A 50 −2.009 53.660 −0.266 1.0021.43 6 C ATOM 381 CG LYS A 50 −1.525 54.829 −1.120 1.00 27.31 6 C ATOM382 CD LYS A 50 −2.665 55.246 −2.068 1.00 30.92 6 C ATOM 383 CE LYS A 50−2.165 55.936 −3.327 1.00 35.13 6 C ATOM 384 NZ LYS A 50 −1.681 55.003−4.385 1.00 37.07 7 N ATOM 385 C LYS A 50 −0.042 53.546 1.285 1.00 15.326 C ATOM 386 O LYS A 50 1.063 53.914 0.938 1.00 14.61 8 O ATOM 387 N ASPA 51 −0.491 53.838 2.496 1.00 14.52 7 N ATOM 388 CA ASP A 51 0.31454.507 3.504 1.00 13.63 6 C ATOM 389 CB ASP A 51 −0.439 54.839 4.7751.00 16.05 6 C ATOM 390 CG ASP A 51 −1.564 55.875 4.586 1.00 19.66 6 CATOM 391 OD1 ASP A 51 −1.389 56.778 3.763 1.00 18.33 8 O ATOM 392 OD2ASP A 51 −2.609 55.712 5.280 1.00 20.80 8 O ATOM 393 C ASP A 51 1.56353.630 3.824 1.00 12.48 6 C ATOM 394 O ASP A 51 2.638 54.196 4.026 1.0010.69 8 O ATOM 395 N LEU A 52 1.429 52.318 3.906 1.00 10.26 7 N ATOM 396CA LEU A 52 2.587 51.461 4.209 1.00 10.76 6 C ATOM 397 CB LEU A 52 2.16150.001 4.439 1.00 8.04 6 C ATOM 398 CG LEU A 52 3.303 48.994 4.717 1.008.93 6 C ATOM 399 CD1 LEU A 52 4.036 49.344 5.991 1.00 8.54 6 C ATOM 400CD2 LEU A 52 2.727 47.582 4.789 1.00 6.69 6 C ATOM 401 C LEU A 52 3.60951.536 3.086 1.00 10.23 6 C ATOM 402 O LEU A 52 4.803 51.700 3.307 1.0010.81 8 O ATOM 403 N ASP A 53 3.139 51.505 1.847 1.00 12.35 7 N ATOM 404CA ASP A 53 3.945 51.675 0.658 1.00 14.81 6 C ATOM 405 CB ASP A 53 3.00051.762 −0.539 1.00 16.01 6 C ATOM 406 CG ASP A 53 2.380 50.470 −0.9811.00 16.50 6 C ATOM 407 OD1 ASP A 53 2.487 49.314 −0.542 1.00 16.66 8 OATOM 408 OD2 ASP A 53 1.600 50.652 −1.934 1.00 19.89 8 O ATOM 409 C ASPA 53 4.760 52.960 0.728 1.00 16.37 6 C ATOM 410 O ASP A 53 5.984 52.9760.478 1.00 13.89 8 O ATOM 411 N GLN A 54 4.067 54.021 1.166 1.00 16.03 7N ATOM 412 CA GLN A 54 4.736 55.341 1.262 1.00 17.59 6 C ATOM 413 CB GLNA 54 3.640 56.376 1.250 1.00 22.16 6 C ATOM 414 CG GLN A 54 3.822 57.8481.338 1.00 30.35 6 C ATOM 415 CD GLN A 54 2.485 58.612 1.418 1.00 35.566 C ATOM 416 OE1 GLN A 54 1.366 58.133 1.134 1.00 37.13 8 O ATOM 417 NE2GLN A 54 2.602 59.867 1.844 1.00 36.35 7 N ATOM 418 C GLN A 54 5.72255.402 2.394 1.00 15.60 6 C ATOM 419 O GLN A 54 6.791 56.008 2.287 1.0016.58 8 O ATOM 420 N PHE A 55 5.539 54.709 3.507 1.00 14.54 7 N ATOM 421CA PHE A 55 6.508 54.584 4.590 1.00 12.24 6 C ATOM 422 CB PHE A 55 5.90153.747 5.733 1.00 12.77 6 C ATOM 423 CG PHE A 55 6.785 53.705 6.955 1.0011.74 6 C ATOM 424 CD1 PHE A 55 6.962 54.849 7.699 1.00 13.70 6 C ATOM425 CD2 PHE A 55 7.483 52.560 7.281 1.00 11.58 6 C ATOM 426 CE1 PHE A 557.786 54.861 8.806 1.00 11.72 6 C ATOM 427 CE2 PHE A 55 8.345 52.5648.372 1.00 13.77 6 C ATOM 428 CZ PHE A 55 8.485 53.705 9.134 1.00 13.936 C ATOM 429 C PHE A 55 7.793 53.901 4.107 1.00 11.18 6 C ATOM 430 O PHEA 55 8.914 54.384 4.344 1.00 11.64 8 O ATOM 431 N ILE A 56 7.694 52.7783.413 1.00 9.30 7 N ATOM 432 CA ILE A 56 8.772 52.040 2.820 1.00 10.44 6C ATOM 433 CB ILE A 56 8.350 50.766 2.043 1.00 12.03 6 C ATOM 434 CG2ILE A 56 9.595 50.037 1.526 1.00 11.62 6 C ATOM 435 CG1 ILE A 56 7.50749.761 2.823 1.00 13.57 6 C ATOM 436 CD1 ILE A 56 8.161 49.397 4.1411.00 16.82 6 C ATOM 437 C ILE A 56 9.521 52.936 1.813 1.00 10.94 6 CATOM 438 O ILE A 56 10.737 53.141 2.008 1.00 10.83 8 O ATOM 439 N THR A57 8.820 53.526 0.843 1.00 10.32 7 N ATOM 440 CA THR A 57 9.547 54.292−0.177 1.00 15.45 6 C ATOM 441 CB THR A 57 8.786 54.444 −1.513 1.0015.05 6 C ATOM 442 OG1 THR A 57 7.636 55.254 −1.248 1.00 17.59 8 O ATOM443 CG2 THR A 57 8.341 53.042 −1.938 1.00 15.90 6 C ATOM 444 C THR A 5710.046 55.637 0.282 1.00 18.09 6 C ATOM 445 O THR A 57 11.145 56.074−0.104 1.00 17.72 8 O ATOM 446 N GLU A 58 9.248 56.355 1.064 1.00 18.707 N ATOM 447 CA GLU A 58 9.648 57.717 1.436 1.00 21.41 6 C ATOM 448 CBGLU A 58 8.422 58.659 1.495 1.00 23.68 6 C ATOM 449 CG GLU A 58 7.66858.633 0.164 1.00 28.38 6 C ATOM 450 CD GLU A 58 8.510 58.884 −1.0831.00 32.18 6 C ATOM 451 OE1 GLU A 58 9.164 59.955 −1.157 1.00 34.50 8 OATOM 452 OE2 GLU A 58 8.542 58.044 −2.033 1.00 31.90 8 O ATOM 453 C GLUA 58 10.458 57.767 2.715 1.00 20.49 6 C ATOM 454 O GLU A 58 11.15858.771 2.852 1.00 19.41 8 O ATOM 455 N LYS A 59 10.333 56.793 3.610 1.0017.03 7 N ATOM 456 CA LYS A 59 11.082 56.882 4.860 1.00 18.11 6 C ATOM457 CB LYS A 59 10.157 56.735 6.085 1.00 20.27 6 C ATOM 458 CG LYS A 599.242 57.974 6.156 1.00 25.90 6 C ATOM 459 CD LYS A 59 8.467 58.1037.454 1.00 31.51 6 C ATOM 460 CE LYS A 59 7.799 59.461 7.620 1.00 33.056 C ATOM 461 NZ LYS A 59 6.928 59.580 8.833 1.00 33.55 7 N ATOM 462 CLYS A 59 12.227 55.877 4.872 1.00 16.26 6 C ATOM 463 O LYS A 59 13.41156.261 4.886 1.00 14.64 8 O ATOM 464 N ILE A 60 11.861 54.602 4.787 1.0013.46 7 N ATOM 465 CA ILE A 60 12.891 53.543 4.870 1.00 12.61 6 C ATOM466 CB ILE A 60 12.205 52.176 4.758 1.00 12.63 6 C ATOM 467 CG2 ILE A 6013.152 50.999 4.855 1.00 14.47 6 C ATOM 468 CG1 ILE A 60 11.078 52.0455.811 1.00 13.49 6 C ATOM 469 CD1 ILE A 60 11.557 52.160 7.226 1.0015.68 6 C ATOM 470 C ILE A 60 13.933 53.683 3.769 1.00 12.10 6 C ATOM471 O ILE A 60 15.130 53.686 4.030 1.00 11.15 8 O ATOM 472 N ARG A 6113.477 53.685 2.531 1.00 11.05 7 N ATOM 473 CA ARG A 61 14.398 53.7021.390 1.00 14.35 6 C ATOM 474 CB ARG A 61 13.613 53.280 0.121 1.00 12.926 C ATOM 475 CG ARG A 61 13.408 51.765 0.293 1.00 15.09 6 C ATOM 476 CDARG A 61 12.740 51.100 −0.894 1.00 14.74 6 C ATOM 477 NE ARG A 61 12.69449.638 −0.750 1.00 10.39 7 N ATOM 478 CZ ARG A 61 12.155 48.865 −1.6831.00 10.84 6 C ATOM 479 NH1 ARG A 61 11.705 49.379 −2.843 1.00 8.51 7 NATOM 480 NH2 ARG A 61 12.149 47.541 −1.520 1.00 7.77 7 N ATOM 481 C ARGA 61 15.230 54.952 1.199 1.00 12.68 6 C ATOM 482 O ARG A 61 16.12654.985 0.379 1.00 12.57 8 O ATOM 483 N LYS A 62 14.965 56.045 1.874 1.0011.92 7 N ATOM 484 CA LYS A 62 15.767 57.215 1.937 1.00 15.99 6 C ATOM485 CB LYS A 62 14.866 58.469 2.069 1.00 18.60 6 C ATOM 486 CG LYS A 6214.165 58.660 0.733 1.00 21.84 6 C ATOM 487 CD LYS A 62 13.529 60.0190.531 1.00 25.52 6 C ATOM 488 CE LYS A 62 12.694 59.946 −0.762 1.0025.02 6 C ATOM 489 NZ LYS A 62 12.156 61.305 −1.045 1.00 27.48 7 N ATOM490 C LYS A 62 16.820 57.155 3.057 1.00 17.44 6 C ATOM 491 O LYS A 6217.554 58.146 3.135 1.00 17.35 8 O ATOM 492 N MET A 63 16.892 56.1173.867 1.00 14.00 7 N ATOM 493 CA MET A 63 17.923 56.000 4.889 1.00 16.246 C ATOM 494 CB MET A 63 17.312 55.433 6.183 1.00 17.88 6 C ATOM 495 CGMET A 63 16.270 56.338 6.811 1.00 21.60 6 C ATOM 496 SD MET A 63 15.47055.691 8.306 1.00 20.40 16 S ATOM 497 CE MET A 63 14.362 54.509 7.5981.00 24.10 6 C ATOM 498 C MET A 63 19.041 55.086 4.400 1.00 14.86 6 CATOM 499 O MET A 63 18.889 53.879 4.279 1.00 11.39 8 O ATOM 500 N PRO A64 20.235 55.621 4.123 1.00 16.04 7 N ATOM 501 CD PRO A 64 20.544 57.0764.204 1.00 16.05 6 C ATOM 502 CA PRO A 64 21.320 54.831 3.549 1.00 16.256 C ATOM 503 CB PRO A 64 22.349 55.886 3.119 1.00 17.65 6 C ATOM 504 CGPRO A 64 22.041 57.059 4.001 1.00 18.37 6 C ATOM 505 C PRO A 64 21.89253.725 4.391 1.00 16.20 6 C ATOM 506 O PRO A 64 22.415 52.724 3.846 1.0017.51 8 O ATOM 507 N GLU A 65 21.774 53.746 5.713 1.00 16.16 7 N ATOM508 CA GLU A 65 22.214 52.697 6.620 1.00 19.24 6 C ATOM 509 CB GLU A 6522.275 53.230 8.060 1.00 21.22 6 C ATOM 510 CG GLU A 65 22.899 54.6168.078 1.00 25.48 6 C ATOM 511 CD GLU A 65 21.970 55.758 7.731 1.00 30.116 C ATOM 512 OE1 GLU A 65 20.719 55.794 7.576 1.00 26.69 8 O ATOM 513OE2 GLU A 65 22.562 56.925 7.697 1.00 35.08 8 O ATOM 514 C GLU A 6521.322 51.462 6.576 1.00 18.67 6 C ATOM 515 O GLU A 65 21.812 50.3376.859 1.00 17.95 8 O ATOM 516 N ILE A 66 20.094 51.634 6.036 1.00 15.767 N ATOM 517 CA ILE A 66 19.264 50.426 5.886 1.00 15.12 6 C ATOM 518 CBILE A 66 17.761 50.790 6.040 1.00 15.29 6 C ATOM 519 CG2 ILE A 66 16.98549.503 5.763 1.00 17.90 6 C ATOM 520 CG1 ILE A 66 17.536 51.382 7.4031.00 15.51 6 C ATOM 521 CD1 ILE A 66 16.130 51.794 7.783 1.00 18.15 6 CATOM 522 C ILE A 66 19.547 49.790 4.557 1.00 15.35 6 C ATOM 523 O ILE A66 19.500 50.521 3.558 1.00 16.62 8 O ATOM 524 N GLN A 67 19.964 48.5534.453 1.00 17.21 7 N ATOM 525 CA GLN A 67 20.439 47.860 3.282 1.00 19.066 C ATOM 526 CB GLN A 67 21.783 47.155 3.681 1.00 22.67 6 C ATOM 527 CGGLN A 67 22.821 48.236 4.024 1.00 27.17 6 C ATOM 528 CD GLN A 67 24.17247.665 4.393 1.00 29.47 6 C ATOM 529 OE1 GLN A 67 25.008 48.411 4.9161.00 30.95 8 O ATOM 530 NE2 GLN A 67 24.408 46.398 4.137 1.00 28.71 7 NATOM 531 C GLN A 67 19.599 46.747 2.673 1.00 20.41 6 C ATOM 532 O GLN A67 19.769 46.389 1.495 1.00 21.52 8 O ATOM 533 N MET A 68 18.877 45.9483.425 1.00 18.04 7 N ATOM 534 CA MET A 68 17.961 44.941 2.966 1.00 16.996 C ATOM 535 CB MET A 68 18.584 43.560 2.923 1.00 19.00 6 C ATOM 536 CGMET A 68 18.006 42.708 1.809 1.00 25.20 6 C ATOM 537 SD MET A 68 18.50740.980 1.906 1.00 32.29 16 S ATOM 538 CE MET A 68 20.191 41.033 1.2691.00 31.84 6 C ATOM 539 C MET A 68 16.753 44.876 3.930 1.00 14.82 6 CATOM 540 O MET A 68 16.955 44.821 5.156 1.00 9.54 8 O ATOM 541 N THR A69 15.567 44.888 3.325 1.00 10.34 7 N ATOM 542 CA THR A 69 14.343 44.8244.099 1.00 11.13 6 C ATOM 543 CB THR A 69 13.513 46.151 4.085 1.00 11.226 C ATOM 544 OG1 THR A 69 13.145 46.401 2.740 1.00 11.52 8 O ATOM 545CG2 THR A 69 14.291 47.328 4.617 1.00 10.08 6 C ATOM 546 C THR A 6913.428 43.733 3.590 1.00 12.72 6 C ATOM 547 O THR A 69 13.574 43.3102.431 1.00 12.83 8 O ATOM 548 N SER A 70 12.650 43.124 4.475 1.00 10.147 N ATOM 549 CA SER A 70 11.693 42.093 4.182 1.00 8.64 6 C ATOM 550 CBSER A 70 12.094 40.688 4.647 1.00 8.17 6 C ATOM 551 OG SER A 70 11.03939.830 4.251 1.00 11.27 8 O ATOM 552 C SER A 70 10.464 42.513 5.055 1.0012.16 6 C ATOM 553 O SER A 70 10.659 42.602 6.288 1.00 9.70 8 O ATOM 554N THR A 71 9.352 42.753 4.380 1.00 10.49 7 N ATOM 555 CA THR A 71 8.15343.198 5.110 1.00 11.09 6 C ATOM 556 CB THR A 71 7.587 44.481 4.499 1.0011.77 6 C ATOM 557 OG1 THR A 71 8.593 45.476 4.690 1.00 10.42 8 O ATOM558 CG2 THR A 71 6.295 45.014 5.189 1.00 13.37 6 C ATOM 559 C THR A 717.092 42.123 5.214 1.00 10.51 6 C ATOM 560 O THR A 71 6.771 41.472 4.2171.00 10.60 8 O ATOM 561 N MET A 72 6.591 41.918 6.423 1.00 8.76 7 N ATOM562 CA MET A 72 5.484 40.937 6.575 1.00 11.11 6 C ATOM 563 CB MET A 725.816 39.916 7.651 1.00 13.22 6 C ATOM 564 CG MET A 72 6.324 38.6266.987 1.00 21.65 6 C ATOM 565 SD MET A 72 7.682 37.932 7.913 1.00 27.1316 S ATOM 566 CE MET A 72 9.041 38.835 7.191 1.00 25.46 6 C ATOM 567 CMET A 72 4.216 41.662 7.019 1.00 10.65 6 C ATOM 568 O MET A 72 4.24742.322 8.052 1.00 8.92 8 O ATOM 569 N ILE A 73 3.140 41.593 6.255 1.0010.57 7 N ATOM 570 CA ILE A 73 1.905 42.309 6.581 1.00 8.83 6 C ATOM 571CB ILE A 73 1.359 42.913 5.279 1.00 8.76 6 C ATOM 572 CG2 ILE A 73 0.06143.700 5.504 1.00 7.15 6 C ATOM 573 CG1 ILE A 73 2.472 43.768 4.604 1.008.63 6 C ATOM 574 CD1 ILE A 73 2.028 44.206 3.186 1.00 8.44 6 C ATOM 575C ILE A 73 0.877 41.401 7.223 1.00 10.71 6 C ATOM 576 O ILE A 73 0.58240.281 6.799 1.00 12.18 8 O ATOM 577 N ALA A 74 0.305 41.845 8.320 1.0011.15 7 N ATOM 578 CA ALA A 74 −0.650 41.052 9.086 1.00 11.38 6 C ATOM579 CB ALA A 74 −0.976 41.637 10.453 1.00 8.81 6 C ATOM 580 C ALA A 74−1.900 40.883 8.226 1.00 13.75 6 C ATOM 581 O ALA A 74 −2.334 41.8847.674 1.00 9.08 8 O ATOM 582 N ILE A 75 −2.464 39.673 8.299 1.00 14.79 7N ATOM 583 CA ILE A 75 −3.640 39.439 7.473 1.00 20.01 6 C ATOM 584 CBILE A 75 −3.364 38.627 6.190 1.00 20.01 6 C ATOM 585 CG2 ILE A 75 −2.74439.438 5.070 1.00 20.74 6 C ATOM 586 CG1 ILE A 75 −2.502 37.432 6.5671.00 21.65 6 C ATOM 587 CD1 ILE A 75 −2.352 36.330 5.553 1.00 23.37 6 CATOM 588 C ILE A 75 −4.656 38.685 8.333 1.00 21.16 6 C ATOM 589 O ILE A75 −4.293 38.301 9.452 1.00 19.96 8 O ATOM 590 OT ILE A 75 −5.709 38.3257.766 1.00 24.56 8 O ATOM 591 CB VAL B 2 15.203 36.576 27.110 1.00 10.816 C ATOM 592 CG1 VAL B 2 15.489 36.311 25.630 1.00 10.60 6 C ATOM 593CG2 VAL B 2 16.487 36.270 27.887 1.00 9.64 6 C ATOM 594 C VAL B 2 13.49138.209 26.316 1.00 9.81 6 C ATOM 595 O VAL B 2 12.467 37.543 26.535 1.008.96 8 O ATOM 596 N VAL B 2 14.395 38.441 28.691 1.00 10.90 7 N ATOM 597CA VAL B 2 14.702 38.012 27.247 1.00 9.65 6 C ATOM 598 N THR B 3 13.64139.006 25.278 1.00 8.35 7 N ATOM 599 CA THR B 3 12.571 39.256 24.3181.00 7.94 6 C ATOM 600 CB THR B 3 12.241 40.729 24.045 1.00 8.58 6 CATOM 601 OG1 THR B 3 11.836 41.393 25.244 1.00 8.95 8 O ATOM 602 CG2 THRB 3 11.075 40.881 23.044 1.00 6.74 6 C ATOM 603 C THR B 3 13.026 38.66222.981 1.00 10.25 6 C ATOM 604 O THR B 3 14.248 38.762 22.725 1.00 6.938 O ATOM 605 N ALA B 4 12.186 37.851 22.328 1.00 7.68 7 N ATOM 606 CAALA B 4 12.535 37.308 21.033 1.00 8.07 6 C ATOM 607 CB ALA B 4 13.07335.859 21.152 1.00 5.87 6 C ATOM 608 C ALA B 4 11.315 37.351 20.096 1.009.20 6 C ATOM 609 O ALA B 4 10.154 37.299 20.559 1.00 9.29 8 O ATOM 610N PHE B 5 11.596 37.401 18.802 1.00 6.28 7 N ATOM 611 CA PHE B 5 10.59237.272 17.760 1.00 8.35 6 C ATOM 612 CB PHE B 5 10.612 38.420 16.7521.00 6.55 6 C ATOM 613 CG PHE B 5 10.185 39.729 17.409 1.00 7.88 6 CATOM 614 CD1 PHE B 5 11.087 40.520 18.080 1.00 6.54 6 C ATOM 615 CD2 PHEB 5 8.853 40.140 17.310 1.00 8.11 6 C ATOM 616 CE1 PHE B 5 10.653 41.71318.654 1.00 8.22 6 C ATOM 617 CE2 PHE B 5 8.436 41.346 17.873 1.00 7.716 C ATOM 618 CZ PHE B 5 9.335 42.140 18.531 1.00 7.27 6 C ATOM 619 C PHEB 5 10.793 35.963 16.987 1.00 7.00 6 C ATOM 620 O PHE B 5 11.904 35.64016.514 1.00 6.91 8 O ATOM 621 N ILE B 6 9.771 35.121 16.971 1.00 7.10 7N ATOM 622 CA ILE B 6 9.898 33.824 16.270 1.00 7.30 6 C ATOM 623 CB ILEB 6 9.190 32.706 17.057 1.00 7.52 6 C ATOM 624 CG2 ILE B 6 9.353 31.39616.284 1.00 6.04 6 C ATOM 625 CG1 ILE B 6 9.708 32.559 18.504 1.00 6.966 C ATOM 626 CD1 ILE B 6 8.811 31.481 19.176 1.00 12.29 6 C ATOM 627 CILE B 6 9.210 33.940 14.917 1.00 10.08 6 C ATOM 628 O ILE B 6 7.99134.222 14.923 1.00 10.99 8 O ATOM 629 N LEU B 7 9.882 33.668 13.819 1.0011.51 7 N ATOM 630 CA LEU B 7 9.237 33.678 12.492 1.00 12.43 6 C ATOM631 CB LEU B 7 10.173 34.267 11.447 1.00 12.72 6 C ATOM 632 CG LEU B 710.630 35.703 11.722 1.00 17.00 6 C ATOM 633 CD1 LEU B 7 11.870 36.07810.883 1.00 17.22 6 C ATOM 634 CD2 LEU B 7 9.485 36.665 11.508 1.0018.19 6 C ATOM 635 C LEU B 7 8.919 32.224 12.235 1.00 14.19 6 C ATOM 636O LEU B 7 9.746 31.328 12.500 1.00 13.47 8 O ATOM 637 N MET B 8 7.68831.875 11.846 1.00 13.99 7 N ATOM 638 CA MET B 8 7.306 30.486 11.7081.00 13.46 6 C ATOM 639 CB MET B 8 6.368 30.028 12.835 1.00 16.11 6 CATOM 640 CG MET B 8 6.691 30.264 14.271 1.00 18.68 6 C ATOM 641 SD MET B8 5.428 29.803 15.535 1.00 20.37 16 S ATOM 642 CE MET B 8 4.118 29.46814.437 1.00 17.84 6 C ATOM 643 C MET B 8 6.609 30.158 10.390 1.00 15.906 C ATOM 644 O MET B 8 5.832 30.941 9.852 1.00 14.92 8 O ATOM 645 N VALB 9 6.857 28.937 9.914 1.00 16.65 7 N ATOM 646 CA VAL B 9 6.126 28.4438.730 1.00 16.44 6 C ATOM 647 CB VAL B 9 7.072 27.868 7.674 1.00 17.96 6C ATOM 648 CG1 VAL B 9 6.361 27.078 6.558 1.00 16.89 6 C ATOM 649 CG2VAL B 9 7.856 29.021 7.048 1.00 17.27 6 C ATOM 650 C VAL B 9 5.25827.333 9.285 1.00 17.36 6 C ATOM 651 O VAL B 9 5.868 26.512 9.992 1.0018.71 8 O ATOM 652 N THR B 10 3.947 27.306 9.086 1.00 16.83 7 N ATOM 653CA THR B 10 3.149 26.199 9.609 1.00 16.97 6 C ATOM 654 CB THR B 10 1.86026.674 10.313 1.00 17.72 6 C ATOM 655 OG1 THR B 10 1.014 27.388 9.3761.00 16.78 8 O ATOM 656 CG2 THR B 10 2.219 27.659 11.422 1.00 15.85 6 CATOM 657 C THR B 10 2.690 25.289 8.460 1.00 18.72 6 C ATOM 658 O THR B10 2.941 25.560 7.279 1.00 19.05 8 O ATOM 659 N ALA B 11 2.101 24.1378.755 1.00 20.00 7 N ATOM 660 CA ALA B 11 1.480 23.354 7.657 1.00 20.046 C ATOM 661 CB ALA B 11 0.916 22.098 8.243 1.00 20.18 6 C ATOM 662 CALA B 11 0.384 24.233 7.072 1.00 22.64 6 C ATOM 663 O ALA B 11 −0.25924.987 7.818 1.00 22.86 8 O ATOM 664 N ALA B 12 0.184 24.225 5.759 1.0024.51 7 N ATOM 665 CA ALA B 12 −0.856 25.096 5.158 1.00 24.49 6 C ATOM666 CB ALA B 12 −0.865 24.883 3.649 1.00 23.68 6 C ATOM 667 C ALA B 12−2.233 24.886 5.770 1.00 22.73 6 C ATOM 668 O ALA B 12 −2.587 23.8266.290 1.00 22.75 8 O ATOM 669 N GLY B 13 −2.983 25.998 5.943 1.00 23.237 N ATOM 670 CA GLY B 13 −4.307 25.962 6.557 1.00 20.92 6 C ATOM 671 CGLY B 13 −4.310 25.750 8.059 1.00 22.76 6 C ATOM 672 O GLY B 13 −5.35825.433 8.643 1.00 23.11 8 O ATOM 673 N LYS B 14 −3.158 25.825 8.750 1.0020.91 7 N ATOM 674 CA LYS B 14 −3.162 25.571 10.173 1.00 20.15 6 C ATOM675 CB LYS B 14 −2.198 24.425 10.520 1.00 21.56 6 C ATOM 676 CG LYS B 14−2.514 23.101 9.799 1.00 23.27 6 C ATOM 677 CD LYS B 14 −3.830 22.53210.369 1.00 25.36 6 C ATOM 678 CE LYS B 14 −4.059 21.130 9.715 1.0027.62 6 C ATOM 679 NZ LYS B 14 −5.327 20.576 10.272 1.00 29.04 7 N ATOM680 C LYS B 14 −2.790 26.865 10.903 1.00 18.58 6 C ATOM 681 O LYS B 14−2.848 26.865 12.120 1.00 18.56 8 O ATOM 682 N GLU B 15 −2.503 27.91410.171 1.00 19.78 7 N ATOM 683 CA GLU B 15 −1.978 29.156 10.731 1.0021.26 6 C ATOM 684 CB GLU B 15 −1.998 30.269 9.681 1.00 22.71 6 C ATOM685 CG GLU B 15 −0.900 30.330 8.665 1.00 25.31 6 C ATOM 686 CD GLU B 15−1.205 29.466 7.441 1.00 28.76 6 C ATOM 687 OE1 GLU B 15 −2.177 28.6837.478 1.00 29.58 8 O ATOM 688 OE2 GLU B 15 −0.430 29.519 6.444 1.0029.72 8 O ATOM 689 C GLU B 15 −2.786 29.696 11.894 1.00 21.72 6 C ATOM690 O GLU B 15 −2.302 29.933 13.001 1.00 21.00 8 O ATOM 691 N ARG B 16−4.093 29.876 11.651 1.00 22.71 7 N ATOM 692 CA ARG B 16 −5.022 30.36612.661 1.00 22.83 6 C ATOM 693 CB ARG B 16 −6.422 30.552 12.073 1.0026.64 6 C ATOM 694 CG ARG B 16 −6.635 31.630 11.048 1.00 30.52 6 C ATOM695 CD ARG B 16 −6.555 33.048 11.602 1.00 35.34 6 C ATOM 696 NE ARG B 16−6.384 33.959 10.475 1.00 39.66 7 N ATOM 697 CZ ARG B 16 −6.162 35.25810.382 1.00 41.31 6 C ATOM 698 NH1 ARG B 16 −6.070 36.144 11.364 1.0042.11 7 N ATOM 699 NH2 ARG B 16 −6.050 35.658 9.104 1.00 41.95 7 N ATOM700 C ARG B 16 −5.125 29.436 13.851 1.00 21.36 6 C ATOM 701 O ARG B 16−5.111 29.904 15.001 1.00 21.13 8 O ATOM 702 N GLU B 17 −5.120 28.10713.675 1.00 19.62 7 N ATOM 703 CA GLU B 17 −5.244 27.313 14.924 1.0019.14 6 C ATOM 704 CB GLU B 17 −5.774 25.898 14.657 1.00 22.22 6 C ATOM705 CG GLU B 17 −4.832 25.235 13.662 1.00 26.79 6 C ATOM 706 CD GLU B 17−4.940 23.734 13.633 1.00 31.06 6 C ATOM 707 OE1 GLU B 17 −6.094 23.19813.654 1.00 33.97 8 O ATOM 708 OE2 GLU B 17 −3.794 23.223 13.550 1.0032.92 8 O ATOM 709 C GLU B 17 −3.922 27.312 15.672 1.00 16.82 6 C ATOM710 O GLU B 17 −3.902 27.254 16.914 1.00 16.12 8 O ATOM 711 N VAL B 18−2.801 27.360 14.949 1.00 16.92 7 N ATOM 712 CA VAL B 18 −1.508 27.47215.634 1.00 16.56 6 C ATOM 713 CB VAL B 18 −0.319 27.395 14.663 1.0016.85 6 C ATOM 714 CG1 VAL B 18 −1.050 27.672 15.311 1.00 16.29 6 C ATOM715 CG2 VAL B 18 −0.228 25.929 14.201 1.00 16.98 6 C ATOM 716 C VAL B 18−1.548 28.791 16.416 1.00 14.71 6 C ATOM 717 O VAL B 18 −1.156 28.84917.560 1.00 13.81 8 O ATOM 718 N MET B 19 −1.977 29.869 15.764 1.0015.18 7 N ATOM 719 CA MET B 19 −2.023 31.157 16.469 1.00 16.64 6 C ATOM720 CB MET B 19 −2.587 32.281 15.598 1.00 17.04 6 C ATOM 721 CG MET B 19−2.598 33.599 16.417 1.00 20.44 6 C ATOM 722 SD MET B 19 −2.923 34.98115.256 1.00 23.31 16 S ATOM 723 CE MET B 19 −1.290 34.646 14.586 1.0024.64 6 C ATOM 724 C MET B 19 −2.802 31.103 17.757 1.00 16.32 6 C ATOM725 O MET B 19 −2.336 31.571 18.803 1.00 13.62 8 O ATOM 726 N GLU B 20−3.983 30.445 17.736 1.00 18.19 7 N ATOM 727 CA GLU B 20 −4.773 30.39418.973 1.00 17.75 6 C ATOM 728 CB GLU B 20 −6.220 29.959 18.669 1.0020.39 6 C ATOM 729 CG GLU B 20 −6.902 30.808 17.607 1.00 22.11 6 C ATOM730 CD GLU B 20 −6.977 32.322 17.735 1.00 25.01 6 C ATOM 731 OE1 GLU B20 −7.101 32.971 18.786 1.00 22.59 8 O ATOM 732 OE2 GLU B 20 −6.86933.012 16.673 1.00 27.61 8 O ATOM 733 C GLU B 20 −4.070 29.571 20.0271.00 17.39 6 C ATOM 734 O GLU B 20 −4.083 30.001 21.202 1.00 15.65 8 OATOM 735 N LYS B 21 −3.400 28.447 19.654 1.00 15.60 7 N ATOM 736 CA LYSB 21 −2.647 27.767 20.701 1.00 14.17 6 C ATOM 737 CB LYS B 21 −1.96826.465 20.239 1.00 17.22 6 C ATOM 738 CG LYS B 21 −3.009 25.422 19.8061.00 20.24 6 C ATOM 739 CD LYS B 21 −2.479 24.019 19.721 1.00 20.81 6 CATOM 740 CE LYS B 21 −3.531 23.001 19.287 1.00 23.49 6 C ATOM 741 NZ LYSB 21 −3.546 22.690 17.821 1.00 24.31 7 N ATOM 742 C LYS B 21 −1.55928.704 21.255 1.00 12.69 6 C ATOM 743 O LYS B 21 −1.277 28.673 22.4501.00 9.62 8 O ATOM 744 N LEU B 22 −0.810 29.387 20.376 1.00 12.07 7 NATOM 745 CA LEU B 22 0.286 30.235 20.919 1.00 10.01 6 C ATOM 746 CB LEUB 22 0.994 30.891 19.717 1.00 10.07 6 C ATOM 747 CG LEU B 22 1.76529.929 18.772 1.00 11.87 6 C ATOM 748 CD1 LEU B 22 2.156 30.691 17.4841.00 10.37 6 C ATOM 749 CD2 LEU B 22 2.966 29.369 19.452 1.00 9.92 6 CATOM 750 C LEU B 22 −0.229 31.325 21.840 1.00 10.76 6 C ATOM 751 O LEU B22 0.328 31.647 22.890 1.00 11.14 8 O ATOM 752 N LEU B 23 −1.358 31.96021.525 1.00 9.40 7 N ATOM 753 CA LEU B 23 −1.921 33.024 22.345 1.0011.73 6 C ATOM 754 CB LEU B 23 −3.079 33.727 21.621 1.00 11.69 6 C ATOM755 CG LEU B 23 −2.745 34.512 20.356 1.00 11.81 6 C ATOM 756 CD1 LEU B23 −4.016 34.922 19.616 1.00 11.79 6 C ATOM 757 CD2 LEU B 23 −1.93835.790 20.695 1.00 12.26 6 C ATOM 758 C LEU B 23 −2.357 32.553 23.7271.00 13.43 6 C ATOM 759 O LEU B 23 −2.425 33.433 24.579 1.00 13.81 8 OATOM 760 N ALA B 24 −2.580 31.252 23.978 1.00 14.24 7 N ATOM 761 CA ALAB 24 −2.824 30.782 25.339 1.00 15.64 6 C ATOM 762 CB ALA B 24 −3.50229.408 25.345 1.00 15.75 6 C ATOM 763 C ALA B 24 −1.514 30.588 26.1041.00 16.64 6 C ATOM 764 O ALA B 24 −1.600 30.289 27.290 1.00 17.40 8 OATOM 765 N MET B 25 −0.349 30.681 25.462 1.00 14.70 7 N ATOM 766 CA METB 25 0.901 30.516 26.249 1.00 15.45 6 C ATOM 767 CB MET B 25 2.00129.980 25.331 1.00 14.21 6 C ATOM 768 CG MET B 25 1.578 28.734 24.5641.00 15.72 6 C ATOM 769 SD MET B 25 2.874 28.259 23.361 1.00 15.01 16 SATOM 770 CE MET B 25 2.028 26.880 22.566 1.00 14.16 6 C ATOM 771 C MET B25 1.267 31.832 26.875 1.00 13.99 6 C ATOM 772 O MET B 25 1.300 32.87226.197 1.00 13.82 8 O ATOM 773 N PRO B 26 1.573 31.882 28.180 1.00 15.207 N ATOM 774 CD PRO B 26 1.680 30.670 29.044 1.00 15.96 6 C ATOM 775 CAPRO B 26 1.948 33.094 28.857 1.00 14.72 6 C ATOM 776 CB PRO B 26 2.16032.681 30.320 1.00 15.60 6 C ATOM 777 CG PRO B 26 2.374 31.223 30.2791.00 15.14 6 C ATOM 778 C PRO B 26 3.255 33.744 28.338 1.00 14.07 6 CATOM 779 O PRO B 26 3.350 34.960 28.438 1.00 13.92 8 O ATOM 780 N GLU B27 4.170 33.002 27.747 1.00 11.08 7 N ATOM 781 CA GLU B 27 5.391 33.60327.183 1.00 13.20 6 C ATOM 782 CB GLU B 27 6.371 32.491 26.747 1.0013.28 6 C ATOM 783 CG GLU B 27 6.822 31.666 27.944 1.00 16.85 6 C ATOM784 CD GLU B 27 6.036 30.398 28.231 1.00 18.72 6 C ATOM 785 OE1 GLU B 274.890 30.149 27.819 1.00 18.84 8 O ATOM 786 OE2 GLU B 27 6.595 29.54028.940 1.00 19.28 8 O ATOM 787 C GLU B 27 5.057 34.427 25.939 1.00 11.336 C ATOM 788 O GLU B 27 5.821 35.259 25.512 1.00 11.54 8 O ATOM 789 NVAL B 28 3.990 34.102 25.231 1.00 10.97 7 N ATOM 790 CA VAL B 28 3.63434.767 23.972 1.00 10.37 6 C ATOM 791 CB VAL B 28 2.695 33.897 23.1211.00 10.11 6 C ATOM 792 CG1 VAL B 28 2.226 34.564 21.814 1.00 7.99 6 CATOM 793 CG2 VAL B 28 3.397 32.551 22.912 1.00 7.50 6 C ATOM 794 C VAL B28 2.948 36.083 24.254 1.00 11.91 6 C ATOM 795 O VAL B 28 1.843 36.13024.822 1.00 13.08 8 O ATOM 796 N LYS B 29 3.565 37.163 23.832 1.00 10.477 N ATOM 797 CA LYS B 29 3.011 38.482 24.072 1.00 10.88 6 C ATOM 798 CBLYS B 29 4.116 39.487 24.379 1.00 11.16 6 C ATOM 799 CG LYS B 29 5.04838.925 25.483 1.00 12.65 6 C ATOM 800 CD LYS B 29 4.298 38.989 26.8301.00 16.71 6 C ATOM 801 CE LYS B 29 4.738 37.821 27.706 1.00 19.48 6 CATOM 802 NZ LYS B 29 4.388 38.062 29.136 1.00 23.26 7 N ATOM 803 C LYS B29 2.264 38.986 22.859 1.00 11.85 6 C ATOM 804 O LYS B 29 1.369 39.85623.035 1.00 11.08 8 O ATOM 805 N GLU B 30 2.695 38.590 21.651 1.00 9.937 N ATOM 806 CA GLU B 30 1.998 39.086 20.459 1.00 9.60 6 C ATOM 807 CBGLU B 30 2.546 40.384 19.899 1.00 13.47 6 C ATOM 808 CG GLU B 30 2.57741.628 20.771 1.00 11.99 6 C ATOM 809 CD GLU B 30 3.193 42.838 20.1161.00 13.20 6 C ATOM 810 OE1 GLU B 30 3.579 43.769 20.856 1.00 11.60 8 OATOM 811 OE2 GLU B 30 3.107 42.989 18.886 1.00 13.89 8 O ATOM 812 C GLUB 30 2.104 37.967 19.437 1.00 11.24 6 C ATOM 813 O GLU B 30 3.137 37.25519.467 1.00 9.55 8 O ATOM 814 N ALA B 31 1.076 37.818 18.606 1.00 9.40 7N ATOM 815 CA ALA B 31 1.198 36.803 17.543 1.00 8.70 6 C ATOM 816 CB ALAB 31 0.698 35.429 17.973 1.00 7.99 6 C ATOM 817 C ALA B 31 0.357 37.31416.390 1.00 9.99 6 C ATOM 818 O ALA B 31 −0.761 37.841 16.609 1.00 10.678 O ATOM 819 N TYR B 32 0.854 37.192 15.160 1.00 7.33 7 N ATOM 820 CATYR B 32 0.119 37.639 14.013 1.00 7.98 6 C ATOM 821 CB TYR B 32 0.60438.961 13.443 1.00 6.68 6 C ATOM 822 CG TYR B 32 0.423 40.157 14.3121.00 8.26 6 C ATOM 823 CD1 TYR B 32 −0.728 40.934 14.064 1.00 8.39 6 CATOM 824 CE1 TYR B 32 −0.914 42.086 14.840 1.00 9.14 6 C ATOM 825 CD2TYR B 32 1.304 40.489 15.344 1.00 6.70 6 C ATOM 826 CE2 TYR B 32 1.08941.623 16.105 1.00 7.28 6 C ATOM 827 CZ TYR B 32 −0.020 42.387 15.8491.00 8.06 6 C ATOM 828 OH TYR B 32 −0.272 43.545 16.575 1.00 11.13 8 OATOM 829 C TYR B 32 0.359 36.711 12.791 1.00 9.26 6 C ATOM 830 O TYR B32 1.455 36.211 12.595 1.00 7.38 8 O ATOM 831 N VAL B 33 −0.774 36.44212.119 1.00 7.76 7 N ATOM 832 CA VAL B 33 −0.738 35.690 10.887 1.00 8.256 C ATOM 833 CB VAL B 33 −2.103 35.103 10.485 1.00 9.77 6 C ATOM 834 CG1VAL B 33 −1.928 34.416 9.141 1.00 9.20 6 C ATOM 835 CG2 VAL B 33 −2.58834.125 11.538 1.00 10.44 6 C ATOM 836 C VAL B 33 −0.313 36.695 9.8301.00 6.92 6 C ATOM 837 O VAL B 33 −0.847 37.804 9.767 1.00 8.47 8 O ATOM838 N VAL B 34 0.698 36.343 9.020 1.00 8.02 7 N ATOM 839 CA VAL B 341.176 37.342 8.101 1.00 8.86 6 C ATOM 840 CB VAL B 34 2.565 37.938 8.4401.00 8.81 6 C ATOM 841 CG1 VAL B 34 2.493 38.825 9.666 1.00 8.55 6 CATOM 842 CG2 VAL B 34 3.567 36.787 8.612 1.00 8.06 6 C ATOM 843 C VAL B34 1.265 36.792 6.688 1.00 11.20 6 C ATOM 844 O VAL B 34 1.427 35.6136.470 1.00 13.52 8 O ATOM 845 N TYR B 35 1.323 37.793 5.807 1.00 13.07 7N ATOM 846 CA TYR B 35 1.667 37.464 4.421 1.00 15.59 6 C ATOM 847 CB TYRB 35 0.814 38.417 3.594 1.00 17.52 6 C ATOM 848 CG TYR B 35 1.217 38.2092.156 1.00 21.88 6 C ATOM 849 CD1 TYR B 35 0.716 37.086 1.532 1.00 25.266 C ATOM 850 CE1 TYR B 35 1.050 36.814 0.212 1.00 28.20 6 C ATOM 851 CD2TYR B 35 2.102 39.034 1.521 1.00 24.78 6 C ATOM 852 CE2 TYR B 35 2.53038.731 0.240 1.00 26.74 6 C ATOM 853 CZ TYR B 35 1.938 37.685 −0.3981.00 28.56 6 C ATOM 854 OH TYR B 35 2.277 37.427 −1.709 1.00 33.78 8 OATOM 855 C TYR B 35 3.152 37.824 4.207 1.00 16.52 6 C ATOM 856 O TYR B35 3.517 38.932 4.657 1.00 18.14 8 O ATOM 857 N GLY B 36 3.904 37.0253.511 1.00 15.07 7 N ATOM 858 CA GLY B 36 5.297 37.441 3.214 1.00 18.366 C ATOM 859 C GLY B 36 6.143 36.151 3.231 1.00 19.05 6 C ATOM 860 O GLYB 36 5.557 35.061 3.058 1.00 16.65 8 O ATOM 861 N GLU B 37 7.434 36.2573.609 1.00 21.01 7 N ATOM 862 CA GLU B 37 8.152 34.953 3.595 1.00 23.516 C ATOM 863 CB GLU B 37 9.663 35.177 3.504 1.00 28.70 6 C ATOM 864 CGGLU B 37 10.269 35.983 4.630 1.00 32.35 6 C ATOM 865 CD GLU B 37 11.79835.903 4.612 1.00 36.24 6 C ATOM 866 OE1 GLU B 37 12.437 36.685 5.3721.00 35.75 8 O ATOM 867 OE2 GLU B 37 12.317 35.044 3.847 1.00 37.65 8 OATOM 868 C GLU B 37 7.845 33.981 4.714 1.00 23.07 6 C ATOM 869 O GLU B37 8.259 32.834 4.540 1.00 23.02 8 O ATOM 870 N TYR B 38 7.232 34.3375.840 1.00 21.40 7 N ATOM 871 CA TYR B 38 6.879 33.361 6.889 1.00 19.676 C ATOM 872 CB TYR B 38 7.610 33.823 8.162 1.00 20.72 6 C ATOM 873 CGTYR B 38 9.064 33.414 8.146 1.00 23.64 6 C ATOM 874 CD1 TYR B 38 9.40132.089 8.412 1.00 25.30 6 C ATOM 875 CE1 TYR B 38 10.714 31.666 8.4161.00 26.52 6 C ATOM 876 CD2 TYR B 38 10.090 34.313 7.871 1.00 25.13 6 CATOM 877 CE2 TYR B 38 11.410 33.895 7.884 1.00 25.61 6 C ATOM 878 CZ TYRB 38 11.710 32.591 8.145 1.00 27.23 6 C ATOM 879 OH TYR B 38 13.03532.149 8.152 1.00 29.72 8 O ATOM 880 C TYR B 38 5.376 33.329 7.149 1.0017.30 6 C ATOM 881 O TYR B 38 4.744 34.238 6.603 1.00 16.96 8 O ATOM 882N ASP B 39 4.742 32.399 7.856 1.00 16.10 7 N ATOM 883 CA ASP B 39 3.31832.359 8.062 1.00 15.22 6 C ATOM 884 CB ASP B 39 2.879 30.893 8.284 1.0017.21 6 C ATOM 885 CG ASP B 39 2.939 30.170 6.935 1.00 19.01 6 C ATOM886 OD1 ASP B 39 2.735 30.787 5.869 1.00 19.17 8 O ATOM 887 OD2 ASP B 393.263 28.976 7.028 1.00 20.31 8 O ATOM 888 C ASP B 39 2.815 33.108 9.2971.00 15.48 6 C ATOM 889 O ASP B 39 1.726 33.694 9.290 1.00 13.65 8 OATOM 890 N LEU B 40 3.590 33.064 10.383 1.00 13.35 7 N ATOM 891 CA LEU B40 3.266 33.717 11.639 1.00 12.10 6 C ATOM 892 CB LEU B 40 2.872 32.70812.724 1.00 13.67 6 C ATOM 893 CG LEU B 40 1.601 31.893 12.552 1.0017.69 6 C ATOM 894 CD1 LEU B 40 1.329 30.939 13.715 1.00 20.32 6 C ATOM895 CD2 LEU B 40 0.394 32.825 12.479 1.00 20.29 6 C ATOM 896 C LEU B 404.471 34.470 12.223 1.00 11.17 6 C ATOM 897 O LEU B 40 5.646 34.11811.961 1.00 10.75 8 O ATOM 898 N ILE B 41 4.237 35.543 12.943 1.00 8.247 N ATOM 899 CA ILE B 41 5.339 36.230 13.626 1.00 9.66 6 C ATOM 900 CBILE B 41 5.712 37.587 13.108 1.00 12.11 6 C ATOM 901 CG2 ILE B 41 4.55738.563 13.016 1.00 15.03 6 C ATOM 902 CG1 ILE B 41 6.735 38.248 14.0961.00 13.84 6 C ATOM 903 CD1 ILE B 41 7.691 39.105 13.319 1.00 15.84 6 CATOM 904 C ILE B 41 4.851 36.269 15.074 1.00 10.80 6 C ATOM 905 O ILE B41 3.678 36.570 15.329 1.00 9.87 8 O ATOM 906 N VAL B 42 5.666 35.85316.019 1.00 9.90 7 N ATOM 907 CA VAL B 42 5.269 35.779 17.415 1.00 8.616 C ATOM 908 CB VAL B 42 5.231 34.269 17.758 1.00 11.63 6 C ATOM 909 CG1VAL B 42 4.728 33.994 19.179 1.00 11.77 6 C ATOM 910 CG2 VAL B 42 4.38933.446 16.776 1.00 11.85 6 C ATOM 911 C VAL B 42 6.265 36.480 18.3071.00 9.85 6 C ATOM 912 O VAL B 42 7.476 36.173 18.143 1.00 9.17 8 O ATOM913 N LYS B 43 5.811 37.419 19.136 1.00 7.78 7 N ATOM 914 CA LYS B 436.740 38.088 20.055 1.00 7.65 6 C ATOM 915 CB LYS B 43 6.282 39.51920.345 1.00 9.86 6 C ATOM 916 CG LYS B 43 7.306 40.251 21.245 1.00 10.716 C ATOM 917 CD LYS B 43 6.614 41.604 21.531 1.00 9.74 6 C ATOM 918 CELYS B 43 7.585 42.476 22.315 1.00 10.47 6 C ATOM 919 NZ LYS B 43 6.92043.783 22.618 1.00 6.81 7 N ATOM 920 C LYS B 43 6.694 37.349 21.396 1.009.77 6 C ATOM 921 O LYS B 43 5.585 37.200 21.979 1.00 8.09 8 O ATOM 922N VAL B 44 7.840 36.891 21.897 1.00 8.63 7 N ATOM 923 CA VAL B 44 7.81836.118 23.143 1.00 8.80 6 C ATOM 924 CB VAL B 44 8.104 34.612 22.9731.00 10.38 6 C ATOM 925 CG1 VAL B 44 7.208 33.936 21.922 1.00 8.21 6 CATOM 926 CG2 VAL B 44 9.563 34.281 22.597 1.00 9.95 6 C ATOM 927 C VAL B44 8.782 36.742 24.138 1.00 9.71 6 C ATOM 928 O VAL B 44 9.699 37.51023.750 1.00 5.89 8 O ATOM 929 N GLU B 45 8.479 36.528 25.418 1.00 10.097 N ATOM 930 CA GLU B 45 9.296 37.043 26.518 1.00 13.68 6 C ATOM 931 CBGLU B 45 8.689 38.315 27.137 1.00 14.51 6 C ATOM 932 CG GLU B 45 8.79839.552 26.269 1.00 18.31 6 C ATOM 933 CD GLU B 45 8.037 40.752 26.7921.00 21.50 6 C ATOM 934 OE1 GLU B 45 7.964 41.733 26.010 1.00 22.57 8 OATOM 935 OE2 GLU B 45 7.590 40.680 27.967 1.00 20.85 8 O ATOM 936 C GLUB 45 9.424 35.985 27.622 1.00 15.23 6 C ATOM 937 O GLU B 45 8.491 35.24327.941 1.00 12.79 8 O ATOM 938 N THR B 46 10.617 35.776 28.144 1.0015.49 7 N ATOM 939 CA THR B 46 10.988 34.815 29.157 1.00 15.12 6 C ATOM940 CB THR B 46 11.682 33.534 28.689 1.00 16.31 6 C ATOM 941 OG1 THR B46 12.959 33.763 28.010 1.00 15.78 8 O ATOM 942 CC2 THR B 46 10.83332.730 27.693 1.00 17.84 6 C ATOM 943 C THR B 46 11.930 35.522 30.1421.00 17.32 6 C ATOM 944 O THR B 46 12.524 36.584 29.858 1.00 16.70 8 OATOM 945 N ASP B 47 12.010 34.957 31.363 1.00 17.97 7 N ATOM 946 CA ASPB 47 12.845 35.635 32.353 1.00 20.36 6 C ATOM 947 CB ASP B 47 12.59835.223 33.813 1.00 25.58 6 C ATOM 948 CG ASP B 47 12.664 33.736 34.0351.00 29.81 6 C ATOM 949 OD1 ASP B 47 12.448 33.010 33.027 1.00 31.62 8 OATOM 950 OD2 ASP B 47 12.956 33.250 35.162 1.00 32.24 8 O ATOM 951 C ASPB 47 14.301 35.373 31.978 1.00 16.72 6 C ATOM 952 O ASP B 47 15.11236.307 32.039 1.00 17.65 8 O ATOM 953 N THR B 48 14.620 34.173 31.5511.00 15.74 7 N ATOM 954 CA THR B 48 16.043 33.987 31.213 1.00 16.40 6 CATOM 955 CB THR B 48 16.770 33.069 32.215 1.00 15.67 6 C ATOM 956 OG1THR B 48 16.288 31.739 31.991 1.00 16.49 8 O ATOM 957 CG2 THR B 4816.605 33.444 33.691 1.00 17.54 6 C ATOM 958 C THR B 48 16.231 33.40529.817 1.00 15.74 6 C ATOM 959 O THR B 48 15.286 32.952 29.210 1.0016.51 8 O ATOM 960 N LEU B 49 17.488 33.424 29.331 1.00 14.22 7 N ATOM961 CA LEU B 49 17.828 32.808 28.078 1.00 12.99 6 C ATOM 962 CB LEU B 4919.309 33.047 27.731 1.00 13.45 6 C ATOM 963 CG LEU B 49 19.772 32.44226.399 1.00 12.86 6 C ATOM 964 CD1 LEU B 49 18.885 32.972 25.269 1.0012.52 6 C ATOM 965 CD2 LEU B 49 21.248 32.799 26.176 1.00 12.69 6 C ATOM966 C LEU B 49 17.641 31.314 28.138 1.00 14.59 6 C ATOM 967 O LEU B 4917.102 30.681 27.223 1.00 15.53 8 O ATOM 968 N LYS B 50 17.970 30.68229.262 1.00 16.17 7 N ATOM 969 CA LYS B 50 17.720 29.246 29.381 1.0019.48 6 C ATOM 970 CB LYS B 50 18.193 28.727 30.758 1.00 22.06 6 C ATOM971 CG LYS B 50 19.650 28.768 31.070 1.00 27.90 6 C ATOM 972 CD LYS B 5020.542 27.872 30.226 1.00 30.91 6 C ATOM 973 CE LYS B 50 21.605 27.20531.107 1.00 34.16 6 C ATOM 974 NZ LYS B 50 22.915 27.924 31.080 1.0035.27 7 N ATOM 975 C LYS B 50 16.214 28.924 29.306 1.00 14.90 6 C ATOM976 O LYS B 50 15.819 27.823 28.917 1.00 14.68 8 O ATOM 977 N ASP B 5115.380 29.793 29.876 1.00 17.81 7 N ATOM 978 CA ASP B 51 13.908 29.50729.814 1.00 17.69 6 C ATOM 979 CB ASP B 51 13.219 30.482 30.729 1.0019.51 6 C ATOM 980 CG ASP B 51 13.360 30.134 32.206 1.00 20.72 6 C ATOM981 OD1 ASP B 51 13.708 29.003 32.574 1.00 21.31 8 O ATOM 982 OD2 ASP B51 13.092 31.053 32.984 1.00 20.55 8 O ATOM 983 C ASP B 51 13.371 29.63228.398 1.00 16.44 6 C ATOM 984 O ASP B 51 12.715 28.703 27.890 1.0017.42 8 O ATOM 985 N LEU B 52 13.941 30.582 27.633 1.00 15.23 7 N ATOM986 CA LEU B 52 13.645 30.699 26.207 1.00 13.82 6 C ATOM 987 CB LEU B 5214.396 31.920 25.587 1.00 14.63 6 C ATOM 988 CG LEU B 52 14.076 32.19424.117 1.00 15.53 6 C ATOM 989 CD1 LEU B 52 12.710 32.945 24.006 1.0014.67 6 C ATOM 990 CD2 LEU B 52 15.158 32.974 23.399 1.00 13.85 6 C ATOM991 C LEU B 52 14.079 29.449 25.476 1.00 14.79 6 C ATOM 992 O LEU B 5213.369 28.870 24.658 1.00 14.97 8 O ATOM 993 N ASP B 53 15.306 28.96725.785 1.00 14.38 7 N ATOM 994 CA ASP B 53 15.821 27.759 25.148 1.0016.61 6 C ATOM 995 CB ASP B 53 17.158 27.294 25.758 1.00 16.33 6 C ATOM996 CG ASP B 53 18.329 28.180 25.461 1.00 17.82 6 C ATOM 997 OD1 ASP B53 18.339 29.043 24.561 1.00 16.77 8 O ATOM 998 OD2 ASP B 53 19.34028.168 26.224 1.00 21.96 8 O ATOM 999 C ASP B 53 14.800 26.626 25.2311.00 16.88 6 C ATOM 1000 O ASP B 53 14.575 25.898 24.270 1.00 16.79 8 OATOM 1001 N GLN B 54 14.351 26.352 26.437 1.00 19.65 7 N ATOM 1002 CAGLN B 54 13.406 25.269 26.733 1.00 23.28 6 C ATOM 1003 CB GLN B 5413.240 25.185 28.249 1.00 26.46 6 C ATOM 1004 CG GLN B 54 12.272 24.20028.879 1.00 32.94 6 C ATOM 1005 CD GLN B 54 12.330 24.433 30.398 1.0037.85 6 C ATOM 1006 OE1 GLN B 54 11.846 25.474 30.887 1.00 39.47 8 OATOM 1007 NE2 GLN B 54 12.945 23.515 31.160 1.00 38.13 7 N ATOM 1008 CGLN B 54 12.051 25.545 26.083 1.00 21.13 6 C ATOM 1009 O GLN B 54 11.49924.633 25.489 1.00 23.10 8 O ATOM 1010 N PHE B 55 11.558 26.769 26.1351.00 20.48 7 N ATOM 1011 CA PHE B 55 10.290 27.164 25.514 1.00 17.72 6 CATOM 1012 CB PHE B 55 9.979 28.639 25.834 1.00 17.24 6 C ATOM 1013 CGPHE B 55 8.715 29.086 25.132 1.00 17.59 6 C ATOM 1014 CD1 PHE B 55 7.47928.564 25.536 1.00 16.74 6 C ATOM 1015 CD2 PHE B 55 8.753 29.980 24.0751.00 16.08 6 C ATOM 1016 CE1 PHE B 55 6.317 28.943 24.873 1.00 15.53 6 CATOM 1017 CE2 PHE B 55 7.594 30.392 23.472 1.00 15.86 6 C ATOM 1018 CZPHE B 55 6.369 29.852 23.853 1.00 16.04 6 C ATOM 1019 C PHE B 55 10.27126.871 24.028 1.00 17.99 6 C ATOM 1020 O PHE B 55 9.434 26.131 23.4711.00 14.83 8 O ATOM 1021 N ILE B 56 11.365 27.316 23.361 1.00 16.17 7 NATOM 1022 CA ILE B 56 11.462 27.092 21.932 1.00 17.08 6 C ATOM 1023 CBILE B 56 12.504 28.050 21.294 1.00 17.13 6 C ATOM 1024 CG2 ILE B 5612.639 27.710 19.833 1.00 17.70 6 C ATOM 1025 CG1 ILE B 56 11.991 29.47021.490 1.00 18.12 6 C ATOM 1026 CD1 ILE B 56 12.819 30.561 20.895 1.0022.50 6 C ATOM 1027 C ILE B 56 11.776 25.645 21.585 1.00 17.95 6 C ATOM1028 O ILE B 56 11.122 25.106 20.683 1.00 17.47 8 O ATOM 1029 N THR B 5712.732 24.990 22.239 1.00 18.22 7 N ATOM 1030 CA THR B 57 13.053 23.63221.790 1.00 20.69 6 C ATOM 1031 CB THR B 57 14.411 23.126 22.315 1.0020.99 6 C ATOM 1032 OG1 THR B 57 14.470 23.336 23.722 1.00 23.13 8 OATOM 1033 CG2 THR B 57 15.565 23.877 21.657 1.00 21.52 6 C ATOM 1034 CTHR B 57 11.986 22.615 22.194 1.00 22.22 6 C ATOM 1035 O THR B 57 11.71221.730 21.385 1.00 21.32 8 O ATOM 1036 N GLU B 58 11.456 22.829 23.3921.00 23.89 7 N ATOM 1037 CA GLU B 58 10.513 21.872 23.941 1.00 28.94 6 CATOM 1038 CB GLU B 58 10.777 21.648 25.449 1.00 30.54 6 C ATOM 1039 CGGLU B 58 12.155 21.058 25.706 1.00 33.21 6 C ATOM 1040 CD GLU B 5812.442 19.835 24.844 1.00 36.23 6 C ATOM 1041 OE1 GLU B 58 11.537 18.98924.608 1.00 36.52 8 O ATOM 1042 OE2 GLU B 58 13.596 19.742 24.361 1.0036.20 8 O ATOM 1043 C GLU B 58 9.052 22.194 23.689 1.00 27.95 6 C ATOM1044 O GLU B 58 8.347 21.187 23.496 1.00 30.27 8 O ATOM 1045 N LYS B 598.594 23.432 23.616 1.00 26.94 7 N ATOM 1046 CA LYS B 59 7.178 23.64823.327 1.00 25.11 6 C ATOM 1047 CB LYS B 59 6.578 24.692 24.255 1.0027.02 6 C ATOM 1048 CG LYS B 59 6.244 24.161 25.639 1.00 30.90 6 C ATOM1049 CD LYS B 59 5.624 25.236 26.549 1.00 34.58 6 C ATOM 1050 CE LYS B59 5.699 24.798 28.020 1.00 34.94 6 C ATOM 1051 NZ LYS B 59 5.725 25.94428.972 1.00 36.11 7 N ATOM 1052 C LYS B 59 6.911 24.004 21.857 1.0023.87 6 C ATOM 1053 O LYS B 59 6.423 23.147 21.109 1.00 23.36 8 O ATOM1054 N ILE B 60 7.548 25.056 21.351 1.00 19.85 7 N ATOM 1055 CA ILE B 607.274 25.554 20.003 1.00 17.05 6 C ATOM 1056 CB ILE B 60 8.007 26.90519.765 1.00 15.53 6 C ATOM 1057 CG2 ILE B 60 7.825 27.435 18.363 1.0013.69 6 C ATOM 1058 CG1 ILE B 60 7.481 27.986 20.736 1.00 16.51 6 C ATOM1059 CD1 ILE B 60 5.991 28.251 20.476 1.00 15.98 6 C ATOM 1060 C ILE B60 7.609 24.563 18.917 1.00 17.34 6 C ATOM 1061 O ILE B 60 6.772 24.16218.130 1.00 14.52 8 O ATOM 1062 N ARG B 61 8.875 24.103 18.890 1.0018.70 7 N ATOM 1063 CA ARG B 61 9.371 23.212 17.852 1.00 20.52 6 C ATOM1064 CB ARG B 61 10.920 23.301 17.802 1.00 20.89 6 C ATOM 1065 CG ARG B61 11.306 24.662 17.216 1.00 21.37 6 C ATOM 1066 CD ARG B 61 12.78824.911 17.048 1.00 21.93 6 C ATOM 1067 NE ARG B 61 13.411 24.028 16.0801.00 21.23 7 N ATOM 1068 CZ ARG B 61 13.511 24.024 14.776 1.00 18.33 6 CATOM 1069 NH1 ARG B 61 12.968 24.992 14.081 1.00 18.18 7 N ATOM 1070 NH2ARG B 61 14.210 23.051 14.197 1.00 22.37 7 N ATOM 1071 C ARG B 61 8.84921.789 17.877 1.00 20.48 6 C ATOM 1072 O ARG B 61 8.872 21.139 16.8231.00 19.46 8 O ATOM 1073 N LYS B 62 8.189 21.397 18.948 1.00 19.89 7 NATOM 1074 CA LYS B 62 7.486 20.126 19.010 1.00 22.12 6 C ATOM 1075 CBLYS B 62 7.564 19.510 20.415 1.00 22.87 6 C ATOM 1076 CG LYS B 62 8.99819.480 20.908 1.00 25.05 6 C ATOM 1077 CD LYS B 62 9.842 18.467 20.1321.00 26.73 6 C ATOM 1078 CE LYS B 62 11.164 18.267 20.879 1.00 28.67 6 CATOM 1079 NZ LYS B 62 11.865 17.052 20.376 1.00 29.88 7 N ATOM 1080 CLYS B 62 6.018 20.266 18.653 1.00 22.89 6 C ATOM 1081 O LYS B 62 5.37119.221 18.738 1.00 24.68 8 O ATOM 1082 N MET B 63 5.493 21.455 18.3461.00 22.05 7 N ATOM 1083 CA MET B 63 4.085 21.535 17.935 1.00 21.24 6 CATOM 1084 CB MET B 63 3.596 22.986 17.770 1.00 21.67 6 C ATOM 1085 CGMET B 63 3.515 23.697 19.108 1.00 24.24 6 C ATOM 1086 SD MET B 63 2.95825.403 19.105 1.00 25.86 16 S ATOM 1087 CE MET B 63 1.266 25.330 18.5461.00 22.54 6 C ATOM 1088 C MET B 63 3.994 20.877 16.578 1.00 20.21 6 CATOM 1089 O MET B 63 4.679 21.365 15.693 1.00 19.21 8 O ATOM 1090 N PROB 64 3.013 20.020 16.328 1.00 20.66 7 N ATOM 1091 CD PRO B 64 2.17119.423 17.400 1.00 21.16 6 C ATOM 1092 CA PRO B 64 3.009 19.225 15.1131.00 20.71 6 C ATOM 1093 CB PRO B 64 1.914 18.185 15.357 1.00 22.37 6 CATOM 1094 CG PRO B 64 1.645 18.137 16.824 1.00 20.69 6 C ATOM 1095 C PROB 64 2.817 20.011 13.840 1.00 20.17 6 C ATOM 1096 O PRO B 64 3.34419.668 12.765 1.00 19.17 8 O ATOM 1097 N GLU B 65 2.077 21.113 13.9281.00 18.92 7 N ATOM 1098 CA GLU B 65 1.808 21.925 12.750 1.00 20.82 6 CATOM 1099 CB GLU B 65 0.451 22.625 12.962 1.00 23.06 6 C ATOM 1100 CGGLU B 65 −0.570 21.732 13.649 1.00 24.65 6 C ATOM 1101 CD GLU B 65−0.701 22.037 15.135 1.00 28.89 6 C ATOM 1102 OE1 GLU B 65 0.281 21.99915.947 1.00 26.57 8 O ATOM 1103 OE2 GLU B 65 −1.900 22.338 15.495 1.0030.49 8 O ATOM 1104 C GLU B 65 2.866 22.965 12.446 1.00 20.76 6 C ATOM1105 O GLU B 65 2.822 23.524 11.345 1.00 23.34 8 O ATOM 1106 N ILE B 663.880 23.130 13.292 1.00 19.85 7 N ATOM 1107 CA ILE B 66 4.988 24.06612.991 1.00 18.23 6 C ATOM 1108 CB ILE B 66 5.574 24.580 14.324 1.0018.27 6 C ATOM 1109 CG2 ILE B 66 6.951 25.202 14.145 1.00 18.20 6 C ATOM1110 CG1 ILE B 66 4.502 25.528 14.888 1.00 18.67 6 C ATOM 1111 CD1 ILE B66 4.911 26.360 16.092 1.00 19.78 6 C ATOM 1112 C ILE B 66 6.023 23.33112.155 1.00 19.56 6 C ATOM 1113 O ILE B 66 6.493 22.275 12.616 1.0018.62 8 O ATOM 1114 N GLN B 67 6.373 23.821 10.957 1.00 18.63 7 N ATOM1115 CA GLN B 67 7.296 23.106 10.117 1.00 19.98 6 C ATOM 1116 CB GLN B67 6.901 23.271 8.625 1.00 21.88 6 C ATOM 1117 CG GLN B 67 5.496 22.7628.421 1.00 26.02 6 C ATOM 1118 CD GLN B 67 5.042 22.459 7.026 1.00 28.556 C ATOM 1119 OE1 GLN B 67 4.361 21.434 6.858 1.00 31.69 8 O ATOM 1120NE2 GLN B 67 5.284 23.306 6.034 1.00 29.80 7 N ATOM 1121 C GLN B 678.706 23.681 10.243 1.00 21.32 6 C ATOM 1122 O GLN B 67 9.715 23.0999.843 1.00 20.33 8 O ATOM 1123 N MET B 68 8.749 24.966 10.583 1.00 21.167 N ATOM 1124 CA MET B 68 10.009 25.693 10.650 1.00 20.94 6 C ATOM 1125CB MET B 68 10.295 26.285 9.262 1.00 24.01 6 C ATOM 1126 CG MET B 6810.902 25.394 8.237 1.00 30.45 6 C ATOM 1127 SD MET B 68 11.853 26.2817.020 1.00 38.87 16 S ATOM 1128 CE MET B 68 10.602 26.712 5.822 1.0035.81 6 C ATOM 1129 C MET B 68 9.911 26.899 11.558 1.00 17.78 6 C ATOM1130 O MET B 68 8.870 27.576 11.556 1.00 16.20 8 O ATOM 1131 N THR B 6910.977 27.268 12.235 1.00 15.58 7 N ATOM 1132 CA THR B 69 11.017 28.50613.025 1.00 12.38 6 C ATOM 1133 CB THR B 69 10.793 28.278 14.527 1.0013.97 6 C ATOM 1134 OG1 THR B 69 11.853 27.497 15.116 1.00 14.07 8 OATOM 1135 CG2 THR B 69 9.458 27.596 14.822 1.00 11.50 6 C ATOM 1136 CTHR B 69 12.360 29.171 12.790 1.00 14.55 6 C ATOM 1137 O THR B 69 13.36428.457 12.587 1.00 13.00 8 O ATOM 1138 N SER B 70 12.431 30.484 12.9261.00 11.76 7 N ATOM 1139 CA SER B 70 13.653 31.254 12.816 1.00 11.23 6 CATOM 1140 CB SER B 70 13.770 31.802 11.403 1.00 10.94 6 C ATOM 1141 OGSER B 70 14.807 32.763 11.395 1.00 13.40 8 O ATOM 1142 C SER B 70 13.46732.327 13.909 1.00 13.08 6 C ATOM 1143 O SER B 70 12.493 33.086 13.7941.00 11.11 8 O ATOM 1144 N THR B 71 14.234 32.225 15.001 1.00 9.49 7 NATOM 1145 CA THR B 71 14.004 33.081 16.152 1.00 9.27 6 C ATOM 1146 CBTHR B 71 14.023 32.166 17.406 1.00 11.05 6 C ATOM 1147 OG1 THR B 7112.964 31.212 17.347 1.00 10.77 8 O ATOM 1148 CG2 THR B 71 13.880 33.01818.664 1.00 12.37 6 C ATOM 1149 C THR B 71 15.047 34.188 16.282 1.008.22 6 C ATOM 1150 O THR B 71 16.249 33.931 16.119 1.00 10.11 8 O ATOM1151 N MET B 72 14.635 35.405 16.449 1.00 6.69 7 N ATOM 1152 CA MET B 7215.517 36.547 16.585 1.00 7.80 6 C ATOM 1153 CB MET B 72 15.111 37.66215.627 1.00 10.55 6 C ATOM 1154 CG MET B 72 15.251 37.183 14.165 1.0016.67 6 C ATOM 1155 SD MET B 72 14.478 38.386 13.048 1.00 20.04 16 SATOM 1156 CE MET B 72 14.819 37.455 11.545 1.00 23.19 6 C ATOM 1157 CMET B 72 15.467 37.079 18.005 1.00 7.77 6 C ATOM 1158 O MET B 72 14.44237.688 18.349 1.00 7.18 8 O ATOM 1159 N ILE B 73 16.559 36.867 18.7441.00 6.41 7 N ATOM 1160 CA ILE B 73 16.580 37.347 20.117 1.00 8.84 6 CATOM 1161 CB ILE B 73 17.656 36.575 20.926 1.00 7.95 6 C ATOM 1162 CG2ILE B 73 17.878 37.273 22.301 1.00 9.41 6 C ATOM 1163 CG1 ILE B 7317.217 35.102 21.017 1.00 8.05 6 C ATOM 1164 CD1 ILE B 73 18.284 34.10321.467 1.00 11.03 6 C ATOM 1165 C ILE B 73 16.774 38.842 20.097 1.0011.08 6 C ATOM 1166 O ILE B 73 17.663 39.307 19.357 1.00 12.77 8 O ATOM1167 N ALA B 74 16.112 39.633 20.955 1.00 11.74 7 N ATOM 1168 CA ALA B74 16.349 41.049 21.012 1.00 11.91 6 C ATOM 1169 CB ALA B 74 15.19041.968 21.481 1.00 11.66 6 C ATOM 1170 C ALA B 74 17.560 41.357 21.9041.00 14.51 6 C ATOM 1171 O ALA B 74 17.794 40.714 22.942 1.00 11.20 8 OATOM 1172 N ILE B 75 18.109 42.519 21.630 1.00 17.53 7 N ATOM 1173 CAILE B 75 19.305 43.071 22.262 1.00 24.22 6 C ATOM 1174 CB ILE B 7520.071 43.938 21.234 1.00 25.52 6 C ATOM 1175 CG2 ILE B 75 20.752 45.18121.763 1.00 27.56 6 C ATOM 1176 CG1 ILE B 75 21.106 43.078 20.489 1.0025.82 6 C ATOM 1177 CD1 ILE B 75 22.210 42.628 21.402 1.00 28.79 6 CATOM 1178 C ILE B 75 18.913 43.902 23.470 1.00 27.45 6 C ATOM 1179 O ILEB 75 17.779 44.405 23.469 1.00 29.37 8 O ATOM 1180 OT ILE B 75 19.76244.028 24.395 1.00 31.78 8 O ATOM 1181 CB VAL C 2 39.332 19.443 4.1711.00 13.51 6 C ATOM 1182 CG1 VAL C 2 38.145 20.355 3.825 1.00 13.69 6 CATOM 1183 CG2 VAL C 2 40.295 19.357 2.968 1.00 11.61 6 C ATOM 1184 C VALC 2 39.163 20.233 6.562 1.00 13.74 6 C ATOM 1185 O VAL C 2 38.668 19.2807.202 1.00 13.05 8 O ATOM 1186 N VAL C 2 41.199 18.998 5.711 1.00 13.507 N ATOM 1187 CA VAL C 2 40.102 19.970 5.380 1.00 13.12 6 C ATOM 1188 NTHR C 3 39.031 21.486 6.942 1.00 13.22 7 N ATOM 1189 CA THR C 3 38.16821.900 8.039 1.00 14.11 6 C ATOM 1190 CB THR C 3 38.833 22.798 9.0841.00 12.14 6 C ATOM 1191 OG1 THR C 3 39.931 22.193 9.744 1.00 12.53 8 OATOM 1192 CG2 THR C 3 37.829 23.094 10.246 1.00 10.76 6 C ATOM 1193 CTHR C 3 36.960 22.646 7.426 1.00 13.61 6 C ATOM 1194 O THR C 3 37.20223.489 6.563 1.00 12.66 8 O ATOM 1195 N ALA C 4 35.746 22.325 7.836 1.0011.41 7 N ATOM 1196 CA ALA C 4 34.586 22.976 7.285 1.00 11.20 6 C ATOM1197 CB ALA C 4 33.979 22.223 6.107 1.00 8.89 6 C ATOM 1198 C ALA C 433.477 23.136 8.345 1.00 11.12 6 C ATOM 1199 O ALA C 4 33.347 22.3229.266 1.00 10.90 8 O ATOM 1200 N PHE C 5 32.693 24.214 8.185 1.00 9.67 7N ATOM 1201 CA PHE C 5 31.550 24.453 9.036 1.00 9.21 6 C ATOM 1202 CBPHE C 5 31.422 25.860 9.633 1.00 9.50 6 C ATOM 1203 CG PHE C 5 32.39726.100 10.752 1.00 10.21 6 C ATOM 1204 CD1 PHE C 5 33.702 26.499 10.4281.00 8.38 6 C ATOM 1205 CD2 PHE C 5 32.076 25.900 12.080 1.00 9.76 6 CATOM 1206 CE1 PHE C 5 34.665 26.663 11.392 1.00 10.44 6 C ATOM 1207 CE2PHE C 5 33.051 26.086 13.050 1.00 8.18 6 C ATOM 1208 CZ PHE C 5 34.31826.446 12.727 1.00 9.99 6 C ATOM 1209 C PHE C 5 30.312 24.105 8.196 1.0011.44 6 C ATOM 1210 O PHE C 5 30.128 24.588 7.060 1.00 13.44 8 O ATOM1211 N ILE C 6 29.458 23.243 8.739 1.00 9.23 7 N ATOM 1212 CA ILE C 628.227 22.977 7.980 1.00 10.67 6 C ATOM 1213 CB ILE C 6 28.075 21.4537.793 1.00 12.19 6 C ATOM 1214 CG2 ILE C 6 26.744 21.189 7.118 1.0011.24 6 C ATOM 1215 CG1 ILE C 6 29.268 20.837 7.020 1.00 12.66 6 C ATOM1216 CD1 ILE C 6 29.037 19.318 6.949 1.00 12.74 6 C ATOM 1217 C ILE C 627.055 23.571 8.739 1.00 9.95 6 C ATOM 1218 O ILE C 6 26.932 23.2459.922 1.00 7.94 8 O ATOM 1219 N LEU C 7 26.275 24.443 8.129 1.00 10.46 7N ATOM 1220 CA LEU C 7 25.123 25.054 8.768 1.00 11.52 6 C ATOM 1221 CBLEU C 7 24.963 26.522 8.306 1.00 14.75 6 C ATOM 1222 CG LEU C 7 26.16327.363 8.764 1.00 16.86 6 C ATOM 1223 CD1 LEU C 7 25.971 28.807 8.3101.00 19.73 6 C ATOM 1224 CD2 LEU C 7 26.301 27.276 10.262 1.00 17.56 6 CATOM 1225 C LEU C 7 23.887 24.324 8.262 1.00 13.74 6 C ATOM 1226 O LEU C7 23.864 24.231 7.024 1.00 15.75 8 O ATOM 1227 N MET C 8 22.967 23.8619.073 1.00 12.28 7 N ATOM 1228 CA MET C 8 21.871 23.050 8.619 1.00 15.306 C ATOM 1229 CB MET C 8 22.064 21.613 9.116 1.00 16.92 6 C ATOM 1230 CGMET C 8 23.275 20.809 8.666 1.00 18.76 6 C ATOM 1231 SD MET C 8 23.60019.314 9.582 1.00 20.83 16 S ATOM 1232 CE MET C 8 24.791 19.921 10.7761.00 24.28 6 C ATOM 1233 C MET C 8 20.510 23.492 9.187 1.00 17.65 6 CATOM 1234 O MET C 8 20.409 23.926 10.346 1.00 16.91 8 O ATOM 1235 N VALC 9 19.496 23.240 8.362 1.00 19.14 7 N ATOM 1236 CA VAL C 9 18.08323.374 8.761 1.00 19.28 6 C ATOM 1237 CB VAL C 9 17.168 23.967 7.7181.00 19.18 6 C ATOM 1238 CG1 VAL C 9 15.697 23.905 8.170 1.00 19.22 6 CATOM 1239 CG2 VAL C 9 17.579 25.418 7.540 1.00 19.16 6 C ATOM 1240 C VALC 9 17.598 21.966 9.060 1.00 19.88 6 C ATOM 1241 O VAL C 9 18.106 21.0358.414 1.00 23.28 8 O ATOM 1242 N THR C 10 16.890 21.717 10.155 1.0019.30 7 N ATOM 1243 CA THR C 10 16.411 20.364 10.393 1.00 17.92 6 C ATOM1244 CB THR C 10 16.843 19.661 11.672 1.00 16.38 6 C ATOM 1245 OG1 THR C10 16.133 20.315 12.741 1.00 18.11 8 O ATOM 1246 CG2 THR C 10 18.34019.783 11.879 1.00 18.62 6 C ATOM 1247 C THR C 10 14.873 20.411 10.4781.00 19.30 6 C ATOM 1248 O THR C 10 14.321 21.487 10.541 1.00 18.20 8 OATOM 1249 N ALA C 11 14.222 19.244 10.430 1.00 19.87 7 N ATOM 1250 CAALA C 11 12.729 19.379 10.557 1.00 21.46 6 C ATOM 1251 CB ALA C 1112.177 17.984 10.450 1.00 20.40 6 C ATOM 1252 C ALA C 11 12.554 20.02411.947 1.00 22.53 6 C ATOM 1253 O ALA C 11 13.387 19.737 12.828 1.0020.28 8 O ATOM 1254 N ALA C 12 11.570 20.893 12.172 1.00 22.83 7 N ATOM1255 CA ALA C 12 11.388 21.485 13.491 1.00 21.67 6 C ATOM 1256 CB ALA C12 10.050 22.226 13.511 1.00 21.82 6 C ATOM 1257 C ALA C 12 11.27920.386 14.546 1.00 22.13 6 C ATOM 1258 O ALA C 12 10.431 19.494 14.3871.00 21.92 8 O ATOM 1259 N GLY C 13 12.059 20.441 15.634 1.00 21.75 7 NATOM 1260 CA GLY C 13 11.867 19.456 16.708 1.00 20.46 6 C ATOM 1261 CGLY C 13 12.824 18.291 16.688 1.00 20.35 6 C ATOM 1262 O GLY C 13 12.99417.498 17.614 1.00 21.82 8 O ATOM 1263 N LYS C 14 13.508 18.073 15.5811.00 21.21 7 N ATOM 1264 CA LYS C 14 14.496 17.032 15.406 1.00 20.28 6 CATOM 1265 CB LYS C 14 14.312 16.435 13.999 1.00 22.02 6 C ATOM 1266 CGLYS C 14 12.877 15.929 13.752 1.00 23.94 6 C ATOM 1267 CD LYS C 1412.495 14.817 14.711 1.00 24.03 6 C ATOM 1268 CE LYS C 14 11.142 14.23314.252 1.00 27.62 6 C ATOM 1269 NZ LYS C 14 10.815 13.033 15.090 1.0029.36 7 N ATOM 1270 C LYS C 14 15.931 17.544 15.561 1.00 20.24 6 C ATOM1271 O LYS C 14 16.810 16.665 15.603 1.00 20.05 8 O ATOM 1272 N GLU C 1516.201 18.853 15.711 1.00 17.96 7 N ATOM 1273 CA GLU C 15 17.600 19.25815.842 1.00 17.39 6 C ATOM 1274 CB GLU C 15 17.723 20.771 16.040 1.0018.15 6 C ATOM 1275 CG GLU C 15 16.929 21.378 17.155 1.00 21.05 6 C ATOM1276 CD GLU C 15 15.446 21.691 16.877 1.00 23.06 6 C ATOM 1277 OE1 GLU C15 14.902 22.458 17.720 1.00 22.27 8 O ATOM 1278 OE2 GLU C 15 14.81121.237 15.899 1.00 19.49 8 O ATOM 1279 C GLU C 15 18.439 18.541 16.8751.00 17.98 6 C ATOM 1280 O GLU C 15 19.633 18.251 16.612 1.00 17.01 8 OATOM 1281 N ARG C 16 17.951 18.262 18.084 1.00 19.93 7 N ATOM 1282 CAARG C 16 18.758 17.602 19.095 1.00 23.34 6 C ATOM 1283 CB ARG C 1618.142 17.638 20.485 1.00 26.49 6 C ATOM 1284 CG ARG C 16 18.577 18.83621.313 1.00 32.57 6 C ATOM 1285 CD ARG C 16 17.739 18.911 22.588 1.0036.19 6 C ATOM 1286 NE ARG C 16 17.646 20.313 23.027 1.00 40.59 7 N ATOM1287 CZ ARG C 16 16.894 20.698 24.064 1.00 43.15 6 C ATOM 1288 NH1 ARG C16 16.218 19.820 24.813 1.00 45.02 7 N ATOM 1289 NH2 ARG C 16 16.88221.992 24.344 1.00 43.41 7 N ATOM 1290 C ARG C 16 19.121 16.155 18.7491.00 23.41 6 C ATOM 1291 O ARG C 16 20.244 15.713 19.020 1.00 24.50 8 OATOM 1292 N GLU C 17 18.196 15.378 18.198 1.00 22.54 7 N ATOM 1293 CAGLU C 17 18.534 13.994 17.878 1.00 22.99 6 C ATOM 1294 CB GLU C 1717.267 13.167 17.843 1.00 23.42 6 C ATOM 1295 CG GLU C 17 16.247 13.46916.765 1.00 26.37 6 C ATOM 1296 CD GLU C 17 16.705 12.793 15.478 1.0029.84 6 C ATOM 1297 OE1 GLU C 17 17.540 11.856 15.651 1.00 30.85 8 OATOM 1298 OE2 GLU C 17 16.264 13.213 14.403 1.00 30.82 8 O ATOM 1299 CGLU C 17 19.392 13.983 16.617 1.00 21.91 6 C ATOM 1300 O GLU C 17 20.20113.066 16.406 1.00 23.15 8 O ATOM 1301 N VAL C 18 19.222 14.931 15.6861.00 20.55 7 N ATOM 1302 CA VAL C 18 20.142 14.929 14.546 1.00 18.68 6 CATOM 1303 CB VAL C 18 19.763 16.002 13.524 1.00 18.86 6 C ATOM 1304 CG1VAL C 18 20.916 16.280 12.556 1.00 17.78 6 C ATOM 1305 CG2 VAL C 1818.534 15.567 12.751 1.00 17.95 6 C ATOM 1306 C VAL C 18 21.529 15.20015.104 1.00 18.89 6 C ATOM 1307 O VAL C 18 22.532 14.523 14.868 1.0020.52 8 O ATOM 1308 N MET C 19 21.637 16.181 15.993 1.00 18.99 7 N ATOM1309 CA MET C 19 22.925 16.541 16.559 1.00 19.72 6 C ATOM 1310 CB MET C19 22.769 17.748 17.479 1.00 19.34 6 C ATOM 1311 CG MET C 19 24.11218.134 18.123 1.00 19.03 6 C ATOM 1312 SD MET C 19 23.905 19.552 19.1721.00 19.97 16 S ATOM 1313 CE MET C 19 22.982 18.859 20.519 1.00 21.11 6C ATOM 1314 C MET C 19 23.606 15.412 17.319 1.00 20.76 6 C ATOM 1315 OMET C 19 24.841 15.407 17.408 1.00 20.12 8 O ATOM 1316 N GLU C 20 22.85614.570 18.009 1.00 21.72 7 N ATOM 1317 CA GLU C 20 23.500 13.493 18.8051.00 22.57 6 C ATOM 1318 CB GLU C 20 22.492 12.946 19.823 1.00 23.97 6 CATOM 1319 CG GLU C 20 22.242 13.984 20.941 1.00 26.93 6 C ATOM 1320 CDGLU C 20 23.506 14.388 21.687 1.00 28.95 6 C ATOM 1321 OE1 GLU C 2024.338 13.471 21.927 1.00 29.90 8 O ATOM 1322 OE2 GLU C 20 23.742 15.58922.041 1.00 29.95 8 O ATOM 1323 C GLU C 20 24.055 12.461 17.840 1.0021.95 6 C ATOM 1324 O GLU C 20 25.107 11.816 18.043 1.00 23.26 8 O ATOM1325 N LYS C 21 23.317 12.251 16.749 1.00 19.49 7 N ATOM 1326 CA LYS C21 23.826 11.337 15.739 1.00 19.77 6 C ATOM 1327 CB LYS C 21 22.81311.186 14.594 1.00 19.76 6 C ATOM 1328 CG LYS C 21 21.640 10.325 15.0931.00 21.20 6 C ATOM 1329 CD LYS C 21 20.679 10.149 13.933 1.00 22.36 6 CATOM 1330 CE LYS C 21 19.385 9.482 14.443 1.00 24.72 6 C ATOM 1331 NZLYS C 21 18.396 9.485 13.330 1.00 25.52 7 N ATOM 1332 C LYS C 21 25.11711.882 15.155 1.00 20.89 6 C ATOM 1333 O LYS C 21 26.020 11.102 14.8161.00 22.01 8 O ATOM 1334 N LEU C 22 25.168 13.196 14.848 1.00 20.08 7 NATOM 1335 CA LEU C 22 26.349 13.828 14.265 1.00 17.68 6 C ATOM 1336 CBLEU C 22 26.026 15.282 13.809 1.00 17.13 6 C ATOM 1337 CG LEU C 2225.157 15.440 12.582 1.00 17.15 6 C ATOM 1338 CD1 LEU C 22 24.528 16.85112.515 1.00 19.16 6 C ATOM 1339 CD2 LEU C 22 25.864 15.262 11.272 1.0018.59 6 C ATOM 1340 C LEU C 22 27.497 13.777 15.240 1.00 18.53 6 C ATOM1341 O LEU C 22 28.660 13.456 14.925 1.00 18.17 8 O ATOM 1342 N LEU C 2327.270 14.064 16.528 1.00 19.42 7 N ATOM 1343 CA LEU C 23 28.369 13.96217.481 1.00 19.76 6 C ATOM 1344 CB LEU C 23 27.928 14.386 18.870 1.0019.18 6 C ATOM 1345 CG LEU C 23 27.619 15.872 18.970 1.00 19.72 6 C ATOM1346 CD1 LEU C 23 26.733 16.088 20.201 1.00 19.30 6 C ATOM 1347 CD2 LEUC 23 28.940 16.627 19.057 1.00 20.29 6 C ATOM 1348 C LEU C 23 28.92112.547 17.596 1.00 20.32 6 C ATOM 1349 O LEU C 23 29.996 12.403 18.2171.00 24.12 8 O ATOM 1350 N ALA C 24 28.288 11.498 17.091 1.00 21.26 7 NATOM 1351 CA ALA C 24 28.945 10.187 17.254 1.00 23.37 6 C ATOM 1352 CBALA C 24 27.913 9.105 17.455 1.00 22.08 6 C ATOM 1353 C ALA C 24 29.9099.985 16.096 1.00 23.91 6 C ATOM 1354 O ALA C 24 30.794 9.135 16.1811.00 26.64 8 O ATOM 1355 N MET C 25 29.831 10.763 15.018 1.00 22.85 7 NATOM 1356 CA MET C 25 30.719 10.545 13.862 1.00 19.77 6 C ATOM 1357 CBMET C 25 30.066 11.120 12.615 1.00 20.26 6 C ATOM 1358 CG MET C 2528.610 10.697 12.416 1.00 18.66 6 C ATOM 1359 SD MET C 25 27.853 11.63211.049 1.00 20.09 16 S ATOM 1360 CE MET C 25 26.186 10.959 11.067 1.0018.76 6 C ATOM 1361 C MET C 25 32.084 11.124 14.126 1.00 20.66 6 C ATOM1362 O MET C 25 32.191 12.215 14.649 1.00 20.74 8 O ATOM 1363 N PRO C 2633.161 10.396 13.787 1.00 19.82 7 N ATOM 1364 CD PRO C 26 33.098 9.04413.168 1.00 20.83 6 C ATOM 1365 CA PRO C 26 34.498 10.816 14.024 1.0019.76 6 C ATOM 1366 CB PRO C 26 35.376 9.630 13.588 1.00 19.77 6 C ATOM1367 CG PRO C 26 34.515 8.779 12.738 1.00 20.30 6 C ATOM 1368 C PRO C 2634.873 12.109 13.315 1.00 17.68 6 C ATOM 1369 O PRO C 26 35.713 12.83813.852 1.00 17.43 8 O ATOM 1370 N GLU C 27 34.287 12.423 12.176 1.0017.43 7 N ATOM 1371 CA GLU C 27 34.610 13.635 11.418 1.00 16.36 6 C ATOM1372 CB GLU C 27 33.964 13.666 10.045 1.00 18.01 6 C ATOM 1373 CG GLU C27 33.994 12.573 9.040 1.00 21.06 6 C ATOM 1374 CD GLU C 27 33.46011.209 9.439 1.00 24.48 6 C ATOM 1375 OE1 GLU C 27 32.905 10.975 10.5441.00 22.13 8 O ATOM 1376 OE2 GLU C 27 33.612 10.328 8.548 1.00 26.95 8 OATOM 1377 C GLU C 27 34.072 14.894 12.132 1.00 15.77 6 C ATOM 1378 O GLUC 27 34.608 16.018 12.001 1.00 13.07 8 O ATOM 1379 N VAL C 28 32.98514.693 12.889 1.00 14.39 7 N ATOM 1380 CA VAL C 28 32.385 15.833 13.5821.00 14.23 6 C ATOM 1381 CB VAL C 28 30.911 15.621 13.946 1.00 13.36 6 CATOM 1382 CG1 VAL C 28 30.347 16.905 14.607 1.00 13.52 6 C ATOM 1383 CG2VAL C 28 30.089 15.300 12.718 1.00 11.93 6 C ATOM 1384 C VAL C 28 33.22416.259 14.766 1.00 14.33 6 C ATOM 1385 O VAL C 28 33.347 15.476 15.7221.00 15.29 8 O ATOM 1386 N LYS C 29 33.694 17.504 14.806 1.00 13.37 7 NATOM 1387 CA LYS C 29 34.438 17.954 15.988 1.00 13.98 6 C ATOM 1388 CBLYS C 29 35.625 18.884 15.662 1.00 13.92 6 C ATOM 1389 CG LYS C 2936.534 18.189 14.630 1.00 16.47 6 C ATOM 1390 CD LYS C 29 36.875 16.76115.038 1.00 16.96 6 C ATOM 1391 CE LYS C 29 38.221 16.374 14.398 1.0022.04 6 C ATOM 1392 NZ LYS C 29 38.262 14.911 14.030 1.00 24.30 7 N ATOM1393 C LYS C 29 33.640 18.713 17.033 1.00 14.44 6 C ATOM 1394 O LYS C 2934.018 18.680 18.202 1.00 12.53 8 O ATOM 1395 N GLU C 30 32.583 19.41916.575 1.00 13.94 7 N ATOM 1396 CA GLU C 30 31.766 20.156 17.551 1.0013.46 6 C ATOM 1397 CB GLU C 30 32.435 21.389 18.153 1.00 14.91 6 C ATOM1398 CG GLU C 30 32.868 22.376 17.121 1.00 17.59 6 C ATOM 1399 CD GLU C30 33.308 23.755 17.492 1.00 18.48 6 C ATOM 1400 OE1 GLU C 30 33.81824.439 16.555 1.00 19.49 8 O ATOM 1401 OE2 GLU C 30 33.093 24.296 18.5871.00 18.98 8 O ATOM 1402 C GLU C 30 30.423 20.378 16.853 1.00 12.44 6 CATOM 1403 O GLU C 30 30.303 20.285 15.629 1.00 10.96 8 O ATOM 1404 N ALAC 31 29.366 20.505 17.626 1.00 11.16 7 N ATOM 1405 CA ALA C 31 28.02120.599 17.048 1.00 10.93 6 C ATOM 1406 CB ALA C 31 27.428 19.201 16.8221.00 10.63 6 C ATOM 1407 CA ALA C 31 27.190 21.315 18.113 1.00 10.97 6 CATOM 1408 O ALA C 31 27.360 21.054 19.316 1.00 9.38 8 O ATOM 1409 N TYRC 32 26.391 22.275 17.649 1.00 9.53 7 N ATOM 1410 CA TYR C 32 25.53023.025 18.552 1.00 8.67 6 C ATOM 1411 CB TYR C 32 26.086 24.395 18.8911.00 9.25 6 C ATOM 1412 CG TYR C 32 27.408 24.481 19.615 1.00 10.08 6 CATOM 1413 CD1 TYR C 32 27.357 24.671 20.988 1.00 11.88 6 C ATOM 1414 CE1TYR C 32 28.503 24.740 21.794 1.00 12.28 6 C ATOM 1415 CD2 TYR C 3228.646 24.324 18.992 1.00 10.95 6 C ATOM 1416 CE2 TYR C 32 29.799 24.40319.764 1.00 11.49 6 C ATOM 1417 CZ TYR C 32 29.709 24.623 21.121 1.0012.94 6 C ATOM 1418 OH TYR C 32 30.827 24.777 21.921 1.00 15.26 8 O ATOM1419 C TYR C 32 24.204 23.305 17.843 1.00 10.59 6 C ATOM 1420 O TYR C 3224.121 23.444 16.603 1.00 10.55 8 O ATOM 1421 N VAL C 33 23.162 23.33618.661 1.00 9.72 7 N ATOM 1422 CA VAL C 33 21.851 23.736 18.161 1.009.11 6 C ATOM 1423 CB VAL C 33 20.683 23.161 18.968 1.00 9.44 6 C ATOM1424 CG1 VAL C 33 19.379 23.787 18.505 1.00 11.05 6 C ATOM 1425 CG2 VALC 33 20.646 21.639 18.742 1.00 9.65 6 C ATOM 1426 C VAL C 33 21.84525.259 18.352 1.00 8.78 6 C ATOM 1427 O VAL C 33 22.349 25.739 19.3681.00 11.19 8 O ATOM 1428 N VAL C 34 21.478 26.012 17.342 1.00 8.87 7 NATOM 1429 CA VAL C 34 21.543 27.472 17.359 1.00 7.42 6 C ATOM 1430 CBVAL C 34 22.674 27.896 16.409 1.00 7.82 6 C ATOM 1431 CG1 VAL C 3424.065 27.329 16.849 1.00 6.17 6 C ATOM 1432 CG2 VAL C 34 22.466 27.45714.933 1.00 5.87 6 C ATOM 1433 C VAL C 34 20.225 28.140 16.954 1.00 9.546 C ATOM 1434 O VAL C 34 19.395 27.580 16.223 1.00 8.27 8 O ATOM 1435 NTYR C 35 20.027 29.392 17.383 1.00 10.13 7 N ATOM 1436 CA TYR C 3518.976 30.284 16.947 1.00 11.95 6 C ATOM 1437 CB TYR C 35 18.775 31.48317.876 1.00 12.68 6 C ATOM 1438 CG TYR C 35 18.185 31.039 19.195 1.0017.52 6 C ATOM 1439 CD1 TYR C 35 16.816 31.015 19.360 1.00 18.77 6 CATOM 1440 CE1 TYR C 35 16.271 30.631 20.564 1.00 18.30 6 C ATOM 1441 CD2TYR C 35 18.994 30.662 20.262 1.00 17.66 6 C ATOM 1442 CE2 TYR C 3518.444 30.266 21.460 1.00 18.18 6 C ATOM 1443 CZ TYR C 35 17.067 30.26121.601 1.00 18.43 6 C ATOM 1444 OH TYR C 35 16.493 29.868 22.789 1.0018.93 8 O ATOM 1445 C TYR C 35 19.300 30.955 15.604 1.00 12.91 6 C ATOM1446 O TYR C 35 20.482 31.218 15.319 1.00 12.84 8 O ATOM 1447 N GLY C 3618.299 31.196 14.782 1.00 14.24 7 N ATOM 1448 CA GLY C 36 18.520 31.85913.478 1.00 12.60 6 C ATOM 1449 C GLY C 36 17.864 31.056 12.389 1.0014.26 6 C ATOM 1450 O GLY C 36 17.106 30.079 12.649 1.00 18.80 8 O ATOM1451 N GLU C 37 18.194 31.299 11.145 1.00 18.17 7 N ATOM 1452 CA GLU C37 17.633 30.564 10.002 1.00 23.09 6 C ATOM 1453 CB GLU C 37 18.16931.203 8.714 1.00 27.51 6 C ATOM 1454 CG GLU C 37 17.650 30.595 7.4071.00 32.04 6 C ATOM 1455 CD GLU C 37 18.546 31.061 6.256 1.00 35.48 6 CATOM 1456 OE1 GLU C 37 19.323 30.257 5.686 1.00 37.55 8 O ATOM 1457 OE2GLU C 37 18.521 32.254 5.909 1.00 36.51 8 O ATOM 1458 C GLU C 37 18.06629.104 10.001 1.00 23.63 6 C ATOM 1459 O GLU C 37 17.322 28.198 9.6181.00 23.98 8 O ATOM 1460 N TYR C 38 19.316 28.847 10.403 1.00 21.56 7 NATOM 1461 CA TYR C 38 19.836 27.509 10.574 1.00 19.52 6 C ATOM 1462 CBTYR C 38 21.368 27.543 10.441 1.00 22.39 6 C ATOM 1463 CG TYR C 3821.703 28.026 9.039 1.00 24.37 6 C ATOM 1464 CD1 TYR C 38 21.292 27.2587.969 1.00 25.06 6 C ATOM 1465 CE1 TYR C 38 21.584 27.690 6.693 1.0028.43 6 C ATOM 1466 CD2 TYR C 38 22.404 29.209 8.861 1.00 25.04 6 C ATOM1467 CE2 TYR C 38 22.726 29.599 7.572 1.00 27.79 6 C ATOM 1468 CZ TYR C38 22.292 28.843 6.501 1.00 27.61 6 C ATOM 1469 OH TYR C 38 22.59629.243 5.230 1.00 31.14 8 O ATOM 1470 C TYR C 38 19.566 27.040 12.0101.00 17.33 6 C ATOM 1471 O TYR C 38 19.308 27.808 12.915 1.00 18.03 8 OATOM 1472 N ASP C 39 19.479 25.752 12.164 1.00 15.50 7 N ATOM 1473 CAASP C 39 19.069 25.122 13.403 1.00 16.04 6 C ATOM 1474 CB ASP C 3918.092 24.006 13.020 1.00 14.43 6 C ATOM 1475 CG ASP C 39 16.840 24.56612.407 1.00 16.60 6 C ATOM 1476 OD1 ASP C 39 16.453 25.687 12.752 1.0015.98 8 O ATOM 1477 OD2 ASP C 39 16.250 23.864 11.558 1.00 20.27 8 OATOM 1478 C ASP C 39 20.282 24.495 14.086 1.00 13.53 6 C ATOM 1479 O ASPC 39 20.326 24.344 15.307 1.00 14.94 8 O ATOM 1480 N LEU C 40 21.23224.154 13.194 1.00 12.61 7 N ATOM 1481 CA LEU C 40 22.439 23.489 13.6891.00 13.53 6 C ATOM 1482 CB LEU C 40 22.190 22.022 13.285 1.00 15.55 6 CATOM 1483 CG LEU C 40 22.710 20.831 14.001 1.00 19.18 6 C ATOM 1484 CD1LEU C 40 22.457 20.879 15.503 1.00 19.65 6 C ATOM 1485 CD2 LEU C 4021.942 19.557 13.514 1.00 18.15 6 C ATOM 1486 C LEU C 40 23.716 23.92812.995 1.00 11.96 6 C ATOM 1487 O LEU C 40 23.749 24.157 11.769 1.0010.54 8 O ATOM 1488 N ILE C 41 24.785 23.932 13.784 1.00 11.74 7 N ATOM1489 CA ILE C 41 26.123 24.205 13.183 1.00 11.84 6 C ATOM 1490 CB ILE C41 26.679 25.588 13.482 1.00 12.75 6 C ATOM 1491 CG2 ILE C 41 26.69925.790 14.971 1.00 13.88 6 C ATOM 1492 CG1 ILE C 41 28.120 25.681 12.8751.00 13.00 6 C ATOM 1493 CD1 ILE C 41 28.527 27.134 12.744 1.00 14.55 6C ATOM 1494 C ILE C 41 27.072 23.118 13.664 1.00 10.85 6 C ATOM 1495 OILE C 41 27.076 22.742 14.846 1.00 10.67 8 O ATOM 1496 N VAL C 42 27.80122.553 12.699 1.00 10.11 7 N ATOM 1497 CA VAL C 42 28.738 21.454 12.9641.00 11.25 6 C ATOM 1498 CB VAL C 42 28.087 20.228 12.232 1.00 14.86 6 CATOM 1499 CG1 VAL C 42 28.985 19.118 11.842 1.00 13.65 6 C ATOM 1500 CG2VAL C 42 26.998 19.628 13.169 1.00 15.56 6 C ATOM 1501 C VAL C 42 30.08421.759 12.362 1.00 11.05 6 C ATOM 1502 O VAL C 42 30.168 22.412 11.3001.00 11.42 8 O ATOM 1503 N LYS C 43 31.164 21.525 13.084 1.00 11.17 7 NATOM 1504 CA LYS C 43 32.510 21.655 12.560 1.00 10.83 6 C ATOM 1505 CBLYS C 43 33.423 22.361 13.542 1.00 10.74 6 C ATOM 1506 CG LYS C 4334.834 22.632 12.996 1.00 12.24 6 C ATOM 1507 CD LYS C 43 35.626 22.99214.268 1.00 15.84 6 C ATOM 1508 CE LYS C 43 37.101 23.230 14.123 1.0015.74 6 C ATOM 1509 NZ LYS C 43 37.562 23.826 15.456 1.00 14.56 7 N ATOM1510 C LYS C 43 33.051 20.251 12.241 1.00 11.80 6 C ATOM 1511 O LYS C 4333.135 19.345 13.083 1.00 10.83 8 O ATOM 1512 N VAL C 44 33.453 20.04210.989 1.00 11.36 7 N ATOM 1513 CA VAL C 44 33.934 18.777 10.453 1.0011.94 6 C ATOM 1514 CB VAL C 44 32.975 18.306 9.341 1.00 13.11 6 C ATOM1515 CG1 VAL C 44 33.430 17.009 8.657 1.00 16.19 6 C ATOM 1516 CG2 VAL C44 31.583 17.978 9.900 1.00 13.88 6 C ATOM 1517 C VAL C 44 35.387 18.93410.004 1.00 13.35 6 C ATOM 1518 O VAL C 44 35.899 20.015 9.598 1.0013.28 8 O ATOM 1519 N GLU C 45 36.207 17.906 10.191 1.00 14.07 7 N ATOM1520 CA GLU C 45 37.622 17.831 9.808 1.00 14.76 6 C ATOM 1521 CB GLU C45 38.680 18.063 10.878 1.00 16.30 6 C ATOM 1522 CG GLU C 45 38.63119.447 11.511 1.00 19.96 6 C ATOM 1523 CD GLU C 45 39.528 19.670 12.6981.00 22.48 6 C ATOM 1524 OE1 GLU C 45 39.541 20.843 13.173 1.00 25.62 8O ATOM 1525 OE2 GLU C 45 40.204 18.732 13.168 1.00 22.11 8 O ATOM 1526 CGLU C 45 37.840 16.458 9.161 1.00 16.36 6 C ATOM 1527 O GLU C 45 37.10615.509 9.540 1.00 15.71 8 O ATOM 1528 N THR C 46 38.215 16.526 7.8571.00 16.74 7 N ATOM 1529 CA THR C 46 38.348 15.349 7.020 1.00 16.32 6 CATOM 1530 CB THR C 46 37.338 15.094 5.916 1.00 17.33 6 C ATOM 1531 OG1THR C 46 37.480 16.090 4.875 1.00 17.34 8 O ATOM 1532 CG2 THR C 4635.887 15.114 6.379 1.00 18.83 6 C ATOM 1533 C THR C 46 39.742 15.4136.391 1.00 16.88 6 C ATOM 1534 O THR C 46 40.446 16.425 6.502 1.00 16.938 O ATOM 1535 N ASP C 47 40.290 14.231 6.023 1.00 19.87 7 N ATOM 1536 CAASP C 47 41.700 14.317 5.576 1.00 21.73 6 C ATOM 1537 CB ASP C 47 42.42612.954 5.586 1.00 26.37 6 C ATOM 1538 CG ASP C 47 41.716 12.020 4.6301.00 29.72 6 C ATOM 1539 OD1 ASP C 47 40.591 12.393 4.229 1.00 31.09 8 OATOM 1540 OD2 ASP C 47 42.171 10.932 4.213 1.00 33.95 8 O ATOM 1541 CASP C 47 41.738 14.992 4.194 1.00 19.55 6 C ATOM 1542 O ASP C 47 42.63415.814 4.039 1.00 20.55 8 O ATOM 1543 N THR C 48 40.768 14.797 3.3131.00 17.36 7 N ATOM 1544 CA THR C 48 40.777 15.364 1.972 1.00 16.80 6 CATOM 1545 CB THR C 48 40.998 14.279 0.889 1.00 17.66 6 C ATOM 1546 OG1THR C 48 39.843 13.418 0.841 1.00 19.76 8 O ATOM 1547 CG2 THR C 4842.226 13.399 1.066 1.00 18.46 6 C ATOM 1548 C THR C 48 39.390 15.9621.645 1.00 17.80 6 C ATOM 1549 O THR C 48 38.410 15.767 2.371 1.00 17.968 O ATOM 1550 N LEU C 49 39.276 16.693 0.539 1.00 17.38 7 N ATOM 1551 CALEU C 49 38.041 17.313 0.104 1.00 17.64 6 C ATOM 1552 CB LEU C 49 38.37318.361 −0.982 1.00 18.40 6 C ATOM 1553 CG LEU C 49 37.081 19.124 −1.3861.00 19.66 6 C ATOM 1554 CD1 LEU C 49 36.587 19.885 −0.153 1.00 18.61 6C ATOM 1555 CD2 LEU C 49 37.331 20.095 −2.523 1.00 20.55 6 C ATOM 1556 CLEU C 49 37.073 16.254 −0.415 1.00 19.38 6 C ATOM 1557 O LEU C 49 35.85416.268 −0.227 1.00 17.37 8 O ATOM 1558 N LYS C 50 37.667 15.245 −1.0851.00 19.62 7 N ATOM 1559 CA LYS C 50 36.914 14.069 −1.516 1.00 22.17 6 CATOM 1560 CB LYS C 50 37.782 13.055 −2.251 1.00 25.37 6 C ATOM 1561 CGLYS C 50 37.245 11.638 −2.416 1.00 29.73 6 C ATOM 1562 CD LYS C 5038.410 10.633 −2.435 1.00 32.69 6 C ATOM 1563 CE LYS C 50 38.020 9.201−2.769 1.00 34.00 6 C ATOM 1564 NZ LYS C 50 38.205 8.880 −4.230 1.0036.40 7 N ATOM 1565 C LYS C 50 36.286 13.404 −0.280 1.00 19.70 6 C ATOM1566 O LYS C 50 35.097 13.147 −0.311 1.00 18.54 8 O ATOM 1567 N ASP C 5137.028 13.195 0.810 1.00 20.24 7 N ATOM 1568 CA ASP C 51 36.392 12.6642.031 1.00 19.47 6 C ATOM 1569 CB ASP C 51 37.475 12.383 3.087 1.0020.84 6 C ATOM 1570 CG ASP C 51 38.327 11.194 2.601 1.00 22.11 6 C ATOM1571 OD1 ASP C 51 37.801 10.426 1.779 1.00 22.72 8 O ATOM 1572 OD2 ASP C51 39.478 11.066 3.025 1.00 20.72 8 O ATOM 1573 C ASP C 51 35.320 13.5732.580 1.00 19.16 6 C ATOM 1574 O ASP C 51 34.292 13.068 3.019 1.00 21.048 O ATOM 1575 N LEU C 52 35.448 14.912 2.479 1.00 18.94 7 N ATOM 1576 CALEU C 52 34.381 15.799 2.945 1.00 15.62 6 C ATOM 1577 CB LEU C 52 34.81317.271 2.792 1.00 15.17 6 C ATOM 1578 CG LEU C 52 33.760 18.354 3.0781.00 12.83 6 C ATOM 1579 CD1 LEU C 52 33.238 18.194 4.507 1.00 14.01 6 CATOM 1580 CD2 LEU C 52 34.453 19.713 2.883 1.00 13.45 6 C ATOM 1581 CLEU C 52 33.132 15.606 2.142 1.00 16.81 6 C ATOM 1582 O LEU C 52 32.02615.503 2.644 1.00 16.79 8 O ATOM 1583 N ASP C 53 33.296 15.652 0.8111.00 20.54 7 N ATOM 1584 CA ASP C 53 32.156 15.528 −0.100 1.00 23.66 6 CATOM 1585 CB ASP C 53 32.600 15.643 −1.558 1.00 24.36 6 C ATOM 1586 CGASP C 53 31.433 15.675 −2.527 1.00 27.18 6 C ATOM 1587 OD1 ASP C 5330.847 14.603 −2.831 1.00 28.04 8 O ATOM 1588 OD2 ASP C 53 31.048 16.750−3.064 1.00 28.27 8 O ATOM 1589 C ASP C 53 31.465 14.198 0.183 1.0024.54 6 C ATOM 1590 O ASP C 53 30.237 14.171 0.266 1.00 27.34 8 O ATOM1591 N GLN C 54 32.212 13.126 0.480 1.00 25.41 7 N ATOM 1592 CA GLN C 5431.528 11.856 0.704 1.00 27.72 6 C ATOM 1593 CB GLN C 54 32.540 10.7260.543 1.00 29.95 6 C ATOM 1594 CG GLN C 54 31.771 9.421 0.229 1.00 35.886 C ATOM 1595 CD GLN C 54 32.776 8.260 0.234 1.00 38.59 6 C ATOM 1596OE1 GLN C 54 33.997 8.535 0.208 1.00 40.18 8 O ATOM 1597 NE2 GLN C 5432.222 7.057 0.258 1.00 38.68 7 N ATOM 1598 C GLN C 54 30.861 11.7582.073 1.00 26.83 6 C ATOM 1599 O GLN C 54 29.910 10.973 2.244 1.00 27.408 O ATOM 1600 N PHE C 55 31.350 12.565 3.019 1.00 23.70 7 N ATOM 1601 CAPHE C 55 30.710 12.620 4.348 1.00 21.86 6 C ATOM 1602 CB PHE C 55 31.70213.193 5.357 1.00 19.93 6 C ATOM 1603 CG PHE C 55 30.987 13.609 6.6141.00 20.88 6 C ATOM 1604 CD1 PHE C 55 30.884 12.742 7.683 1.00 19.06 6 CATOM 1605 CD2 PHE C 55 30.394 14.873 6.690 1.00 19.80 6 C ATOM 1606 CE1PHE C 55 30.209 13.115 8.823 1.00 17.49 6 C ATOM 1607 CE2 PHE C 5529.715 15.249 7.830 1.00 20.56 6 C ATOM 1608 CZ PHE C 55 29.643 14.3528.895 1.00 19.05 6 C ATOM 1609 C PHE C 55 29.387 13.339 4.235 1.00 21.616 C ATOM 1610 O PHE C 55 28.353 12.900 4.766 1.00 22.20 8 O ATOM 1611 NILE C 56 29.352 14.429 3.454 1.00 21.99 7 N ATOM 1612 CA ILE C 56 28.09215.134 3.220 1.00 22.47 6 C ATOM 1613 CB ILE C 56 28.328 16.386 2.3371.00 22.35 6 C ATOM 1614 CG2 ILE C 56 27.039 16.904 1.709 1.00 21.09 6 CATOM 1615 CG1 ILE C 56 29.004 17.487 3.171 1.00 21.32 6 C ATOM 1616 CD1ILE C 56 29.601 18.543 2.264 1.00 24.85 6 C ATOM 1617 C ILE C 56 27.04414.280 2.523 1.00 24.70 6 C ATOM 1618 O ILE C 56 25.851 14.393 2.8391.00 26.85 8 O ATOM 1619 N THR C 57 27.403 13.577 1.446 1.00 26.48 7 NATOM 1620 CA THR C 57 26.407 12.847 0.667 1.00 27.16 6 C ATOM 1621 CBTHR C 57 27.007 12.425 −0.687 1.00 27.37 6 C ATOM 1622 OG1 THR C 5728.236 11.757 −0.326 1.00 30.53 8 O ATOM 1623 CG2 THR C 57 27.367 13.571−1.616 1.00 25.93 6 C ATOM 1624 C THR C 57 25.977 11.580 1.383 1.0029.79 6 C ATOM 1625 O THR C 57 24.792 11.181 1.373 1.00 32.61 8 O ATOM1626 N GLU C 58 26.951 10.884 1.965 1.00 30.00 7 N ATOM 1627 CA GLU C 5826.591 9.627 2.622 1.00 31.50 6 C ATOM 1628 CB GLU C 58 27.826 8.7472.774 1.00 33.53 6 C ATOM 1629 CG GLU C 58 28.512 8.540 1.428 1.00 36.716 C ATOM 1630 CD GLU C 58 27.648 8.445 0.182 1.00 39.49 6 C ATOM 1631OE1 GLU C 58 26.556 7.813 0.197 1.00 39.86 8 O ATOM 1632 OE2 GLU C 5828.074 8.990 −0.885 1.00 40.22 8 O ATOM 1633 C GLU C 58 25.848 9.9213.911 1.00 31.88 6 C ATOM 1634 O GLU C 58 24.713 9.432 4.067 1.00 32.248 O ATOM 1635 N LYS C 59 26.476 10.754 4.754 1.00 31.30 7 N ATOM 1636 CALYS C 59 25.833 11.012 6.046 1.00 29.92 6 C ATOM 1637 CB LYS C 59 26.89611.140 7.160 1.00 30.17 6 C ATOM 1638 CG LYS C 59 28.102 10.256 6.9781.00 31.15 6 C ATOM 1639 CD LYS C 59 28.432 9.440 8.232 1.00 34.64 6 CATOM 1640 CE LYS C 59 29.922 9.072 8.262 1.00 35.28 6 C ATOM 1641 NZ LYSC 59 30.375 8.487 9.562 1.00 35.48 7 N ATOM 1642 C LYS C 59 24.91412.203 6.056 1.00 28.84 6 C ATOM 1643 O LYS C 59 23.891 12.075 6.7571.00 32.65 8 O ATOM 1644 N ILE C 60 25.105 13.337 5.401 1.00 27.08 7 NATOM 1645 CA ILE C 60 24.205 14.461 5.598 1.00 24.04 6 C ATOM 1646 CBILE C 60 24.917 15.824 5.491 1.00 22.38 6 C ATOM 1647 CG2 ILE C 6023.980 17.028 5.513 1.00 22.26 6 C ATOM 1648 CG1 ILE C 60 25.945 15.9876.614 1.00 22.82 6 C ATOM 1649 CD1 ILE C 60 25.479 15.800 8.043 1.0022.99 6 C ATOM 1650 C ILE C 60 22.982 14.420 4.717 1.00 26.78 6 C ATOM1651 O ILE C 60 21.885 14.876 5.126 1.00 27.54 8 O ATOM 1652 N ARG C 6123.097 13.874 3.518 1.00 27.90 7 N ATOM 1653 CA ARG C 61 21.922 13.8222.639 1.00 28.63 6 C ATOM 1654 CB ARG C 61 22.337 14.115 1.194 1.0028.99 6 C ATOM 1655 CG ARG C 61 22.193 15.571 0.776 1.00 29.41 6 C ATOM1656 CD ARG C 61 23.149 15.907 −0.362 1.00 30.77 6 C ATOM 1657 NE ARG C61 23.058 17.293 −0.839 1.00 30.00 7 N ATOM 1658 CZ ARG C 61 23.82317.717 −1.861 1.00 31.73 6 C ATOM 1659 NH1 ARG C 61 24.650 16.865 −2.5191.00 30.90 7 N ATOM 1660 NH2 ARG C 61 23.747 18.977 −2.334 1.00 31.15 7N ATOM 1661 C ARG C 61 21.144 12.525 2.785 1.00 29.56 6 C ATOM 1662 OARG C 61 20.054 12.488 2.195 1.00 32.69 8 O ATOM 1663 N LYS C 62 21.50711.519 3.565 1.00 28.81 7 N ATOM 1664 CA LYS C 62 20.703 10.354 3.8501.00 28.73 6 C ATOM 1665 CB LYS C 62 21.495 9.038 3.876 1.00 29.41 6 CATOM 1666 CG LYS C 62 21.713 8.469 2.472 1.00 30.20 6 C ATOM 1667 CD LYSC 62 22.852 7.434 2.430 1.00 32.21 6 C ATOM 1668 CE LYS C 62 23.3567.366 0.974 1.00 32.81 6 C ATOM 1669 NZ LYS C 62 24.510 6.429 0.843 1.0033.64 7 N ATOM 1670 C LYS C 62 20.038 10.515 5.230 1.00 30.02 6 C ATOM1671 O LYS C 62 19.651 9.527 5.885 1.00 32.12 8 O ATOM 1672 N MET C 6320.177 11.713 5.823 1.00 28.76 7 N ATOM 1673 CA MET C 63 19.493 11.9807.098 1.00 26.81 6 C ATOM 1674 CB MET C 63 20.435 12.737 8.034 1.0027.75 6 C ATOM 1675 CG MET C 63 20.688 11.900 9.255 1.00 28.92 6 C ATOM1676 SD MET C 63 21.342 12.751 10.713 1.00 34.47 16 S ATOM 1677 CE MET C63 23.079 12.594 10.294 1.00 29.00 6 C ATOM 1678 C MET C 63 18.23112.718 6.738 1.00 25.17 6 C ATOM 1679 O MET C 63 18.220 13.864 6.2711.00 27.77 8 O ATOM 1680 N PRO C 64 17.097 12.006 6.766 1.00 23.96 7 NATOM 1681 CD PRO C 64 16.986 10.619 7.289 1.00 24.17 6 C ATOM 1682 CAPRO C 64 15.808 12.538 6.355 1.00 24.05 6 C ATOM 1683 CB PRO C 64 14.87811.345 6.357 1.00 24.26 6 C ATOM 1684 CG PRO C 64 15.491 10.380 7.3101.00 24.02 6 C ATOM 1685 C PRO C 64 15.351 13.693 7.238 1.00 23.26 6 CATOM 1686 O PRO C 64 14.634 14.599 6.786 1.00 23.48 8 O ATOM 1687 N GLUC 65 15.894 13.773 8.458 1.00 23.82 7 N ATOM 1688 CA GLU C 65 15.50314.946 9.287 1.00 25.38 6 C ATOM 1689 CB GLU C 65 15.737 14.529 10.7421.00 26.46 6 C ATOM 1690 CG GLU C 65 14.861 13.328 11.046 1.00 29.88 6 CATOM 1691 CD GLU C 65 15.581 12.403 11.996 1.00 33.43 6 C ATOM 1692 OE1GLU C 65 14.905 11.607 12.707 1.00 36.06 8 O ATOM 1693 OE2 GLU C 6516.826 12.495 12.168 1.00 34.58 8 O ATOM 1694 C GLU C 65 16.224 16.2288.933 1.00 25.09 6 C ATOM 1695 O GLU C 65 15.716 17.320 9.257 1.00 25.898 O ATOM 1696 N ILE C 66 17.369 16.098 8.233 1.00 23.79 7 N ATOM 1697 CAILE C 66 18.156 17.256 7.814 1.00 22.74 6 C ATOM 1698 CB ILE C 66 19.63516.857 7.670 1.00 22.13 6 C ATOM 1699 CG2 ILE C 66 20.468 17.984 7.0861.00 22.33 6 C ATOM 1700 CG1 ILE C 66 20.165 16.412 9.012 1.00 20.58 6 CATOM 1701 CD1 ILE C 66 21.611 15.995 9.067 1.00 23.30 6 C ATOM 1702 CILE C 66 17.578 17.729 6.511 1.00 24.00 6 C ATOM 1703 O ILE C 66 17.30116.854 5.665 1.00 26.96 8 O ATOM 1704 N GLN C 67 17.361 19.007 6.2831.00 25.25 7 N ATOM 1705 CA GLN C 67 16.593 19.488 5.154 1.00 25.62 6 CATOM 1706 CB GLN C 67 15.371 20.251 5.750 1.00 27.64 6 C ATOM 1707 CGGLN C 67 14.314 19.213 6.197 1.00 30.98 6 C ATOM 1708 CD GLN C 67 13.08219.895 6.738 1.00 32.31 6 C ATOM 1709 OE1 GLN C 67 12.934 21.113 6.5141.00 35.41 8 O ATOM 1710 NE2 GLN C 67 12.264 19.145 7.471 1.00 33.33 7 NATOM 1711 C GLN C 67 17.276 20.434 4.200 1.00 28.28 6 C ATOM 1712 O GLNC 67 16.880 20.489 3.003 1.00 30.74 8 O ATOM 1713 N MET C 68 18.15621.310 4.658 1.00 27.47 7 N ATOM 1714 CA MET C 68 18.862 22.263 3.8021.00 24.68 6 C ATOM 1715 CB MET C 68 18.008 23.515 3.700 1.00 26.70 6 CATOM 1716 CG MET C 68 18.697 24.745 3.118 1.00 31.25 6 C ATOM 1717 SDMET C 68 17.815 26.277 3.584 1.00 38.51 16 S ATOM 1718 CE MET C 6819.212 27.404 3.757 1.00 35.34 6 C ATOM 1719 C MET C 68 20.189 22.6454.477 1.00 23.75 6 C ATOM 1720 O MET C 68 20.099 23.063 5.644 1.00 19.668 O ATOM 1721 N THR C 69 21.313 22.411 3.789 1.00 21.36 7 N ATOM 1722 CATHR C 69 22.618 22.639 4.357 1.00 19.81 6 C ATOM 1723 CB THR C 69 23.40421.315 4.525 1.00 20.43 6 C ATOM 1724 OG1 THR C 69 24.167 21.019 3.3621.00 22.61 8 O ATOM 1725 CG2 THR C 69 22.506 20.131 4.834 1.00 17.73 6 CATOM 1726 C THR C 69 23.490 23.637 3.618 1.00 18.75 6 C ATOM 1727 O THRC 69 23.440 23.819 2.409 1.00 20.19 8 O ATOM 1728 N SER C 70 24.29524.391 4.359 1.00 16.80 7 N ATOM 1729 CA SER C 70 25.258 25.283 3.7401.00 17.93 6 C ATOM 1730 CB SER C 70 24.713 26.687 3.927 1.00 17.55 6 CATOM 1731 OG SER C 70 25.650 27.578 3.348 1.00 22.26 8 O ATOM 1732 C SERC 70 26.657 24.999 4.302 1.00 17.09 6 C ATOM 1733 O SER C 70 26.84725.050 5.520 1.00 17.01 8 O ATOM 1734 N THR C 71 27.600 24.667 3.4231.00 14.94 7 N ATOM 1735 CA THR C 71 28.959 24.328 3.889 1.00 15.34 6 CATOM 1736 CB THR C 71 29.491 23.036 3.200 1.00 15.88 6 C ATOM 1737 OG1THR C 71 28.576 21.980 3.539 1.00 15.96 8 O ATOM 1738 CG2 THR C 7130.856 22.573 3.655 1.00 16.01 6 C ATOM 1739 C THR C 71 29.906 25.4723.588 1.00 14.07 6 C ATOM 1740 O THR C 71 29.908 26.005 2.467 1.00 13.138 O ATOM 1741 N MET C 72 30.733 25.786 4.576 1.00 12.04 7 N ATOM 1742 CAMET C 72 31.806 26.752 4.438 1.00 12.02 6 C ATOM 1743 CB MET C 72 31.61227.963 5.351 1.00 11.60 6 C ATOM 1744 CG MET C 72 30.249 28.638 5.1531.00 17.54 6 C ATOM 1745 SD MET C 72 29.862 29.742 6.517 1.00 18.74 16 SATOM 1746 CE MET C 72 28.292 30.398 6.028 1.00 21.26 6 C ATOM 1747 C METC 72 33.168 26.143 4.823 1.00 11.23 6 C ATOM 1748 O MET C 72 33.47125.902 6.015 1.00 8.34 8 O ATOM 1749 N ILE C 73 33.996 25.961 3.813 1.0010.73 7 N ATOM 1750 CA ILE C 73 35.331 25.404 4.056 1.00 10.84 6 C ATOM1751 CB ILE C 73 35.953 24.995 2.723 1.00 13.41 6 C ATOM 1752 CG2 ILE C73 37.443 24.649 2.885 1.00 12.03 6 C ATOM 1753 CG1 ILE C 73 35.10523.853 2.157 1.00 14.14 6 C ATOM 1754 CD1 ILE C 73 35.758 23.179 0.9541.00 16.09 6 C ATOM 1755 C ILE C 73 36.203 26.456 4.725 1.00 13.62 6 CATOM 1756 O ILE C 73 36.216 27.633 4.359 1.00 13.19 8 O ATOM 1757 N ALAC 74 36.934 26.033 5.735 1.00 14.74 7 N ATOM 1758 CA ALA C 74 37.89626.893 6.398 1.00 18.25 6 C ATOM 1759 CB ALA C 74 37.993 26.485 7.8581.00 18.16 6 C ATOM 1760 C ALA C 74 39.226 26.939 5.669 1.00 22.37 6 CATOM 1761 O ALA C 74 39.598 26.057 4.867 1.00 24.32 8 O ATOM 1762 N ILEC 75 39.770 28.140 5.533 1.00 24.13 7 N ATOM 1763 CA ILE C 75 41.07228.446 4.996 1.00 26.43 6 C ATOM 1764 CB ILE C 75 41.185 29.580 3.9771.00 25.92 6 C ATOM 1765 CG2 ILE C 75 42.611 29.746 3.459 1.00 25.06 6 CATOM 1766 CG1 ILE C 75 40.232 29.433 2.812 1.00 25.87 6 C ATOM 1767 CD1ILE C 75 39.600 30.788 2.473 1.00 25.99 6 C ATOM 1768 C ILE C 75 41.83228.964 6.239 1.00 29.95 6 C ATOM 1769 O ILE C 75 41.645 28.455 7.3931.00 29.32 8 O ATOM 1770 OT ILE C 75 42.313 30.128 6.106 1.00 33.07 8 OATOM 1771 CB VAL D 2 25.991 33.212 26.292 1.00 10.82 6 C ATOM 1772 CG1VAL D 2 25.191 33.260 24.963 1.00 10.54 6 C ATOM 1773 CG2 VAL D 2 24.99833.476 27.435 1.00 11.04 6 C ATOM 1774 C VAL D 2 27.631 31.627 25.3731.00 9.57 6 C ATOM 1775 O VAL D 2 28.621 32.340 25.380 1.00 12.23 8 OATOM 1776 N VAL D 2 27.227 31.645 27.864 1.00 11.94 7 N ATOM 1777 CA VALD 2 26.629 31.841 26.486 1.00 10.20 6 C ATOM 1778 N THR D 3 27.38730.772 24.418 1.00 10.51 7 N ATOM 1779 CA THR D 3 28.201 30.539 23.2331.00 12.03 6 C ATOM 1780 CB THR D 3 28.465 29.034 23.075 1.00 12.43 6 CATOM 1781 OG1 THR D 3 29.142 28.582 24.249 1.00 13.41 8 O ATOM 1782 CG2THR D 3 29.325 28.600 21.892 1.00 9.36 6 C ATOM 1783 C THR D 3 27.45331.041 21.984 1.00 10.73 6 C ATOM 1784 O THR D 3 26.224 30.920 21.8441.00 8.60 8 O ATOM 1785 N ALA D 4 28.180 31.725 21.110 1.00 8.65 7 NATOM 1786 CA ALA D 4 27.522 32.150 19.858 1.00 7.98 6 C ATOM 1787 CB ALAD 4 27.122 33.625 20.025 1.00 6.69 6 C ATOM 1788 C ALA D 4 28.519 31.98118.712 1.00 8.86 6 C ATOM 1789 O ALA D 4 29.734 32.022 18.997 1.00 6.468 O ATOM 1790 N PHE D 5 28.015 31.833 17.488 1.00 6.65 7 N ATOM 1791 CAPHE D 5 28.883 31.817 16.321 1.00 6.21 6 C ATOM 1792 CB PHE D 5 28.69130.573 15.466 1.00 6.58 6 C ATOM 1793 CG PHE D 5 29.300 29.326 16.0451.00 6.64 6 C ATOM 1794 CD1 PHE D 5 28.596 28.550 16.929 1.00 8.03 6 CATOM 1795 CD2 PHE D 5 30.594 28.964 15.709 1.00 8.47 6 C ATOM 1796 CE1PHE D 5 29.137 27.383 17.485 1.00 8.72 6 C ATOM 1797 CE2 PHE D 5 31.15727.771 16.245 1.00 9.33 6 C ATOM 1798 CZ PHE D 5 30.417 27.024 17.1081.00 9.51 6 C ATOM 1799 C PHE D 5 28.529 33.046 15.478 1.00 8.92 6 CATOM 1800 O PHE D 5 27.347 33.369 15.226 1.00 7.43 8 O ATOM 1801 N ILE D6 29.571 33.778 15.043 1.00 6.33 7 N ATOM 1802 CA ILE D 6 29.321 35.02014.346 1.00 5.35 6 C ATOM 1803 CB ILE D 6 29.968 36.242 14.991 1.00 7.056 C ATOM 1804 CG2 ILE D 6 29.512 37.544 14.306 1.00 6.83 6 C ATOM 1805CG1 ILE D 6 29.682 36.312 16.498 1.00 9.71 6 C ATOM 1806 CD1 ILE D 630.583 37.312 17.230 1.00 10.38 6 C ATOM 1807 C ILE D 6 29.965 34.95212.940 1.00 7.08 6 C ATOM 1808 O ILE D 6 31.167 34.685 12.840 1.00 6.638 O ATOM 1809 N LEU D 7 29.158 35.223 11.940 1.00 6.66 7 N ATOM 1810 CALEU D 7 29.569 35.267 10.534 1.00 8.26 6 C ATOM 1811 CB LEU D 7 28.49034.694 9.625 1.00 9.39 6 C ATOM 1812 CG LEU D 7 28.278 33.182 9.719 1.0012.12 6 C ATOM 1813 CD1 LEU D 7 26.964 32.819 9.001 1.00 12.30 6 C ATOM1814 CD2 LEU D 7 29.452 32.442 9.113 1.00 12.62 6 C ATOM 1815 C LEU D 729.753 36.727 10.173 1.00 9.44 6 C ATOM 1816 O LEU D 7 28.969 37.61210.588 1.00 8.72 8 O ATOM 1817 N MET D 8 30.883 37.069 9.557 1.00 7.27 7N ATOM 1818 CA MET D 8 31.248 38.430 9.267 1.00 7.95 6 C ATOM 1819 CBMET D 8 32.497 38.770 10.154 1.00 9.16 6 C ATOM 1820 CG MET D 8 32.24038.575 11.637 1.00 12.23 6 C ATOM 1821 SD MET D 8 33.642 39.025 12.6681.00 9.99 16 S ATOM 1822 CE MET D 8 33.323 38.190 14.198 1.00 15.38 6 CATOM 1823 C MET D 8 31.695 38.640 7.819 1.00 9.51 6 C ATOM 1824 O MET D8 32.466 37.818 7.278 1.00 7.50 8 O ATOM 1825 N VAL D 9 31.387 39.8297.340 1.00 8.02 7 N ATOM 1826 CA VAL D 9 31.905 40.303 6.047 1.00 10.826 C ATOM 1827 CB VAL D 9 30.854 40.707 5.000 1.00 11.20 6 C ATOM 1828CG1 VAL D 9 31.500 41.169 3.666 1.00 11.05 6 C ATOM 1829 CG2 VAL D 929.940 39.525 4.650 1.00 10.58 6 C ATOM 1830 C VAL D 9 32.812 41.4826.401 1.00 10.74 6 C ATOM 1831 O VAL D 9 32.330 42.332 7.162 1.00 11.168 O ATOM 1832 N THR D 10 34.049 41.503 5.908 1.00 8.55 7 N ATOM 1833 CATHR D 10 34.950 42.605 6.235 1.00 9.63 6 C ATOM 1834 CB THR D 10 36.31642.145 6.788 1.00 7.41 6 C ATOM 1835 OG1 THR D 10 37.064 41.489 5.7311.00 7.90 8 O ATOM 1836 CG2 THR D 10 36.134 41.119 7.892 1.00 6.20 6 CATOM 1837 C THR D 10 35.214 43.436 4.976 1.00 10.07 6 C ATOM 1838 O THRD 10 34.887 42.902 3.935 1.00 5.93 8 O ATOM 1839 N ALA D 11 35.78144.632 5.043 1.00 8.15 7 N ATOM 1840 CA ALA D 11 36.193 45.341 3.8621.00 10.45 6 C ATOM 1841 CB ALA D 11 36.773 46.737 4.229 1.00 9.59 6 CATOM 1842 C ALA D 11 37.261 44.555 3.115 1.00 11.75 6 C ATOM 1843 O ALAD 11 38.008 43.789 3.744 1.00 11.01 8 O ATOM 1844 N ALA D 12 37.42844.805 1.813 1.00 11.13 7 N ATOM 1845 CA ALA D 12 38.439 44.106 1.0301.00 11.80 6 C ATOM 1846 CB ALA D 12 38.456 44.611 −0.421 1.00 11.09 6 CATOM 1847 C ALA D 12 39.838 44.330 1.596 1.00 10.46 6 C ATOM 1848 O ALAD 12 40.159 45.459 1.986 1.00 8.93 8 O ATOM 1849 N GLY D 13 40.63243.273 1.670 1.00 11.63 7 N ATOM 1850 CA GLY D 13 42.018 43.352 2.1131.00 9.40 6 C ATOM 1851 C GLY D 13 42.048 43.563 3.634 1.00 10.22 6 CATOM 1852 O GLY D 13 43.122 43.919 4.107 1.00 7.69 8 O ATOM 1853 N LYS D14 40.965 43.319 4.365 1.00 8.00 7 N ATOM 1854 CA LYS D 14 41.015 43.5495.807 1.00 10.05 6 C ATOM 1855 CB LYS D 14 39.991 44.631 6.218 1.0011.34 6 C ATOM 1856 CG LYS D 14 40.323 46.070 5.765 1.00 13.42 6 C ATOM1857 CD LYS D 14 41.685 46.502 6.343 1.00 15.94 6 C ATOM 1858 CE LYS D14 41.973 47.965 6.014 1.00 17.06 6 C ATOM 1859 NZ LYS D 14 43.25848.433 6.607 1.00 15.63 7 N ATOM 1860 C LYS D 14 40.739 42.306 6.6121.00 8.73 6 C ATOM 1861 O LYS D 14 40.729 42.391 7.849 1.00 8.72 8 OATOM 1862 N GLU D 15 40.392 41.175 5.995 1.00 8.24 7 N ATOM 1863 CA GLUD 15 40.019 40.018 6.817 1.00 11.30 6 C ATOM 1864 CB GLU D 15 39.55238.840 6.006 1.00 12.74 6 C ATOM 1865 CG GLU D 15 40.569 38.210 5.0631.00 13.46 6 C ATOM 1866 CD GLU D 15 39.892 37.220 4.102 1.00 15.23 6 CATOM 1867 OE1 GLU D 15 38.702 36.868 4.120 1.00 10.38 8 O ATOM 1868 OE2GLU D 15 40.658 36.700 3.250 1.00 17.69 8 O ATOM 1869 C GLU D 15 41.15239.617 7.758 1.00 11.05 6 C ATOM 1870 O GLU D 15 40.864 39.297 8.9071.00 11.86 8 O ATOM 1871 N ARG D 16 42.382 39.633 7.311 1.00 11.96 7 NATOM 1872 CA ARG D 16 43.472 39.262 8.208 1.00 14.89 6 C ATOM 1873 CBARG D 16 44.805 39.220 7.437 1.00 17.84 6 C ATOM 1874 CG ARG D 16 45.96639.007 8.388 1.00 25.50 6 C ATOM 1875 CD ARG D 16 47.263 38.621 7.6491.00 31.98 6 C ATOM 1876 NE ARG D 16 48.220 38.168 8.650 1.00 37.59 7 NATOM 1877 CZ ARG D 16 49.449 37.704 8.576 1.00 40.87 6 C ATOM 1878 NH1ARG D 16 50.073 37.573 7.404 1.00 43.35 7 N ATOM 1879 NH2 ARG D 1650.078 37.359 9.694 1.00 41.61 7 N ATOM 1880 C ARG D 16 43.580 40.2659.363 1.00 11.57 6 C ATOM 1881 O ARG D 16 43.846 39.856 10.471 1.00 7.548 O ATOM 1882 N GLU D 17 43.509 41.574 9.058 1.00 8.70 7 N ATOM 1883 CAGLU D 17 43.520 42.590 10.104 1.00 11.16 6 C ATOM 1884 CB GLU D 1743.356 43.975 9.464 1.00 10.95 6 C ATOM 1885 CG GLU D 17 43.321 45.16110.442 1.00 11.37 6 C ATOM 1886 CD GLU D 17 43.336 46.423 9.614 1.0014.08 6 C ATOM 1887 OE1 GLU D 17 44.230 46.626 8.769 1.00 11.49 8 O ATOM1888 OE2 GLU D 17 42.391 47.252 9.756 1.00 14.65 8 O ATOM 1889 C GLU D17 42.415 42.345 11.131 1.00 10.84 6 C ATOM 1890 O GLU D 17 42.61042.536 12.360 1.00 9.09 8 O ATOM 1891 N VAL D 18 41.222 41.917 10.6781.00 9.00 7 N ATOM 1892 CA VAL D 18 40.148 41.641 11.625 1.00 9.27 6 CATOM 1893 CB VAL D 18 38.804 41.488 10.876 1.00 9.98 6 C ATOM 1894 CG1VAL D 18 37.657 41.058 11.792 1.00 9.93 6 C ATOM 1895 CG2 VAL D 1838.445 42.837 10.240 1.00 8.46 6 C ATOM 1896 C VAL D 18 40.467 40.42012.475 1.00 8.32 6 C ATOM 1897 O VAL D 18 40.313 40.366 13.706 1.00 6.468 O ATOM 1898 N MET D 19 40.966 39.375 11.776 1.00 5.78 7 N ATOM 1899 CAMET D 19 41.370 38.173 12.470 1.00 7.84 6 C ATOM 1900 CB MET D 19 42.01237.108 11.544 1.00 7.18 6 C ATOM 1901 CG MET D 19 40.955 36.423 10.6741.00 10.79 6 C ATOM 1902 SD MET D 19 40.066 35.123 11.568 1.00 9.43 16 SATOM 1903 CE MET D 19 41.320 33.849 11.585 1.00 13.35 6 C ATOM 1904 CMET D 19 42.380 38.513 13.580 1.00 8.79 6 C ATOM 1905 O MET D 19 42.39837.821 14.587 1.00 6.86 8 O ATOM 1906 N GLU D 20 43.327 39.417 13.2891.00 8.74 7 N ATOM 1907 CA GLU D 20 44.322 39.799 14.331 1.00 12.83 6 CATOM 1908 CB GLU D 20 45.435 40.642 13.699 1.00 11.30 6 C ATOM 1909 CGGLU D 20 46.357 39.657 12.930 1.00 13.63 6 C ATOM 1910 CD GLU D 2047.519 40.364 12.253 1.00 17.21 6 C ATOM 1911 OE1 GLU D 20 47.597 41.62012.258 1.00 17.52 8 O ATOM 1912 OE2 GLU D 20 48.352 39.656 11.622 1.0017.62 8 O ATOM 1913 C GLU D 20 43.639 40.472 15.520 1.00 12.42 6 C ATOM1914 O GLU D 20 43.980 40.207 16.673 1.00 13.73 8 O ATOM 1915 N LYS D 2142.617 41.275 15.271 1.00 10.66 7 N ATOM 1916 CA LYS D 21 41.854 41.86716.365 1.00 11.11 6 C ATOM 1917 CB LYS D 21 40.878 42.958 15.892 1.0010.31 6 C ATOM 1918 CG LYS D 21 41.568 44.254 15.563 1.00 11.75 6 C ATOM1919 CD LYS D 21 40.551 45.276 15.062 1.00 12.69 6 C ATOM 1920 CE LYS D21 41.190 46.632 14.741 1.00 14.75 6 C ATOM 1921 NZ LYS D 21 40.10747.556 14.250 1.00 12.59 7 N ATOM 1922 C LYS D 21 41.115 40.792 17.1361.00 8.78 6 C ATOM 1923 O LYS D 21 41.229 40.694 18.372 1.00 10.75 8 OATOM 1924 N LEU D 22 40.465 39.846 16.472 1.00 10.46 7 N ATOM 1925 CALEU D 22 39.667 38.802 17.134 1.00 8.87 6 C ATOM 1926 CB LEU D 22 38.92037.980 16.061 1.00 8.01 6 C ATOM 1927 CG LEU D 22 37.883 38.808 15.2711.00 10.13 6 C ATOM 1928 CD1 LEU D 22 37.464 38.033 14.021 1.00 9.62 6 CATOM 1929 CD2 LEU D 22 36.664 39.137 16.170 1.00 8.70 6 C ATOM 1930 CLEU D 22 40.536 37.837 17.936 1.00 10.00 6 C ATOM 1931 O LEU D 22 40.17137.498 19.077 1.00 7.47 8 O ATOM 1932 N LEU D 23 41.707 37.413 17.3941.00 7.02 7 N ATOM 1933 CA LEU D 23 42.521 36.497 18.203 1.00 10.23 6 CATOM 1934 CB LEU D 23 43.459 35.688 17.309 1.00 11.12 6 C ATOM 1935 CGLEU D 23 42.767 34.912 16.180 1.00 13.04 6 C ATOM 1936 CD1 LEU D 2343.728 34.528 15.057 1.00 11.71 6 C ATOM 1937 CD2 LEU D 23 42.107 33.66616.753 1.00 12.37 6 C ATOM 1938 C LEU D 23 43.234 37.115 19.399 1.0010.47 6 C ATOM 1939 O LEU D 23 43.943 36.413 20.157 1.00 10.67 8 O ATOM1940 N ALA D 24 43.032 38.376 19.741 1.00 8.71 7 N ATOM 1941 CA ALA D 2443.504 39.013 20.928 1.00 10.88 6 C ATOM 1942 CB ALA D 24 44.146 40.39520.663 1.00 10.61 6 C ATOM 1943 C ALA D 24 42.298 39.250 21.861 1.0012.75 6 C ATOM 1944 O ALA D 24 42.581 39.689 22.961 1.00 11.80 8 O ATOM1945 N MET D 25 41.070 38.842 21.482 1.00 10.78 7 N ATOM 1946 CA MET D25 39.984 39.163 22.443 1.00 12.47 6 C ATOM 1947 CB MET D 25 38.72439.513 21.657 1.00 11.87 6 C ATOM 1948 CG MET D 25 38.837 40.708 20.7241.00 9.43 6 C ATOM 1949 SD MET D 25 37.279 40.909 19.751 1.00 7.93 16 SATOM 1950 CE MET D 25 37.656 42.472 18.986 1.00 8.17 6 C ATOM 1951 C METD 25 39.756 37.981 23.364 1.00 10.74 6 C ATOM 1952 O MET D 25 39.68936.838 22.907 1.00 10.38 8 O ATOM 1953 N PRO D 26 39.638 38.173 24.6621.00 10.43 7 N ATOM 1954 CD PRO D 26 39.570 39.495 25.327 1.00 11.37 6 CATOM 1955 CA PRO D 26 39.297 37.068 25.552 1.00 10.14 6 C ATOM 1956 CBPRO D 26 38.977 37.760 26.908 1.00 11.47 6 C ATOM 1957 CG PRO D 2639.571 39.129 26.804 1.00 13.17 6 C ATOM 1958 C PRO D 26 38.094 36.22425.147 1.00 8.78 6 C ATOM 1959 O PRO D 26 38.113 34.961 25.329 1.00 7.448 O ATOM 1960 N GLU D 27 37.067 36.856 24.599 1.00 7.06 7 N ATOM 1961 CAGLU D 27 35.809 36.171 24.302 1.00 9.61 6 C ATOM 1962 CB GLU D 27 34.69337.232 24.074 1.00 10.96 6 C ATOM 1963 CG GLU D 27 34.496 38.142 25.2901.00 13.96 6 C ATOM 1964 CD GLU D 27 35.375 39.387 25.273 1.00 15.97 6 CATOM 1965 OE1 GLU D 27 36.379 39.456 24.532 1.00 13.92 8 O ATOM 1966 OE2GLU D 27 34.937 40.411 25.876 1.00 16.96 8 O ATOM 1967 C GLU D 27 35.81835.290 23.070 1.00 8.19 6 C ATOM 1968 O GLU D 27 35.104 34.293 23.0341.00 6.20 8 O ATOM 1969 N VAL D 28 36.862 35.512 22.223 1.00 8.44 7 NATOM 1970 CA VAL D 28 36.933 34.688 20.998 1.00 7.24 6 C ATOM 1971 CBVAL D 28 37.735 35.371 19.882 1.00 3.76 6 C ATOM 1972 CG1 VAL D 2838.115 34.476 18.758 1.00 3.75 6 C ATOM 1973 CG2 VAL D 28 36.991 36.64819.370 1.00 3.95 6 C ATOM 1974 C VAL D 28 37.547 33.354 21.308 1.00 9.276 C ATOM 1975 O VAL D 28 38.709 33.330 21.734 1.00 9.19 8 O ATOM 1976 NLYS D 29 36.868 32.228 21.065 1.00 9.77 7 N ATOM 1977 CA LYS D 29 37.43930.911 21.290 1.00 10.82 6 C ATOM 1978 CB LYS D 29 36.354 29.989 21.8601.00 12.93 6 C ATOM 1979 CG LYS D 29 35.708 30.606 23.097 1.00 15.22 6 CATOM 1980 CD LYS D 29 36.573 30.622 24.356 1.00 16.50 6 C ATOM 1981 CELYS D 29 36.919 32.063 24.674 1.00 18.62 6 C ATOM 1982 NZ LYS D 2937.533 32.336 26.009 1.00 20.84 7 N ATOM 1983 C LYS D 29 38.023 30.24220.062 1.00 11.20 6 C ATOM 1984 O LYS D 29 38.928 29.399 20.213 1.0010.74 8 O ATOM 1985 N GLU D 30 37.527 30.591 18.876 1.00 10.26 7 N ATOM1986 CA GLU D 30 38.068 30.038 17.625 1.00 10.81 6 C ATOM 1987 CB GLU D30 37.655 28.615 17.277 1.00 13.90 6 C ATOM 1988 CG GLU D 30 36.19828.375 16.965 1.00 18.66 6 C ATOM 1989 CD GLU D 30 35.861 26.876 16.7891.00 20.56 6 C ATOM 1990 OE1 GLU D 30 36.678 26.028 16.448 1.00 17.38 8O ATOM 1991 OE2 GLU D 30 34.684 26.508 16.956 1.00 21.70 8 O ATOM 1992 CGLU D 30 37.675 31.011 16.512 1.00 10.06 6 C ATOM 1993 O GLU D 30 36.60931.646 16.617 1.00 7.37 8 O ATOM 1994 N ALA D 31 38.502 31.177 15.5071.00 8.02 7 N ATOM 1995 CA ALA D 31 38.200 32.119 14.421 1.00 7.12 6 CATOM 1996 CB ALA D 31 38.807 33.489 14.683 1.00 8.42 6 C ATOM 1997 C ALAD 31 38.745 31.565 13.117 1.00 8.88 6 C ATOM 1998 O ALA D 31 39.88431.020 13.144 1.00 8.65 8 O ATOM 1999 N TYR D 32 37.999 31.613 12.0011.00 5.43 7 N ATOM 2000 CA TYR D 32 38.546 31.045 10.796 1.00 6.36 6 CATOM 2001 CB TYR D 32 37.990 29.674 10.391 1.00 8.15 6 C ATOM 2002 CGTYR D 32 38.426 28.515 11.256 1.00 10.64 6 C ATOM 2003 CD1 TYR D 3239.545 27.765 10.930 1.00 13.77 6 C ATOM 2004 CE1 TYR D 32 39.920 26.68211.711 1.00 15.51 6 C ATOM 2005 CD2 TYR D 32 37.754 28.227 12.419 1.0011.24 6 C ATOM 2006 CE2 TYR D 32 38.115 27.173 13.217 1.00 12.74 6 CATOM 2007 CZ TYR D 32 39.201 26.419 12.861 1.00 14.10 6 C ATOM 2008 OHTYR D 32 39.533 25.377 13.684 1.00 16.30 8 O ATOM 2009 C TYR D 32 38.15431.873 9.560 1.00 7.98 6 C ATOM 2010 O TYR D 32 37.040 32.416 9.586 1.007.94 8 O ATOM 2011 N VAL D 33 39.074 31.877 8.611 1.00 6.38 7 N ATOM2012 CA VAL D 33 38.758 32.536 7.348 1.00 7.52 6 C ATOM 2013 CB VAL D 3339.981 32.990 6.536 1.00 8.07 6 C ATOM 2014 CG1 VAL D 33 39.567 33.5175.162 1.00 6.28 6 C ATOM 2015 CG2 VAL D 33 40.723 34.076 7.330 1.00 7.686 C ATOM 2016 C VAL D 33 37.968 31.475 6.595 1.00 7.23 6 C ATOM 2017 OVAL D 33 38.398 30.299 6.681 1.00 10.00 8 O ATOM 2018 N VAL D 34 36.78431.773 6.055 1.00 6.32 7 N ATOM 2019 CA VAL D 34 36.024 30.715 5.3721.00 6.20 6 C ATOM 2020 CB VAL D 34 34.708 30.380 6.088 1.00 8.45 6 CATOM 2021 CG1 VAL D 34 34.870 29.568 7.387 1.00 8.52 6 C ATOM 2022 CG2VAL D 34 33.920 31.673 6.419 1.00 8.05 6 C ATOM 2023 C VAL D 34 35.68731.195 3.959 1.00 8.31 6 C ATOM 2024 O VAL D 34 35.649 32.430 3.697 1.008.44 8 O ATOM 2025 N TYR D 35 35.506 30.287 3.043 1.00 7.60 7 N ATOM2026 CA TYR D 35 35.035 30.540 1.693 1.00 10.70 6 C ATOM 2027 CB TYR D35 34.992 29.145 0.966 1.00 13.94 6 C ATOM 2028 CG TYR D 35 36.28728.648 0.406 1.00 19.41 6 C ATOM 2029 CD1 TYR D 35 36.500 28.632 −0.9761.00 21.68 6 C ATOM 2030 CE1 TYR D 35 37.708 28.179 −1.499 1.00 23.69 6C ATOM 2031 CD2 TYR D 35 37.334 28.196 1.232 1.00 21.93 6 C ATOM 2032CE2 TYR D 35 38.533 27.739 0.695 1.00 21.21 6 C ATOM 2033 CZ TYR D 3538.714 27.728 −0.672 1.00 23.19 6 C ATOM 2034 OH TYR D 35 39.907 27.295−1.250 1.00 23.55 8 O ATOM 2035 C TYR D 35 33.570 31.004 1.702 1.00 9.186 C ATOM 2036 O TYR D 35 32.887 30.788 2.719 1.00 7.05 8 O ATOM 2037 NGLY D 36 33.114 31.550 0.583 1.00 9.23 7 N ATOM 2038 CA GLY D 36 31.70331.882 0.420 1.00 8.59 6 C ATOM 2039 C GLY D 36 31.335 33.330 0.669 1.0010.03 6 C ATOM 2040 O GLY D 36 32.197 34.227 0.709 1.00 8.03 8 O ATOM2041 N GLU D 37 30.038 33.530 0.994 1.00 9.60 7 N ATOM 2042 CA GLU D 3729.553 34.874 1.190 1.00 13.84 6 C ATOM 2043 CB GLU D 37 28.042 34.8881.222 1.00 20.20 6 C ATOM 2044 CG GLU D 37 27.302 34.244 2.338 1.0026.34 6 C ATOM 2045 CD GLU D 37 25.826 34.649 2.298 1.00 29.65 6 C ATOM2046 OE1 GLU D 37 24.966 33.760 2.335 1.00 33.05 8 O ATOM 2047 OE2 GLU D37 25.505 35.844 2.279 1.00 32.09 8 O ATOM 2048 C GLU D 37 30.156 35.5892.393 1.00 13.26 6 C ATOM 2049 O GLU D 37 30.257 36.818 2.316 1.00 11.838 O ATOM 2050 N TYR D 38 30.463 34.895 3.458 1.00 11.24 7 N ATOM 2051 CATYR D 38 31.113 35.525 4.609 1.00 13.22 6 C ATOM 2052 CB TYR D 38 30.60234.920 5.924 1.00 14.41 6 C ATOM 2053 CG TYR D 38 29.125 35.241 6.0861.00 17.44 6 C ATOM 2054 CD1 TYR D 38 28.751 36.473 6.607 1.00 19.66 6 CATOM 2055 CE1 TYR D 38 27.404 36.793 6.741 1.00 22.87 6 C ATOM 2056 CD2TYR D 38 28.164 34.332 5.690 1.00 19.08 6 C ATOM 2057 CE2 TYR D 3826.806 34.627 5.817 1.00 21.26 6 C ATOM 2058 CZ TYR D 38 26.460 35.8516.360 1.00 23.15 6 C ATOM 2059 OH TYR D 38 25.146 36.171 6.515 1.0025.72 8 O ATOM 2060 C TYR D 38 32.632 35.321 4.547 1.00 12.41 6 C ATOM2061 O TYR D 38 33.089 34.307 4.013 1.00 8.37 8 O ATOM 2062 N ASP D 3933.400 36.289 5.061 1.00 11.84 7 N ATOM 2063 CA ASP D 39 34.846 36.1275.064 1.00 10.07 6 C ATOM 2064 CB ASP D 39 35.517 37.526 5.140 1.0012.25 6 C ATOM 2065 CG ASP D 39 35.115 38.291 3.866 1.00 12.99 6 C ATOM2066 OD1 ASP D 39 35.289 37.666 2.802 1.00 11.90 8 O ATOM 2067 OD2 ASP D39 34.602 39.408 3.976 1.00 12.15 8 O ATOM 2068 C ASP D 39 35.285 35.2996.253 1.00 8.98 6 C ATOM 2069 O ASP D 39 36.213 34.516 6.139 1.00 9.58 8O ATOM 2070 N LEU D 40 34.658 35.524 7.416 1.00 5.50 7 N ATOM 2071 CALEU D 40 35.088 34.829 8.633 1.00 7.68 6 C ATOM 2072 CB LEU D 40 35.71535.942 9.546 1.00 8.52 6 C ATOM 2073 CG LEU D 40 36.837 36.793 9.0091.00 14.09 6 C ATOM 2074 CD1 LEU D 40 37.168 37.996 9.919 1.00 13.28 6 CATOM 2075 CD2 LEU D 40 38.110 35.938 8.801 1.00 14.05 6 C ATOM 2076 CLEU D 40 33.952 34.236 9.461 1.00 7.29 6 C ATOM 2077 O LEU D 40 32.83434.770 9.431 1.00 7.50 8 O ATOM 2078 N ILE D 41 34.225 33.291 10.3081.00 6.52 7 N ATOM 2079 CA ILE D 41 33.347 32.659 11.251 1.00 8.65 6 CATOM 2080 CB ILE D 41 32.757 31.294 10.866 1.00 7.09 6 C ATOM 2081 CG2ILE D 41 33.763 30.136 10.738 1.00 7.76 6 C ATOM 2082 CG1 ILE D 4131.714 30.864 11.933 1.00 7.44 6 C ATOM 2083 CD1 ILE D 41 30.882 29.69211.430 1.00 8.47 6 C ATOM 2084 C ILE D 41 34.095 32.701 12.586 1.0010.10 6 C ATOM 2085 O ILE D 41 35.293 32.408 12.685 1.00 9.03 8 O ATOM2086 N VAL D 42 33.391 33.059 13.669 1.00 10.09 7 N ATOM 2087 CA VAL D42 34.073 33.170 14.978 1.00 10.73 6 C ATOM 2088 CB VAL D 42 34.40434.614 15.388 1.00 13.00 6 C ATOM 2089 CG1 VAL D 42 33.245 35.362 16.0631.00 14.99 6 C ATOM 2090 CG2 VAL D 42 35.471 34.830 16.425 1.00 12.34 6C ATOM 2091 C VAL D 42 33.159 32.540 16.008 1.00 9.22 6 C ATOM 2092 OVAL D 42 31.939 32.782 15.940 1.00 9.43 8 O ATOM 2093 N LYS D 43 33.73931.804 16.934 1.00 6.57 7 N ATOM 2094 CA LYS D 43 32.976 31.281 18.0491.00 7.49 6 C ATOM 2095 CB LYS D 43 33.242 29.814 18.298 1.00 8.66 6 CATOM 2096 CG LYS D 43 32.509 29.240 19.502 1.00 10.98 6 C ATOM 2097 CDLYS D 43 32.987 27.756 19.527 1.00 12.07 6 C ATOM 2098 CE LYS D 4332.765 27.099 20.873 1.00 14.03 6 C ATOM 2099 NZ LYS D 43 33.327 25.69520.768 1.00 12.83 7 N ATOM 2100 C LYS D 43 33.370 32.072 19.277 1.007.78 6 C ATOM 2101 O LYS D 43 34.586 32.291 19.497 1.00 8.73 8 O ATOM2102 N VAL D 44 32.387 32.621 19.972 1.00 8.73 7 N ATOM 2103 CA VAL D 4432.629 33.468 21.129 1.00 7.44 6 C ATOM 2104 CB VAL D 44 32.310 34.94120.905 1.00 8.98 6 C ATOM 2105 CG1 VAL D 44 33.072 35.522 19.705 1.007.87 6 C ATOM 2106 CG2 VAL D 44 30.806 35.154 20.649 1.00 8.94 6 C ATOM2107 C VAL D 44 31.798 32.956 22.304 1.00 9.01 6 C ATOM 2108 O VAL D 4430.837 32.209 22.117 1.00 9.60 8 O ATOM 2109 N GLU D 45 32.254 33.19723.525 1.00 9.31 7 N ATOM 2110 CA GLU D 45 31.678 32.770 24.753 1.0012.52 6 C ATOM 2111 CB GLU D 45 32.392 31.581 25.436 1.00 14.38 6 C ATOM2112 CG GLU D 45 32.127 30.265 24.685 1.00 18.54 6 C ATOM 2113 CD GLU D45 32.979 29.114 25.184 1.00 22.44 6 C ATOM 2114 OE1 GLU D 45 33.02528.070 24.514 1.00 23.21 8 O ATOM 2115 OE2 GLU D 45 33.613 29.221 26.2631.00 23.95 8 O ATOM 2116 C GLU D 45 31.715 33.963 25.718 1.00 13.18 6 CATOM 2117 O GLU D 45 32.691 34.687 25.785 1.00 10.66 8 O ATOM 2118 N THRD 46 30.602 34.241 26.380 1.00 13.36 7 N ATOM 2119 CA THR D 46 30.52735.298 27.381 1.00 13.78 6 C ATOM 2120 CB THR D 46 29.805 36.570 26.9151.00 15.75 6 C ATOM 2121 OG1 THR D 46 28.450 36.171 26.648 1.00 14.48 8O ATOM 2122 CG2 THR D 46 30.488 37.200 25.710 1.00 15.69 6 C ATOM 2123 CTHR D 46 29.791 34.679 28.574 1.00 14.47 6 C ATOM 2124 O THR D 46 29.06933.663 28.418 1.00 13.33 8 O ATOM 2125 N ASP D 47 29.938 35.295 29.7351.00 13.95 7 N ATOM 2126 CA ASP D 47 29.336 34.725 30.929 1.00 16.99 6 CATOM 2127 CB ASP D 47 29.734 35.409 32.247 1.00 22.34 6 C ATOM 2128 CGASP D 47 31.173 35.190 32.639 1.00 27.62 6 C ATOM 2129 OD1 ASP D 4731.825 34.359 31.947 1.00 31.21 8 O ATOM 2130 OD2 ASP D 47 31.713 35.78233.605 1.00 30.67 8 O ATOM 2131 C ASP D 47 27.819 34.864 30.828 1.0014.12 6 C ATOM 2132 O ASP D 47 27.153 33.940 31.227 1.00 15.12 8 O ATOM2133 N THR D 48 27.297 35.969 30.327 1.00 11.97 7 N ATOM 2134 CA THR D48 25.875 36.189 30.244 1.00 13.15 6 C ATOM 2135 CB THR D 48 25.37337.236 31.240 1.00 13.15 6 C ATOM 2136 OG1 THR D 48 26.017 38.511 30.9731.00 15.41 8 O ATOM 2137 CG2 THR D 48 25.612 36.804 32.692 1.00 14.04 6C ATOM 2138 C THR D 48 25.446 36.742 28.877 1.00 14.57 6 C ATOM 2139 OTHR D 48 26.291 37.155 28.065 1.00 12.38 8 O ATOM 2140 N LEU D 49 24.12536.733 28.685 1.00 11.23 7 N ATOM 2141 CA LEU D 49 23.583 37.284 27.4371.00 13.73 6 C ATOM 2142 CB LEU D 49 22.065 37.080 27.339 1.00 12.51 6 CATOM 2143 CG LEU D 49 21.364 37.558 26.069 1.00 14.18 6 C ATOM 2144 CD1LEU D 49 22.014 37.002 24.817 1.00 11.03 6 C ATOM 2145 CD2 LEU D 4919.881 37.088 26.143 1.00 13.29 6 C ATOM 2146 C LEU D 49 23.855 38.77827.338 1.00 13.40 6 C ATOM 2147 O LEU D 49 24.102 39.374 26.296 1.0013.30 8 O ATOM 2148 N LYS D 50 23.652 39.471 28.462 1.00 13.92 7 N ATOM2149 CA LYS D 50 23.916 40.892 28.544 1.00 16.34 6 C ATOM 2150 CB LYS D50 23.691 41.327 30.019 1.00 20.47 6 C ATOM 2151 CG LYS D 50 23.30242.793 30.072 1.00 26.88 6 C ATOM 2152 CD LYS D 50 24.075 43.538 31.1511.00 31.08 6 C ATOM 2153 CE LYS D 50 23.804 45.046 31.108 1.00 32.48 6 CATOM 2154 NZ LYS D 50 23.836 45.593 32.509 1.00 34.98 7 N ATOM 2155 CLYS D 50 25.348 41.230 28.148 1.00 13.33 6 C ATOM 2156 O LYS D 50 25.61442.240 27.468 1.00 13.39 8 O ATOM 2157 N ASP D 51 26.299 40.411 28.5631.00 12.48 7 N ATOM 2158 CA ASP D 51 27.692 40.626 28.146 1.00 15.10 6 CATOM 2159 CB ASP D 51 28.663 39.759 28.947 1.00 16.35 6 C ATOM 2160 CGASP D 51 28.728 40.315 30.377 1.00 18.78 6 C ATOM 2161 OD1 ASP D 5128.465 41.527 30.553 1.00 19.84 8 O ATOM 2162 OD2 ASP D 51 29.036 39.46831.217 1.00 19.28 8 O ATOM 2163 C ASP D 51 27.868 40.329 26.659 1.0012.69 6 C ATOM 2164 O ASP D 51 28.701 40.979 26.028 1.00 14.63 8 O ATOM2165 N LEU D 52 27.187 39.306 26.139 1.00 11.08 7 N ATOM 2166 CA LEU D52 27.246 39.107 24.685 1.00 10.83 6 C ATOM 2167 CB LEU D 52 26.45637.864 24.267 1.00 10.10 6 C ATOM 2168 CG LEU D 52 26.605 37.462 22.7931.00 9.50 6 C ATOM 2169 CD1 LEU D 52 28.001 36.865 22.523 1.00 9.81 6 CATOM 2170 CD2 LEU D 52 25.529 36.464 22.405 1.00 7.72 6 C ATOM 2171 CLEU D 52 26.704 40.331 23.976 1.00 12.03 6 C ATOM 2172 O LEU D 52 27.28940.837 22.985 1.00 10.16 8 O ATOM 2173 N ASP D 53 25.546 40.859 24.4791.00 11.64 7 N ATOM 2174 CA ASP D 53 24.983 42.043 23.860 1.00 12.95 6 CATOM 2175 CB ASP D 53 23.750 42.582 24.595 1.00 14.34 6 C ATOM 2176 CGASP D 53 22.563 41.630 24.609 1.00 15.63 6 C ATOM 2177 OD1 ASP D 5322.449 40.684 23.791 1.00 15.62 8 O ATOM 2178 OD2 ASP D 53 21.691 41.82425.492 1.00 16.34 8 O ATOM 2179 C ASP D 53 26.040 43.147 23.688 1.0015.69 6 C ATOM 2180 O ASP D 53 26.150 43.779 22.627 1.00 13.41 8 O ATOM2181 N GLN D 54 26.714 43.454 24.782 1.00 15.39 7 N ATOM 2182 CA GLN D54 27.750 44.490 24.826 1.00 19.04 6 C ATOM 2183 CB GLN D 54 28.19644.595 26.286 1.00 21.11 6 C ATOM 2184 CG GLN D 54 28.911 45.886 26.6191.00 30.34 6 C ATOM 2185 CD GLN D 54 29.355 45.897 28.070 1.00 35.43 6 CATOM 2186 OE1 GLN D 54 29.038 45.001 28.873 1.00 38.74 8 O ATOM 2187 NE2GLN D 54 30.131 46.936 28.390 1.00 37.61 7 N ATOM 2188 C GLN D 54 28.94344.195 23.929 1.00 13.99 6 C ATOM 2189 O GLN D 54 29.388 45.028 23.1621.00 12.65 8 O ATOM 2190 N PHE D 55 29.447 42.978 23.978 1.00 15.43 7 NATOM 2191 CA PHE D 55 30.586 42.554 23.134 1.00 13.50 6 C ATOM 2192 CBPHE D 55 30.897 41.093 23.483 1.00 12.42 6 C ATOM 2193 CG PHE D 5532.002 40.549 22.602 1.00 11.54 6 C ATOM 2194 CD1 PHE D 55 33.319 41.01522.767 1.00 9.95 6 C ATOM 2195 CD2 PHE D 55 31.713 39.571 21.668 1.009.64 6 C ATOM 2196 CE1 PHE D 55 34.292 40.453 21.936 1.00 12.19 6 C ATOM2197 CE2 PHE D 55 32.705 39.010 20.874 1.00 10.13 6 C ATOM 2198 CZ PHE D55 34.002 39.465 21.026 1.00 9.99 6 C ATOM 2199 C PHE D 55 30.255 42.71421.653 1.00 13.58 6 C ATOM 2200 O PHE D 55 30.888 43.510 20.915 1.0010.92 8 O ATOM 2201 N ILE D 56 29.107 42.119 21.255 1.00 10.76 7 N ATOM2202 CA ILE D 56 28.785 42.189 19.830 1.00 11.96 6 C ATOM 2203 CB ILE D56 27.579 41.278 19.498 1.00 13.95 6 C ATOM 2204 CG2 ILE D 56 27.09641.404 18.056 1.00 12.24 6 C ATOM 2205 CG1 ILE D 56 28.011 39.898 19.9471.00 14.49 6 C ATOM 2206 CD1 ILE D 56 28.161 38.777 19.011 1.00 18.97 6C ATOM 2207 C ILE D 56 28.491 43.582 19.349 1.00 13.51 6 C ATOM 2208 OILE D 56 28.926 43.983 18.256 1.00 13.02 8 O ATOM 2209 N THR D 57 27.71044.341 20.110 1.00 15.60 7 N ATOM 2210 CA THR D 57 27.326 45.675 19.6211.00 17.80 6 C ATOM 2211 CB THR D 57 26.048 46.210 20.297 1.00 19.23 6 CATOM 2212 OG1 THR D 57 26.180 46.125 21.711 1.00 22.03 8 O ATOM 2213 CG2THR D 57 24.810 45.352 20.028 1.00 20.73 6 C ATOM 2214 C THR D 57 28.51846.636 19.744 1.00 20.35 6 C ATOM 2215 O THR D 57 28.824 47.295 18.7291.00 22.00 8 O ATOM 2216 N GLU D 58 29.389 46.530 20.744 1.00 20.17 7 NATOM 2217 CA GLU D 58 30.510 47.426 20.876 1.00 23.72 6 C ATOM 2218 CBGLU D 58 30.773 47.819 22.355 1.00 27.15 6 C ATOM 2219 CG GLU D 5829.557 48.536 22.967 1.00 30.14 6 C ATOM 2220 CD GLU D 58 29.380 49.91922.337 1.00 33.67 6 C ATOM 2221 OE1 GLU D 58 30.396 50.630 22.041 1.0035.18 8 O ATOM 2222 OE2 GLU D 58 28.204 50.285 22.109 1.00 34.26 8 OATOM 2223 C GLU D 58 31.818 46.930 20.286 1.00 22.92 6 C ATOM 2224 O GLUD 58 32.507 47.785 19.723 1.00 22.33 8 O ATOM 2225 N LYS D 59 32.20445.660 20.404 1.00 20.11 7 N ATOM 2226 CA LYS D 59 33.473 45.223 19.8731.00 20.10 6 C ATOM 2227 CB LYS D 59 34.111 44.113 20.719 1.00 21.48 6 CATOM 2228 CG LYS D 59 34.207 44.513 22.199 1.00 27.96 6 C ATOM 2229 CDLYS D 59 35.424 45.373 22.500 1.00 30.31 6 C ATOM 2230 CE LYS D 5935.427 45.865 23.946 1.00 32.73 6 C ATOM 2231 NZ LYS D 59 35.737 47.34724.028 1.00 34.31 7 N ATOM 2232 C LYS D 59 33.396 44.694 18.438 1.0019.09 6 C ATOM 2233 O LYS D 59 34.445 44.621 17.756 1.00 19.41 8 O ATOM2234 N ILE D 60 32.263 44.090 18.091 1.00 13.82 7 N ATOM 2235 CA ILE D60 32.200 43.489 16.762 1.00 12.95 6 C ATOM 2236 CB ILE D 60 31.55642.118 16.849 1.00 12.02 6 C ATOM 2237 CG2 ILE D 60 31.412 41.544 15.4291.00 11.69 6 C ATOM 2238 CG1 ILE D 60 32.335 41.135 17.750 1.00 12.06 6C ATOM 2239 CD1 ILE D 60 33.810 41.039 17.546 1.00 12.30 6 C ATOM 2240 CILE D 60 31.545 44.412 15.729 1.00 14.62 6 C ATOM 2241 O ILE D 60 32.12644.822 14.727 1.00 10.09 8 O ATOM 2242 N ARG D 61 30.278 44.774 15.9691.00 14.65 7 N ATOM 2243 CA ARG D 61 29.523 45.610 15.045 1.00 16.07 6 CATOM 2244 CB ARG D 61 28.038 45.579 15.500 1.00 16.92 6 C ATOM 2245 CGARG D 61 27.363 44.212 15.262 1.00 17.47 6 C ATOM 2246 CD ARG D 6125.893 44.275 15.640 1.00 22.16 6 C ATOM 2247 NE ARG D 61 25.086 43.06315.705 1.00 20.80 7 N ATOM 2248 CZ ARG D 61 23.799 42.867 15.496 1.0020.73 6 C ATOM 2249 NH1 ARG D 61 22.940 43.817 15.183 1.00 21.08 7 NATOM 2250 NH2 ARG D 61 23.194 41.692 15.674 1.00 19.70 7 N ATOM 2251 CARG D 61 30.081 47.007 14.834 1.00 15.30 6 C ATOM 2252 O ARG D 61 29.91947.548 13.729 1.00 12.83 8 O ATOM 2253 N LYS D 62 30.862 47.581 15.7261.00 13.69 7 N ATOM 2254 CA LYS D 62 31.537 48.845 15.612 1.00 16.28 6 CATOM 2255 CB LYS D 62 31.355 49.671 16.910 1.00 19.57 6 C ATOM 2256 CGLYS D 62 29.965 50.343 16.732 1.00 21.09 6 C ATOM 2257 CD LYS D 6229.347 50.574 18.073 1.00 24.81 6 C ATOM 2258 CE LYS D 62 28.160 51.53917.965 1.00 26.49 6 C ATOM 2259 NZ LYS D 62 27.744 51.698 19.399 1.0032.17 7 N ATOM 2260 C LYS D 62 32.991 48.777 15.185 1.00 15.48 6 C ATOM2261 O LYS D 62 33.619 49.794 14.910 1.00 13.01 8 O ATOM 2262 N MET D 6333.483 47.575 14.930 1.00 13.64 7 N ATOM 2263 CA MET D 63 34.840 47.46914.347 1.00 14.05 6 C ATOM 2264 CB MET D 63 35.250 46.002 14.371 1.0014.96 6 C ATOM 2265 CG MET D 63 36.702 45.765 13.995 1.00 17.59 6 C ATOM2266 SD MET D 63 37.077 44.017 13.778 1.00 16.00 16 S ATOM 2267 CE MET D63 36.816 43.394 15.447 1.00 16.24 6 C ATOM 2268 C MET D 63 34.79848.084 12.950 1.00 12.08 6 C ATOM 2269 O MET D 63 33.998 47.740 12.0791.00 8.64 8 O ATOM 2270 N PRO D 64 35.692 49.011 12.624 1.00 12.28 7 NATOM 2271 CD PRO D 64 36.723 49.571 13.578 1.00 13.06 6 C ATOM 2272 CAPRO D 64 35.610 49.760 11.398 1.00 11.60 6 C ATOM 2273 CB PRO D 6436.749 50.767 11.450 1.00 14.09 6 C ATOM 2274 CG PRO D 64 37.175 50.83812.874 1.00 14.47 6 C ATOM 2275 C PRO D 64 35.697 48.907 10.137 1.0011.66 6 C ATOM 2276 O PRO D 64 35.144 49.337 9.117 1.00 9.20 8 O ATOM2277 N GLU D 65 36.518 47.855 10.133 1.00 7.96 7 N ATOM 2278 CA GLU D 6536.670 46.978 8.990 1.00 10.89 6 C ATOM 2279 CB GLU D 65 37.858 46.0249.221 1.00 13.55 6 C ATOM 2280 CG GLU D 65 39.187 46.824 9.538 1.0013.78 6 C ATOM 2281 CD GLU D 65 39.461 46.983 11.033 1.00 16.55 6 C ATOM2282 OE1 GLU D 65 38.484 47.049 11.844 1.00 12.89 8 O ATOM 2283 OE2 GLUD 65 40.649 47.099 11.496 1.00 15.86 8 O ATOM 2284 C GLU D 65 35.40646.138 8.682 1.00 11.19 6 C ATOM 2285 O GLU D 65 35.350 45.423 7.6801.00 7.50 8 O ATOM 2286 N ILE D 66 34.519 45.952 9.674 1.00 10.12 7 NATOM 2287 CA ILE D 66 33.361 45.070 9.550 1.00 11.60 6 C ATOM 2288 CBILE D 66 32.970 44.480 10.918 1.00 13.04 6 C ATOM 2289 CG2 ILE D 6631.591 43.825 10.916 1.00 13.13 6 C ATOM 2290 CG1 ILE D 66 34.098 43.48611.300 1.00 13.41 6 C ATOM 2291 CD1 ILE D 66 33.797 42.450 12.339 1.0016.26 6 C ATOM 2292 C ILE D 66 32.247 45.760 8.785 1.00 12.88 6 C ATOM2293 O ILE D 66 31.842 46.893 9.084 1.00 11.94 8 O ATOM 2294 N GLN D 6731.690 45.048 7.820 1.00 13.32 7 N ATOM 2295 CA GLN D 67 30.565 45.5707.031 1.00 13.81 6 C ATOM 2296 CB GLN D 67 30.876 45.313 5.537 1.0017.32 6 C ATOM 2297 CG GLN D 67 32.065 46.094 5.003 1.00 20.78 6 C ATOM2298 CD GLN D 67 32.258 45.972 3.503 1.00 25.96 6 C ATOM 2299 OE1 GLN D67 32.984 46.769 2.874 1.00 26.83 8 O ATOM 2300 NE2 GLN D 67 31.66644.957 2.880 1.00 26.96 7 N ATOM 2301 C GLN D 67 29.249 44.883 7.3661.00 14.11 6 C ATOM 2302 O GLN D 67 28.204 45.520 7.260 1.00 12.99 8 OATOM 2303 N MET D 68 29.234 43.596 7.687 1.00 13.17 7 N ATOM 2304 CA METD 68 27.975 42.918 8.012 1.00 14.61 6 C ATOM 2305 CB MET D 68 27.29242.526 6.707 1.00 17.39 6 C ATOM 2306 CG MET D 68 27.455 41.040 6.4411.00 26.39 6 C ATOM 2307 SD MET D 68 25.983 40.112 6.946 1.00 28.95 16 SATOM 2308 CE MET D 68 25.089 40.321 5.379 1.00 31.95 6 C ATOM 2309 C METD 68 28.269 41.745 8.924 1.00 12.16 6 C ATOM 2310 O MET D 68 29.37441.163 8.887 1.00 8.95 8 O ATOM 2311 N THR D 69 27.352 41.409 9.826 1.0010.00 7 N ATOM 2312 CA THR D 69 27.548 40.286 10.750 1.00 9.18 6 C ATOM2313 CB THR D 69 27.973 40.679 12.180 1.00 10.80 6 C ATOM 2314 OG1 THR D69 26.965 41.517 12.738 1.00 10.14 8 O ATOM 2315 CG2 THR D 69 29.26641.533 12.207 1.00 8.79 6 C ATOM 2316 C THR D 69 26.209 39.551 10.8851.00 10.48 6 C ATOM 2317 O THR D 69 25.177 40.204 10.825 1.00 9.21 8 OATOM 2318 N SER D 70 26.237 38.260 11.134 1.00 8.78 7 N ATOM 2319 CA SERD 70 25.036 37.493 11.456 1.00 11.09 6 C ATOM 2320 CB SER D 70 24.66236.702 10.189 1.00 12.33 6 C ATOM 2321 OG SER D 70 23.493 35.994 10.5101.00 13.28 8 O ATOM 2322 C SER D 70 25.401 36.622 12.648 1.00 12.05 6 CATOM 2323 O SER D 70 26.397 35.870 12.567 1.00 12.32 8 O ATOM 2324 N THRD 71 24.700 36.673 13.769 1.00 10.51 7 N ATOM 2325 CA THR D 71 25.09735.890 14.952 1.00 9.85 6 C ATOM 2326 CB THR D 71 25.203 36.844 16.1561.00 11.18 6 C ATOM 2327 OG1 THR D 71 26.203 37.821 15.911 1.00 8.76 8 OATOM 2328 CG2 THR D 71 25.517 36.186 17.508 1.00 12.67 6 C ATOM 2329 CTHR D 71 24.120 34.795 15.261 1.00 8.82 6 C ATOM 2330 O THR D 71 22.91635.051 15.240 1.00 9.45 8 O ATOM 2331 N MET D 72 24.629 33.588 15.4741.00 6.60 7 N ATOM 2332 CA MET D 72 23.854 32.463 15.846 1.00 7.67 6 CATOM 2333 CB MET D 72 24.175 31.262 14.930 1.00 9.49 6 C ATOM 2334 CGMET D 72 23.687 31.559 13.500 1.00 14.91 6 C ATOM 2335 SD MET D 7224.478 30.502 12.269 1.00 15.37 16 S ATOM 2336 CE MET D 72 26.144 31.12312.202 1.00 17.36 6 C ATOM 2337 C MET D 72 24.124 32.078 17.306 1.007.83 6 C ATOM 2338 O MET D 72 25.184 31.458 17.566 1.00 8.05 8 O ATOM2339 N ILE D 73 23.212 32.379 18.186 1.00 8.09 7 N ATOM 2340 CA ILE D 7323.332 32.016 19.610 1.00 8.99 6 C ATOM 2341 CB ILE D 73 22.410 32.88020.471 1.00 9.51 6 C ATOM 2342 CG2 ILE D 73 22.288 32.354 21.920 1.0011.36 6 C ATOM 2343 CG1 ILE D 73 22.973 34.315 20.535 1.00 11.16 6 CATOM 2344 CD1 ILE D 73 21.936 35.363 20.896 1.00 11.78 6 C ATOM 2345 CILE D 73 23.038 30.542 19.775 1.00 11.33 6 C ATOM 2346 O ILE D 73 22.03630.000 19.267 1.00 11.92 8 O ATOM 2347 N ALA D 74 23.949 29.824 20.4141.00 12.81 7 N ATOM 2348 CA ALA D 74 23.809 28.415 20.674 1.00 16.28 6 CATOM 2349 CB ALA D 74 25.122 27.728 21.084 1.00 17.94 6 C ATOM 2350 CALA D 74 22.875 28.195 21.886 1.00 18.40 6 C ATOM 2351 O ALA D 74 22.93628.914 22.879 1.00 16.19 8 O ATOM 2352 N ILE D 75 22.208 27.070 21.8261.00 20.41 7 N ATOM 2353 CA ILE D 75 21.496 26.531 22.968 1.00 24.59 6 CATOM 2354 CB ILE D 75 20.391 25.581 22.489 1.00 25.97 6 C ATOM 2355 CG2ILE D 75 20.115 24.427 23.439 1.00 26.43 6 C ATOM 2356 CG1 ILE D 7519.126 26.385 22.194 1.00 24.83 6 C ATOM 2357 CD1 ILE D 75 19.021 26.71720.709 1.00 27.91 6 C ATOM 2358 C ILE D 75 22.520 25.792 23.826 1.0027.53 6 C ATOM 2359 O ILE D 75 23.522 25.173 23.340 1.00 29.12 8 O ATOM2360 OT ILE D 75 22.263 25.858 25.044 1.00 29.73 8 O ATOM 2361 O HOH W 110.122 48.309 2.632 1.00 71.67 8 O ATOM 2362 O HOH W 2 43.515 40.3354.275 1.00 2.02 8 O ATOM 2363 O HOH W 3 25.096 40.046 14.391 1.00 4.72 8O ATOM 2364 O HOH W 4 14.309 48.257 1.287 1.00 11.98 8 O ATOM 2365 O HOHW 5 39.903 40.707 3.072 1.00 4.59 8 O ATOM 2366 O HOH W 6 13.993 28.92716.050 1.00 8.16 8 O ATOM 2367 O HOH W 7 −2.824 44.331 8.615 1.00 6.77 8O ATOM 2368 O HOH W 8 46.758 44.137 12.543 1.00 9.80 8 O ATOM 2369 O HOHW 9 22.057 38.363 30.808 1.00 10.53 8 O ATOM 2370 O HOH W 10 41.58830.809 8.960 1.00 14.97 8 O ATOM 2371 O HOH W 11 32.061 36.737 −0.4381.00 13.10 8 O ATOM 2372 O HOH W 12 25.621 43.699 12.042 1.00 12.21 8 OATOM 2373 O HOH W 13 41.643 43.045 19.819 1.00 12.14 8 O ATOM 2374 O HOHW 14 18.598 35.090 17.431 1.00 7.05 8 O ATOM 2375 O HOH W 15 35.64246.870 0.589 1.00 14.25 8 O ATOM 2376 O HOH W 16 −2.309 45.120 15.3491.00 5.19 8 O ATOM 2377 O HOH W 17 21.148 34.153 17.144 1.00 9.28 8 OATOM 2378 O HOH W 18 10.042 49.433 21.052 1.00 16.66 8 O ATOM 2379 O HOHW 19 24.907 43.442 9.509 1.00 8.06 8 O ATOM 2380 O HOH W 20 40.34029.254 22.526 1.00 15.14 8 O ATOM 2381 O HOH W 21 24.110 53.078 16.0161.00 13.14 8 O ATOM 2382 O HOH W 22 30.704 31.905 3.919 1.00 15.56 8 OATOM 2383 O HOH W 23 16.095 40.414 25.005 1.00 19.01 8 O ATOM 2384 O HOHW 24 −4.341 51.429 0.089 1.00 10.32 8 O ATOM 2385 O HOH W 25 34.57738.997 0.926 1.00 10.61 8 O ATOM 2386 O HOH W 26 31.432 48.786 11.6761.00 11.01 8 O ATOM 2387 O HOH W 27 32.097 37.715 29.475 1.00 10.30 8 OATOM 2388 O HOH W 28 −2.898 39.485 16.073 1.00 12.22 8 O ATOM 2389 O HOHW 29 22.459 34.856 30.472 1.00 17.04 8 O ATOM 2390 O HOH W 30 −10.08435.455 15.693 1.00 11.27 8 O ATOM 2391 O HOH W 31 44.404 44.372 13.6871.00 7.67 8 O ATOM 2392 O HOH W 32 30.987 42.125 27.230 1.00 18.75 8 OATOM 2393 O HOH W 33 24.805 29.509 24.797 1.00 10.67 8 O ATOM 2394 O HOHW 34 13.345 25.150 11.364 1.00 12.88 8 O ATOM 2395 O HOH W 35 44.40442.640 6.498 1.00 6.66 8 O ATOM 2396 O HOH W 36 13.422 56.153 −1.7491.00 15.97 8 O ATOM 2397 O HOH W 37 19.476 35.360 30.724 1.00 11.73 8 OATOM 2398 O HOH W 38 9.109 42.563 1.635 1.00 6.86 8 O ATOM 2399 O HOH W39 40.432 23.485 5.437 1.00 12.80 8 O ATOM 2400 O HOH W 40 42.096 30.24011.776 1.00 14.42 8 O ATOM 2401 O HOH W 41 42.873 37.972 25.858 1.0011.47 8 O ATOM 2402 O HOH W 42 9.770 32.553 31.637 1.00 15.13 8 O ATOM2403 O HOH W 43 −6.902 49.549 6.190 1.00 9.47 8 O ATOM 2404 O HOH W 4428.555 30.858 2.221 1.00 13.41 8 O ATOM 2405 O HOH W 45 −1.387 38.89922.433 1.00 11.37 8 O ATOM 2406 O HOH W 46 37.404 40.600 2.609 1.0014.02 8 O ATOM 2407 O HOH W 47 22.531 31.796 29.779 1.00 17.69 8 O ATOM2408 O HOH W 48 23.048 38.797 14.218 1.00 14.69 8 O ATOM 2409 O HOH W 4917.943 35.402 10.729 1.00 20.01 8 O ATOM 2410 O HOH W 50 5.174 54.657−2.822 1.00 17.90 8 O ATOM 2411 O HOH W 51 13.246 20.750 19.283 1.0029.29 8 O ATOM 2412 O HOH W 52 48.032 37.211 11.489 1.00 16.87 8 O ATOM2413 O HOH W 53 30.209 20.178 20.611 1.00 18.81 8 O ATOM 2414 O HOH W 5423.513 22.824 21.491 1.00 20.26 8 O ATOM 2415 O HOH W 55 21.561 29.53025.223 1.00 20.52 8 O ATOM 2416 O HOH W 56 42.111 36.443 28.208 1.0019.47 8 O ATOM 2417 O HOH W 57 40.876 29.661 15.978 1.00 16.06 8 O ATOM2418 O HOH W 58 16.494 34.453 13.028 1.00 25.55 8 O ATOM 2419 O HOH W 59−0.857 46.408 17.858 1.00 16.37 8 O ATOM 2420 O HOH W 60 32.821 25.9710.795 1.00 16.71 8 O ATOM 2421 O HOH W 61 45.627 45.117 6.519 1.00 18.948 O ATOM 2422 O HOH W 62 21.976 40.997 13.661 1.00 17.37 8 O ATOM 2423 OHOH W 63 15.836 30.099 15.388 1.00 15.61 8 O ATOM 2424 O HOH W 64 30.46926.207 24.253 1.00 22.51 8 O ATOM 2425 O HOH W 65 16.718 46.859 20.9291.00 14.88 8 O ATOM 2426 O HOH W 66 10.425 43.686 25.115 1.00 19.64 8 OATOM 2427 O HOH W 67 21.112 30.369 11.432 1.00 24.23 8 O ATOM 2428 O HOHW 68 17.191 27.886 14.604 1.00 16.40 8 O ATOM 2429 O HOH W 69 −1.31355.974 8.469 1.00 20.48 8 O ATOM 2430 O HOH W 70 −5.616 49.292 2.5461.00 14.23 8 O ATOM 2431 O HOH W 71 18.470 59.447 0.811 1.00 16.23 8 OATOM 2432 O HOH W 72 4.679 43.284 24.130 1.00 26.97 8 O ATOM 2433 O HOHW 73 28.210 48.003 7.105 1.00 25.13 8 O ATOM 2434 O HOH W 74 24.47250.097 17.032 1.00 18.43 8 O ATOM 2435 O HOH W 75 44.769 47.165 4.7121.00 20.42 8 O ATOM 2436 O HOH W 76 20.456 33.034 10.801 1.00 21.21 8 OATOM 2437 O HOH W 77 27.096 24.676 0.583 1.00 16.78 8 O ATOM 2438 O HOHW 78 45.463 43.772 2.754 1.00 19.58 8 O ATOM 2439 O HOH W 79 36.90242.152 24.314 1.00 19.89 8 O ATOM 2440 O HOH W 80 25.028 31.904 31.0351.00 25.70 8 O ATOM 2441 O HOH W 81 19.951 31.779 31.151 1.00 20.25 8 OATOM 2442 O HOH W 82 2.821 56.599 5.110 1.00 24.51 8 O ATOM 2443 O HOH W83 0.240 38.318 26.532 1.00 20.61 8 O ATOM 2444 O HOH W 84 −5.501 28.9628.643 1.00 25.67 8 O ATOM 2445 O HOH W 85 15.080 16.024 18.827 1.0023.79 8 O ATOM 2446 O HOH W 86 17.453 52.249 2.615 1.00 24.26 8 O ATOM2447 O HOH W 87 10.408 41.106 29.551 1.00 25.17 8 O ATOM 2448 O HOH W 88−1.008 36.087 24.249 1.00 22.03 8 O ATOM 2449 O HOH W 89 28.715 28.4201.496 1.00 33.15 8 O ATOM 2450 O HOH W 90 26.479 48.310 17.176 1.0014.80 8 O ATOM 2451 O HOH W 91 4.544 21.028 22.060 1.00 23.39 8 O ATOM2452 O HOH W 92 7.062 20.090 15.336 1.00 17.95 8 O ATOM 2453 O HOH W 93−8.087 52.015 8.198 1.00 13.31 8 O ATOM 2454 O HOH W 94 13.718 42.09626.908 1.00 15.23 8 O ATOM 2455 O HOH W 95 −5.925 31.260 22.875 1.0018.50 8 O ATOM 2456 O HOH W 96 2.262 47.977 19.728 1.00 22.03 8 O ATOM2457 O HOH W 97 39.222 48.045 1.791 1.00 23.00 8 O ATOM 2458 O HOH W 9813.202 60.207 12.694 1.00 18.82 8 O ATOM 2459 O HOH W 99 35.592 47.97518.124 1.00 21.60 8 O ATOM 2460 O HOH W 100 32.405 49.718 9.091 1.0023.23 8 O ATOM 2461 O HOH W 101 32.530 40.013 27.354 1.00 26.88 8 O ATOM2462 O HOH W 102 15.214 19.032 19.403 1.00 31.35 8 O ATOM 2463 O HOH W103 29.693 49.421 9.197 1.00 18.95 8 O ATOM 2464 O HOH W 104 34.77034.970 27.712 1.00 36.84 8 O ATOM 2465 O HOH W 105 13.525 57.916 19.0611.00 24.00 8 O ATOM 2466 O HOH W 106 22.201 34.397 12.453 1.00 27.96 8 OATOM 2467 O HOH W 107 35.580 49.792 6.450 1.00 17.81 8 O ATOM 2468 O HOHW 108 28.722 38.669 0.882 1.00 28.33 8 O ATOM 2469 O HOH W 109 −1.55226.414 23.825 1.00 25.54 8 O ATOM 2470 O HOH W 110 6.586 43.932 26.8461.00 30.40 8 O ATOM 2471 O HOH W 111 26.222 21.733 1.726 1.00 27.89 8 OATOM 2472 O HOH W 112 38.816 11.853 6.953 1.00 31.38 8 O ATOM 2473 O HOHW 113 40.804 10.936 −0.338 1.00 43.84 8 O ATOM 2474 O HOH W 114 14.46523.160 5.012 1.00 35.94 8 O ATOM 2475 O HOH W 115 29.274 51.396 12.6341.00 31.23 8 O ATOM 2476 O HOH W 116 36.111 26.053 21.704 1.00 27.88 8 OATOM 2477 O HOH W 117 48.720 40.919 8.861 1.00 27.14 8 O ATOM 2478 O HOHW 118 16.857 35.240 8.265 1.00 29.17 8 O ATOM 2479 O HOH W 119 40.27215.140 −2.430 1.00 28.86 8 O ATOM 2480 O HOH W 120 8.464 39.395 3.4691.00 26.29 8 O ATOM 2481 O HOH W 121 24.991 50.953 19.868 1.00 38.39 8 OATOM 2482 O HOH W 122 32.494 43.852 25.475 1.00 20.94 8 O ATOM 2483 OHOH W 123 42.907 20.569 5.948 1.00 20.98 8 O ATOM 2484 O HOH W 12411.409 60.427 −4.212 1.00 22.37 8 O ATOM 2485 O HOH W 125 41.932 17.910−0.484 1.00 24.91 8 O ATOM 2486 O HOH W 126 −6.114 27.302 10.662 1.0025.66 8 O ATOM 2487 O HOH W 127 26.137 21.371 21.978 1.00 29.82 8 O ATOM2488 O HOH W 128 8.216 61.214 10.723 1.00 30.53 8 O ATOM 2489 O HOH W129 26.118 11.522 20.391 1.00 26.63 8 O ATOM 2490 O HOH W 130 38.98615.328 −4.568 1.00 35.28 8 O ATOM 2491 O HOH W 131 32.545 51.686 12.9971.00 34.45 8 O ATOM 2492 O HOH W 132 10.691 28.090 29.628 1.00 23.73 8 OATOM 2493 O HOH W 133 42.534 51.095 7.222 1.00 27.35 8 O ATOM 2494 O HOHW 134 24.319 44.661 27.426 1.00 29.68 8 O ATOM 2495 O HOH W 135 27.18427.908 26.119 1.00 29.01 8 O ATOM 2496 O HOH W 136 12.482 23.286 9.7181.00 25.90 8 O ATOM 2497 O HOH W 137 13.725 58.650 6.382 1.00 30.09 8 OATOM 2498 O HOH W 138 16.824 30.085 33.861 1.00 29.63 8 O ATOM 2499 OHOH W 139 25.538 40.408 32.871 1.00 25.50 8 O ATOM 2500 O HOH W 140−4.601 42.598 6.460 1.00 28.50 8 O ATOM 2501 O HOH W 141 −4.314 55.8001.473 1.00 23.98 8 O ATOM 2502 O HOH W 142 4.180 40.729 2.968 1.00 38.798 O ATOM 2503 O HOH W 143 2.880 34.275 3.975 1.00 35.32 8 O ATOM 2504 OHOH W 144 22.685 41.116 9.871 1.00 37.58 8 O ATOM 2505 O HOH W 145 5.91142.222 1.293 1.00 38.18 8 O ATOM 2506 O HOH W 146 −0.037 32.781 6.9461.00 36.95 8 O ATOM 2507 O HOH W 147 9.637 20.349 10.139 1.00 25.04 8 OATOM 2508 O HOH W 148 28.382 43.815 3.179 1.00 43.71 8 O ATOM 2509 O HOHW 149 22.090 59.094 6.799 1.00 36.67 8 O ATOM 2510 O HOH W 150 10.61240.037 0.986 1.00 30.45 8 O ATOM 2511 O HOH W 151 33.822 49.094 4.6721.00 31.89 8 O ATOM 2512 O HOH W 152 10.343 15.118 17.380 1.00 41.68 8 OATOM 2513 O HOH W 153 17.037 38.046 31.684 1.00 36.09 8 O ATOM 2514 OHOH W 154 −4.211 57.650 5.730 1.00 37.59 8 O ATOM 2515 O HOH W 15510.360 30.679 5.056 1.00 35.82 8 O ATOM 2516 O HOH W 156 9.319 30.37730.150 1.00 45.13 8 O ATOM 2517 O HOH W 157 32.537 46.738 25.670 1.0042.21 8 O ATOM 2518 O HOH W 158 −7.040 33.392 21.564 1.00 29.97 8 O ATOM2519 O HOH W 159 25.487 57.687 13.423 1.00 56.41 8 O ATOM 2520 O HOH W160 22.390 27.493 27.703 1.00 51.26 8 O ATOM 2521 O HOH W 161 −1.94432.594 29.002 1.00 35.60 8 O ATOM 2522 O HOH W 162 19.494 57.986 15.7031.00 27.66 8 O ATOM 2523 O HOH W 163 4.218 34.783 −0.031 1.00 37.95 8 OATOM 2524 O HOH W 164 10.878 61.676 2.439 1.00 40.13 8 O ATOM 2525 O HOHW 165 32.378 32.684 29.566 1.00 39.10 8 O ATOM 2526 O HOH W 166 40.74227.829 18.701 1.00 32.58 8 O ATOM 2527 O HOH W 167 15.407 53.314 22.6741.00 35.33 8 O ATOM 2528 O HOH W 168 24.525 50.894 4.209 1.00 30.85 8 OATOM 2529 O HOH W 169 24.227 47.558 13.850 1.00 13.12 8 O ATOM 2530 OHOH W 170 19.469 40.382 25.052 1.00 14.83 8 O ATOM 2531 O HOH W 17125.449 45.435 13.288 1.00 34.42 8 O ATOM 2532 O HOH W 172 36.869 45.89618.115 1.00 21.20 8 O ATOM 2533 O HOH W 173 12.012 28.477 9.138 1.0029.45 8 O ATOM 2534 O HOH W 174 16.359 41.479 3.585 1.00 38.60 8 O ATOM2535 O HOH W 175 34.326 31.763 −2.161 1.00 2.02 8 O ATOM 2536 O HOH W176 17.655 52.366 −0.174 1.00 22.73 8 O ATOM 2537 O HOH W 177 14.40448.517 21.739 1.00 25.45 8 O ATOM 2538 O HOH W 178 43.749 32.200 8.0201.00 29.02 8 O ATOM 2539 O HOH W 179 15.592 26.829 16.174 1.00 48.16 8 OATOM 2540 O HOH W 180 10.525 56.368 −4.081 1.00 30.23 8 O ATOM 2541 OHOH W 181 8.713 25.942 28.348 1.00 25.77 8 O ATOM 2542 O HOH W 18229.737 7.237 11.850 1.00 33.68 8 O ATOM 2543 O HOH W 183 21.164 33.4397.966 1.00 38.28 8 O ATOM 2544 O HOH W 184 24.119 46.149 24.377 1.0037.23 8 O ATOM 2545 O HOH W 185 21.753 56.692 10.121 1.00 33.11 8 O ATOM2546 O HOH W 186 17.585 38.448 12.402 1.00 39.35 8 O ATOM 2547 O HOH W187 −2.754 38.361 11.916 1.00 44.68 8 O ATOM 2548 O HOH W 188 16.63927.328 18.235 1.00 24.46 8 O ATOM 2549 O HOH W 189 14.615 34.256 8.5341.00 41.84 8 O ATOM 2550 O HOH W 190 14.514 43.801 25.157 1.00 33.67 8 OATOM 2551 O HOH W 191 26.469 30.002 3.866 1.00 53.62 8 O ATOM 2552 O HOHW 192 3.416 28.107 4.131 1.00 47.37 8 O ATOM 2553 O HOH W 193 15.41427.531 11.247 1.00 40.29 8 O ATOM 2554 O HOH W 194 24.628 58.221 6.0321.00 37.02 8 O ATOM 2555 O HOH W 195 −1.971 51.690 17.147 1.00 35.28 8 OATOM 2556 O HOH W 196 16.659 23.680 26.303 1.00 37.61 8 O END

All publications, patents, and patent applications cited herein areincorporated herein by reference in their entirety.

Industrial Applicability

Lrp-like proteins activate or inactivate transcription of a wide rangeof genes of microorganisms, which are involved in metabolism or growthof microorgansims, or their infection. Accordingly, a ligand that altersthe 3D structure of an Lrp-like protein, or that regulates the functionof an Lrp-like protein by changing its 3D structure or by affecting itsability to assemble, can be used as a pharmaceutical agent in order toregulate metabolism or, growth of, or infection by microorganisms.

So far no Lrp-like protein has been found in eukaryotes, includinghumans. Accordingly, by the use of such a ligand no serious side effectsis expected to humans. The target of the ligand, i.e., an Lrp-likeprotein, and its mechanism of action are different from those of knownantibiotics. This might prevent infection by microorganisms which areresistant to known antibiotics; such infection is becoming more and moreserious.

The present invention provides a highly useful method for screening forthe aforementioned ligand. Thus, this can serve as an innovative methodfor developing pharmaceutical agents that can complement knownantibiotics through a radically new approach.

Free Text of Sequence Listing

SEQ ID NO: 1: description of artificial sequence: primer

SEQ ID NO: 2: description of artificial sequence: primer

1. A method of screening for pharmaceutical agents that regulate atleast one of metabolism or growth of, or infection by microorganisms,which comprises selecting a ligand of an Lrp-like protein based on 3Dstructural information of the protein, wherein the 3D structuralinformation is information obtained from a crystallized complexcomprising an assembly of the Lrp-like protein and at least one naturalligand.
 2. The method according to claim 1, wherein the 3D structuralinformation is information concerning gaps or pockets generated from anassembly of the Lrp-like protein.
 3. The method according to claim 1,wherein the 3D structural information is information obtained from 3Datomic coordinates determined by X-ray diffraction analysis.
 4. Themethod according to claim 1, which comprises the following steps: a)determination of the 3D atomic coordinates of the Lrp-like protein byapplying X-ray diffraction analysis to the protein crystallized; b)processing of the atomic coordinates using a program designed foranalyzing protein 3D structures, thereby obtaining graphical and/ornumerical information concerning the 3D structure of the Lrp-likeprotein; c) deduction of the 3D structural information on gaps orpockets generated by assembling of the Lrp-like protein, by using thegraphical and/or numerical information; and d) identification of ligandswhich will interact with the Lrp-like protein and thus will be usable aspharmaceutical agents, by using the 3D structural information.
 5. Themethod according to claim 1, wherein the pharmaceutical agents thatregulate at least one of metabolism or growth of, or infection bymicroorganisms, are antimicrobial agents.
 6. The method according toclaim 1, wherein the Lrp-like protein is derived from a microorganismbelonging to the genus Pyrococcus.
 7. The method according to claim 6,wherein the microorganism is Pyrococcus sp. OT3 (JCM 9974).
 8. Themethod according to claim 7, wherein the 3D atomic coordinates comprisethe data shown in Table
 1. 9-12. (canceled)
 13. The method according toclaim 1, wherein the assembly of the Lrp-like protein is an octamer ofthe Lrp-like protein.
 14. The method according to claim 13, wherein theligand is able to stabilize the octamer of the Lrp-like protein.
 15. Acrystallized complex comprising an assembly of an Lrp-like protein andat least one natural ligand.
 16. The crystallized complex according toclaim 15, wherein the assembly of the Lrp-like protein is an octamer ofthe Lrp-like protein.
 17. The crystallized complex according to claim15, wherein the Lrp-like protein is derived from a microorganismbelonging to the genus Pyrococcus.
 18. The crystallized complexaccording to claim 17, wherein the microorganism is Pyrococcus sp. OT3(JCM 9974).
 19. The crystallized complex according to claim 15, whereinthe crystallized complex is defined by the data shown in Table 1.